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Difference between revisions of "O2k-Videosupport"

From Bioblast
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[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s DL7 O2k ready to use]  
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]  


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s DL7 Oroboros flash drive and DatLab 7 installation]  
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]  


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 Chamber service Insert O2k Chamber]  
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]  


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 DL7 Insert OroboPOS]  
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]  


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 Chamber service O2k Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 '''A4''' O2k-chamber volume calibration]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 DL7 Start DatLab7]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
   
   


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[[File:Expand.png|right|45px |Click to expand or collaps]]
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
<div class="toccolours mw-collapsible">
== Exclusive material for O2k-Events (in progress) ==
== Exclusive material for O2k-Workshop ==
<div class="mw-collapsible-content">
<div class="mw-collapsible-content">


[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
=== O2k-Basic ===
<div class="mw-collapsible-content">
* '''Part I.I: OroboPOS service and O2k instrumental setup'''
---------




* '''Part I.II: O2k-Quality control'''
* '''O2k Quality Control'''


[[Image:PlayVideo.jpg|50px|link= ]] [ O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]


[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration


---------
---------




* '''Part II:I: Biological samples'''
* '''O2k-Mitochondrial preparations'''


[[Image:PlayVideo.jpg|50px|link= ]] [ Addition of biological sample to the respirometer chamber]


[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber


---------
---------




* '''Part II.II: Experimental design'''
* '''FluoRespirometry'''
 




[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly


---------
[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor
 
 
* '''Part II.III: Biological experiment and data analysis'''
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
=== O2k-Advance ===
<div class="mw-collapsible-content">


[[Image:PlayVideo.jpg|50px|link= ]] [ Smart Fluo-Sensor assembly]
[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4


[[Image:PlayVideo.jpg|50px|link= ]] [ Exchanging a filter set of the smart-fluo sensor]


</div>
</div>

Revision as of 10:17, 17 October 2020


                  

O2k-Open Support

O2k-Videosupport

Template NextGen-O2k.jpg
O2k-Videosupport
» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
» This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps

O2k-start (DatLab 7/Series H)

MiPNet22.11 O2k-FluoRespirometer manual MiPNet22.11 O2k-FluoRespirometer manual

PlayVideo.jpg O2k ready to use

PlayVideo.jpg A1 Oroboros flash drive and DatLab 7 installation

PlayVideo.jpg A2 Insert O2k Chamber

PlayVideo.jpg A3 Insert OroboPOS

PlayVideo.jpg A4 O2k-chamber volume calibration

PlayVideo.jpg A5 DL7 Start DatLab7



Click to expand or collaps

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start MiPNet19.18A O2k-Series G: Start

PlayVideo.jpg Insert O2k-Chamber

PlayVideo.jpg Insert OroboPOS

PlayVideo.jpg O2k-Chamber volume calibration

PlayVideo.jpg O2k ready to use


Click to expand or collaps

POS-service and Chamber-service (all series)


  • POS-service

MiPNet19.18B POS-service MiPNet19.18B POS-service

PlayVideo.jpg OroboPOS from Perspex housing

PlayVideo.jpg Disassembly of OroboPOS

PlayVideo.jpg Cathode cleaning

PlayVideo.jpg Anode cleaning

PlayVideo.jpg Membrane mounting

PlayVideo.jpg DL7 Insert OroboPOS


  • Chamber-service

PlayVideo.jpg Chamber service Insert O2k Chamber

PlayVideo.jpg Chamber service O2k Chamber volume calibration



Click to expand or collaps

DatLab 7.4

PlayVideo.jpg How to find a DL-Protocol (DLP)

PlayVideo.jpg How to perform an experiment with a SUIT DL-Protocol (DLP)

PlayVideo.jpg O2 flux analysis with DatLab 7.4

PlayVideo.jpg Export DL-Protocol User (*.DLPU)



Click to expand or collaps

O2k-Mitochondrial preparations


MiPNet17.02 PBI-Shredder manual MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers MiPNet17.03 Shredder vs Fibers


PlayVideo.jpg Shredder tissue homogenate

PlayVideo.jpg Fiber permeabilization



Click to expand or collaps

Q-Module (NextGen-O2k - DatLab 7)


  • Q-service


PlayVideo.jpg Assembly of the reference electrode

PlayVideo.jpg NG Polishing glassy carbon and platinum electrodes

PlayVideo.jpg NG O2k-Chamber volume calibration with Q-Sensor


  • Q-experiments

PlayVideo.jpg NG Q-experiments


  • Cleaning Q-Sensors


PlayVideo.jpg NG Cleaning after cyclic voltammetry

PlayVideo.jpg NG Cleaning after Q-experiments



Click to expand or collaps

Exclusive material for O2k-Workshop


  • O2k Quality Control


PlayVideo.jpg O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration


  • O2k-Mitochondrial preparations


PlayVideo.jpg Addition of biological sample to the respirometer chamber


  • FluoRespirometry


PlayVideo.jpg Smart Fluo-Sensor assembly

PlayVideo.jpg Exchanging a filter set of the smart-fluo sensor

PlayVideo.jpg H2O2 flux analysis with DatLab 7.4



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