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Difference between revisions of "O2k-Videosupport"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[Image:O2k-Manual.jpg|right|200px|link=http://wiki.oroboros.at/index.php/O2k-Manual |O2k-Manual]]
{{NextGen-O2k H2020-support}}
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
::Β» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
::::Β» This cannot replace a careful study of the O2k-Manual.
<br>
Β 
__TOC__
__TOC__
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== O2k-start (DatLab 7/Series H) ==
<div class="mw-collapsible-content">
[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]


:::: Complementary to the '''[[O2k-Manual]]''', the O2k-Videosupport provides valuable assistance.
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]


:::: This cannot replace a careful study of the O2k-Manual.
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 '''A4''' O2k-chamber volume calibration]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]


</div>
</div>


== [[MiPNet19.18A O2k-Series G: Start]] ==
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
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== O2k-start (DatLab 6/Series G) ==
<div class="mw-collapsible-content">


[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber] Β 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber] Β 
::::* ''New'': O2k-Series H is delivered with the O2k-Chambers mounted. Therefore, mounting is only necessary after removing the glass chamber for a full cleaning service. This is required for regular [[MiPNet19.03 O2k-cleaning and ISS |cleaning the glass chamber]].


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]
::::* ''New'': O2k-Series H is delivered with volume-calibrated O2k-Chambers. Therefore, volume calibration is only necessary for specific stoppers of the [[O2k-TPP+ ISE-Module]], the [[O2k-pH ISE-Module]], and the [[O2k-NO Amp-Module]].


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
Β  Β 
Β  Β 
</div>
</div>
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== POS-service and Chamber-service (all series) ==
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* '''POS-service'''
[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
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* ''' Chamber-service'''
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
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<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
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== DatLab 7.4 ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.beΒ  How to perform an experiment with a SUIT DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
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== O2k-Mitochondrial preparations ==
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[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.beΒ  Fiber permeabilization]
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== Q-Module (NextGen-O2k - DatLab 7) ==
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* '''Q-service'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.beΒ  Assembly of the reference electrode]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
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* ''' Q-experiments'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
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* ''' Cleaning Q-Sensors'''
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
</div>
</div>
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== Exclusive material for O2k-Workshop ==
<div class="mw-collapsible-content">
* '''O2k Quality Control'''
[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
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* '''O2k-Mitochondrial preparations'''
[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber
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* '''FluoRespirometry'''


== [[MiPNet19.18B POS-service]] ==
[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Yso5KZUvefk]] [https://www.youtube.com/watch?v=Yso5KZUvefk B1 OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=WWm9bAr2hNQ]] [https://www.youtube.com/watch?v=WWm9bAr2hNQ B2 Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GmAfRzpbHs8]] [https://www.youtube.com/watch?v=GmAfRzpbHs8 B3 Cathode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=855Q--AZmYo]] [https://www.youtube.com/watch?v=855Q--AZmYo B4 Anode cleaning]
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=KdSVajj0tSs]] [https://www.youtube.com/watch?v=KdSVajj0tSs B5 Membrane mounting]
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Revision as of 09:17, 17 October 2020


                  


O2k-Open Support

O2k-Videosupport


Template NextGen-O2k.jpg
O2k-Videosupport
Β» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
Β» This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps



Click to expand or collaps

Exclusive material for O2k-Workshop


  • O2k Quality Control


PlayVideo.jpg O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration



  • O2k-Mitochondrial preparations


PlayVideo.jpg Addition of biological sample to the respirometer chamber



  • FluoRespirometry


PlayVideo.jpg Smart Fluo-Sensor assembly

PlayVideo.jpg Exchanging a filter set of the smart-fluo sensor

PlayVideo.jpg H2O2 flux analysis with DatLab 7.4