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Difference between revisions of "O2k-Videosupport"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[Image:O2k-Manual.jpg|right|200px|link=http://wiki.oroboros.at/index.php/O2k-Manual |O2k-Manual]]
{{NextGen-O2k H2020-support}}
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
::ยป The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
::::ยป This cannot replace a careful study of the O2k-Manual.
<br>
ย 
__TOC__
__TOC__
:::: Complementary to the '''[[O2k-Manual]]''', the O2k-Videosupport provides valuable assistance.
:::: This cannot replace a careful study of the O2k-Manual.
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[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/9TYfEAz96Lc]] [https://youtu.be/9TYfEAz96Lc A DL7 O2k ready to use] ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use] ย 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/H1NZbLI_fuQ]] [https://youtu.be/H1NZbLI_fuQ A1 DL7 OROBOROS flash drive and DatLab 7 installation] ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation] ย 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/0EfCReolzdk]] [https://youtu.be/0EfCReolzdk A2 DL7 Start DatLab7]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber] ย 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber] ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS] ย 
::::* '''O2k-Series H is delivered with the O2k-Chambers mounted'''. Therefore, mounting is only necessary after removing the glass chamber for a full cleaning service. This is required for regular [[MiPNet19.03 O2k-cleaning and ISS |cleaning the glass chamber]].
ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 '''A4''' O2k-chamber volume calibration]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
::::* '''O2k-Series H is delivered with volume-calibrated O2k-Chambers'''. Therefore, volume calibration is only necessary for specific stoppers of the [[O2k-TPP+ ISE-Module]], the [[O2k-pH ISE-Module]], and the [[O2k-NO Amp-Module]].
ย  ย 
ย  ย 
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber] ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber] ย 


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
ย  ย 
ย  ย 
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 B1a OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro B1b Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 B2 Cathode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 B3 Anode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA B4 Membrane mounting]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ B5 DL7 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]


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[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== DatLab 7.4 ==
<div class="mw-collapsible-content">
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.beย  How to perform an experiment with a SUIT DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== O2k-Mitochondrial preparations ==
<div class="mw-collapsible-content">
[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.beย  Fiber permeabilization]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== Q-Module (NextGen-O2k - DatLab 7) ==
<div class="mw-collapsible-content">
* '''Q-service'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.beย  Assembly of the reference electrode]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
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* ''' Q-experiments'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
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* ''' Cleaning Q-Sensors'''
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== Exclusive material for O2k-Workshop ==
<div class="mw-collapsible-content">
* '''O2k Quality Control'''
[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
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* '''O2k-Mitochondrial preparations'''
[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber
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* '''FluoRespirometry'''
[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly
[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor
[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4
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Revision as of 09:17, 17 October 2020


                  


O2k-Open Support

O2k-Videosupport


Template NextGen-O2k.jpg
O2k-Videosupport
ยป The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
ยป This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps



Click to expand or collaps

Exclusive material for O2k-Workshop


  • O2k Quality Control


PlayVideo.jpg O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration



  • O2k-Mitochondrial preparations


PlayVideo.jpg Addition of biological sample to the respirometer chamber



  • FluoRespirometry


PlayVideo.jpg Smart Fluo-Sensor assembly

PlayVideo.jpg Exchanging a filter set of the smart-fluo sensor

PlayVideo.jpg H2O2 flux analysis with DatLab 7.4