Difference between revisions of "O2k-Videosupport"

Revision as of 10:17, 17 October 2020

O2k-Videosupport

» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.

» This cannot replace a careful study of the O2k-Manual.

O2k-start (DatLab 7/Series H)

MiPNet22.11 O2k-FluoRespirometer manual

O2k ready to use

A1 Oroboros flash drive and DatLab 7 installation

A2 Insert O2k Chamber

A3 Insert OroboPOS

A4 O2k-chamber volume calibration

A5 DL7 Start DatLab7

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start

Insert O2k-Chamber

Insert OroboPOS

O2k-Chamber volume calibration

O2k ready to use

POS-service and Chamber-service (all series)

POS-service

MiPNet19.18B POS-service

OroboPOS from Perspex housing

Disassembly of OroboPOS

Chamber-service

Chamber service Insert O2k Chamber

Chamber service O2k Chamber volume calibration

DatLab 7.4

How to find a DL-Protocol (DLP)

How to perform an experiment with a SUIT DL-Protocol (DLP)

O₂ flux analysis with DatLab 7.4

Export DL-Protocol User (*.DLPU)

O2k-Mitochondrial preparations

MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers

Shredder tissue homogenate

Fiber permeabilization

Q-Module (NextGen-O2k - DatLab 7)

Q-service

Assembly of the reference electrode

NG Polishing glassy carbon and platinum electrodes

NG O2k-Chamber volume calibration with Q-Sensor

Q-experiments

NG Q-experiments

Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry

NG Cleaning after Q-experiments

Exclusive material for O2k-Workshop

O2k Quality Control

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

O2k-Mitochondrial preparations

Addition of biological sample to the respirometer chamber

FluoRespirometry

Smart Fluo-Sensor assembly

Exchanging a filter set of the smart-fluo sensor

H₂O₂ flux analysis with DatLab 7.4

@@ Line 1: / Line 1: @@
-{{OROBOROS_header_page_name}}
+{{Template:OROBOROS support page name}}
+{{NextGen-O2k H2020-support}}
+[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
+::» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
+::::» This cannot replace a careful study of the O2k-Manual.
+<br>
-The OROBOROS Videosupport is an addition to the '''[[O2k-Manual| O2k-Manuals]]''' and cannot replace their carful study.
+__TOC__
+<!--
+https://www.mediawiki.org/wiki/Extension:Replace_Text
+-->
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== O2k-start (DatLab 7/Series H) ==
+<div class="mw-collapsible-content">
+[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 '''A4''' O2k-chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
-'''O2k-Start'''
-* [https://www.youtube.com/watch?v=VBAtznkQ5cs A1 OROBOROS flash drive and DatLab installation]
+</div>
-* [https://www.youtube.com/watch?v=KlxdzoDV1EY A2 Start DatLab]
+</div>
-* [https://www.youtube.com/watch?v=1JffCpapX6A A3 Insert O2k-Chamber]
-* [https://www.youtube.com/watch?v=cjsdZbvT8qc A4 Insert OroboPOS]
+<br/>
-* [https://www.youtube.com/watch?v=UsNZ8qsiFbA A5 O2k-Chamber volume calibration]
+[[File:Expand.png|right|45px |Click to expand or collaps]]
-* [https://www.youtube.com/watch?v=fa0coSjXBBw A6 O2k ready to use]
+<div class="toccolours mw-collapsible mw-collapsed">
+== O2k-start (DatLab 6/Series G) ==
+<div class="mw-collapsible-content">
+[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
-'''POS-Service'''
+</div>
-* [https://www.youtube.com/watch?v=Yso5KZUvefk B1 OroboPOS from Perspex housing]
+</div>
-* [https://www.youtube.com/watch?v=WWm9bAr2hNQ B2 Disassembly of OroboPOS]
-* [https://www.youtube.com/watch?v=GmAfRzpbHs8 B3 Cathode cleaning]
+<br/>
-* [https://www.youtube.com/watch?v=855Q--AZmYo B4 Anode cleaning]
+[[File:Expand.png|right|45px |Click to expand or collaps]]
-* [https://www.youtube.com/watch?v=KdSVajj0tSs B5 Membrane mounting]
+<div class="toccolours mw-collapsible">
+== POS-service and Chamber-service (all series) ==
+<div class="mw-collapsible-content">
+* '''POS-service'''
+[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
+---------
+* ''' Chamber-service'''
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== DatLab 7.4 ==
+<div class="mw-collapsible-content">
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be  How to perform an experiment with a SUIT DL-Protocol (DLP)]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== O2k-Mitochondrial preparations ==
+<div class="mw-collapsible-content">
+[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
+[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
+[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== Q-Module (NextGen-O2k - DatLab 7) ==
+<div class="mw-collapsible-content">
+* '''Q-service'''
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
+---------
+* ''' Q-experiments'''
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
+---------
+* ''' Cleaning Q-Sensors'''
+[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
+[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== Exclusive material for O2k-Workshop ==
+<div class="mw-collapsible-content">
+* '''O2k Quality Control'''
+[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
+---------
+* '''O2k-Mitochondrial preparations'''
+[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber
+---------
+* '''FluoRespirometry'''
+[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly
+[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor
+[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4
+</div>
+</div>
+</div>
+</div>
+<br/>