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Difference between revisions of "O2k-Peltier Temperature Control"

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{{Product
{{Technical support}}
|description='''O2k-Peltier Temperature Control''': Built-in electronic thermostat controlling temperature for two [[O2k-Chamber]]s in the range of 4 to 47 °C; ±0.002 °C (at room temperature).  Continuous recording of the O2k-Copper Block temperature with DatLab. Temperature change from 20 to 30 °C within 15 min; cooling from 30 to 20 °C within 20 min. Integral component of the [[O2k-Main Unit]]. The electronic temperature control of the O2k replaced the conventional water jacket.
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|product id=20020
{{MitoPedia without banner
|product type=O2k-Main Unit
|abbr=
|info=[[O2k-Core]], [[O2k-Peltier Mitopedia]]
|description='''O2k-Peltier Temperature Control''': Built-in electronic thermostat controlling temperature for two [[O2k-chamber]]s in the range of 4 to 47 °C; ±0.002 °C (at room temperature).  Continuous recording of the O2k-Copper Block temperature with DatLab. Temperature change from 20 to 30 °C within 15 min; cooling from 30 to 20 °C within 20 min. Integral component of the [[O2k-Main Unit]]. The electronic temperature control of the O2k replaced the conventional water jacket.
|info=[[O2k-Core]]
}}
}}
== Lower limit of experimental temperature ==
::::* From O2k-Series E there is a more efficient temperature management system in comparison compared to previous series. At an ambient temperature of 21.1 °C, a stable Peltier power of about 83% reached 0 °C from 37 °C in about 1 h.
::::* For an ambient temperature of 25 °C,  the O2k-Chamber can reach lower temperatures down to 4 °C. The use of temperatures lower than 4 °C is not within our O2k-specifications, as such the quality control parameters under these conditions are not guaranteed. We recommend taking special precautions with a set temperature of 0.5 °C as it could potentially damage the O2k-Chambers or any other component.
::::* For experiments at 0 °C, the O2k can be exposed to ambient temperatures slightly above 0 °C (all the electronic components are evaluated under minimum temperatures of 0 °C, which should be avoided due to condensation concerns). For low room temperatures close to 0 °C a humidity control system should be used.
:::: With O2k-Series D a stable chamber temperature of 0 °C was obtained at a room temperature of ~14 °C.  The cooling power of the Peltier element reached 95% of its maximum capacity.
::::* To use temperatures lower than our O2k-specifications , see [[MiPNet06.05 Test Experiments on O2k-Specifications| test experiments on O2k-Specifications]]. Please note that to reach a lower ambient temperature needed for experiments at 2 °C, a cold room is not necessarily required; an air-conditioned room should suffice.
== Troubleshooting ==
=== Block temperature does not increase while heating with full power ===
::::* '''Customer ID''': Stier A, [[FI_Turku_Nikinmaa M]]
::::'''Question:'''
Our O2k (likely bought in 2004) seems to have a problem of temperature control (error message + T°C staying at RT despite a set T°C of 40°C). Following the instructions of [[DatLab_error_messages]]; I switched it off and on (likely > 10 times), tried with an older version of the software and different temperature (20 to 40°C), but nothing seems to fix the issue. Representative data is attached (2020-04-22).
[[File:Ticket-2020042210000011 O2k Peltier.png|center|700px]]
::::'''Answer:''' See [[DatLab_error_messages#DatLab_error:_Average_block_temperature_over_past_10_min_has_not_reached_set_temperature|DatLab error: Average block temperature over past 10 min has not reached set temperature]] and [[DatLab_error_messages#DatLab_error:_Block_temperature_does_not_increase_while_heating_with_full_power|DatLab error: Block temperature does not increase while heating with full power]]. If the problem persists, the O2k-Peltier Temperature Control has an electronic defect and the O2k-Main Unit has to be sent to the electronics workshop of OROBOROS for service.
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{{MitoPedia O2k and high-resolution respirometry
{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware
|mitopedia O2k and high-resolution respirometry=O2k hardware
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{{Technical support integrated}}

Latest revision as of 15:28, 10 May 2021


                  


O2k-Open Support

O2k-Peltier Temperature Control



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

O2k-Peltier Temperature Control: Built-in electronic thermostat controlling temperature for two O2k-chambers in the range of 4 to 47 °C; ±0.002 °C (at room temperature). Continuous recording of the O2k-Copper Block temperature with DatLab. Temperature change from 20 to 30 °C within 15 min; cooling from 30 to 20 °C within 20 min. Integral component of the O2k-Main Unit. The electronic temperature control of the O2k replaced the conventional water jacket.


Reference: O2k-Core


Lower limit of experimental temperature

  • From O2k-Series E there is a more efficient temperature management system in comparison compared to previous series. At an ambient temperature of 21.1 °C, a stable Peltier power of about 83% reached 0 °C from 37 °C in about 1 h.
  • For an ambient temperature of 25 °C, the O2k-Chamber can reach lower temperatures down to 4 °C. The use of temperatures lower than 4 °C is not within our O2k-specifications, as such the quality control parameters under these conditions are not guaranteed. We recommend taking special precautions with a set temperature of 0.5 °C as it could potentially damage the O2k-Chambers or any other component.
  • For experiments at 0 °C, the O2k can be exposed to ambient temperatures slightly above 0 °C (all the electronic components are evaluated under minimum temperatures of 0 °C, which should be avoided due to condensation concerns). For low room temperatures close to 0 °C a humidity control system should be used.
With O2k-Series D a stable chamber temperature of 0 °C was obtained at a room temperature of ~14 °C. The cooling power of the Peltier element reached 95% of its maximum capacity.
  • To use temperatures lower than our O2k-specifications , see test experiments on O2k-Specifications. Please note that to reach a lower ambient temperature needed for experiments at 2 °C, a cold room is not necessarily required; an air-conditioned room should suffice.


Troubleshooting

Block temperature does not increase while heating with full power

Question:

Our O2k (likely bought in 2004) seems to have a problem of temperature control (error message + T°C staying at RT despite a set T°C of 40°C). Following the instructions of DatLab_error_messages; I switched it off and on (likely > 10 times), tried with an older version of the software and different temperature (20 to 40°C), but nothing seems to fix the issue. Representative data is attached (2020-04-22).

Ticket-2020042210000011 O2k Peltier.png
Answer: See DatLab error: Average block temperature over past 10 min has not reached set temperature and DatLab error: Block temperature does not increase while heating with full power. If the problem persists, the O2k-Peltier Temperature Control has an electronic defect and the O2k-Main Unit has to be sent to the electronics workshop of OROBOROS for service.








MitoPedia O2k and high-resolution respirometry: O2k hardware