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Difference between revisions of "O2k-Videosupport"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[Image:O2k-Manual.jpg|right|200px|link=http://wiki.oroboros.at/index.php/O2k-Manual |O2k-Manual]]
{{NextGen-O2k H2020-support}}
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
::ยป The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
::::ยป This cannot replace a careful study of the O2k-Manual.
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ย 
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== O2k-start (DatLab 7/Series H - I) ==
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[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]


:::: Complementary to the '''[[O2k-Manual]]''', the O2k-Videosupport provides valuable assistance.
=== '''FluoRespirometry''' ===


:::: This can't replace a careful study of the O2k-Manual.


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor]


== [[MiPNet19.18A O2k-Series G: Start]] ==
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== O2k-start (DatLab 6/Series G) ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber] ย 
[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
::::* ''New'': O2k-Series H is delivered with the O2k-Chambers mounted. Therefore, mounting is only necessary after removing the glass chamber for a full cleaning service. This is required for regular [[MiPNet19.03 O2k-cleaning and ISS |cleaning the glass chamber]].


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber] ย 


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]
::::* ''New'': O2k-Series H is delivered with volume-calibrated O2k-Chambers. Therefore, volume calibration is only necessary for specific stoppers of the [[O2k-TPP+ ISE-Module]], the [[O2k-pH ISE-Module]], and the [[O2k-NO Amp-Module]].


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]
ย 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
ย  ย 
ย  ย 
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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== POS-service and Chamber-service (all series) ==
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* '''POS-service'''
[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
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* ''' Chamber-service'''
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw Chamber service O2k Chamber volume calibration]
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== O2k Quality Control ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=onOIVpmOEoE ]] [https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
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== DatLab 7.4 ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.beย  How to perform an experiment with a SUIT DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
=== '''FluoRespirometry''' ===
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=_Fv0VSA_c14 ]] [https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
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== O2k-Mitochondrial preparations ==
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[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.beย  Fiber permeabilization]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GX_SvnVGXgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=NJj5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber]
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== Q-Module (NextGen-O2k - DatLab 7) ==
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* '''Q-service'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.beย  Assembly of the reference electrode]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
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* ''' Q-experiments'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
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* ''' Cleaning Q-Sensors'''
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]


== [[MiPNet19.18B POS-service]] ==
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Yso5KZUvefk]] [https://www.youtube.com/watch?v=Yso5KZUvefk B1 OroboPOS from Perspex housing]
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=WWm9bAr2hNQ]] [https://www.youtube.com/watch?v=WWm9bAr2hNQ B2 Disassembly of OroboPOS]
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GmAfRzpbHs8]] [https://www.youtube.com/watch?v=GmAfRzpbHs8 B3 Cathode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=855Q--AZmYo]] [https://www.youtube.com/watch?v=855Q--AZmYo B4 Anode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=KdSVajj0tSs]] [https://www.youtube.com/watch?v=KdSVajj0tSs B5 Membrane mounting]
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Revision as of 14:12, 8 August 2022


                  


O2k-Open Support

O2k-Videosupport


Template NextGen-O2k.jpg
O2k-Videosupport
ยป The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
ยป This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps


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