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Difference between revisions of "O2k-Videosupport"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[Image:O2k-Manual.jpg|right|200px|link=http://wiki.oroboros.at/index.php/O2k-Manual |O2k-Manual]]
{{NextGen-O2k H2020-support}}
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
::Β» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
::::Β» This cannot replace a careful study of the O2k-Manual.
<br>
Β 
__TOC__
__TOC__
:: Complementary to the '''[[O2k-Manual]]''', the O2k-Videosupport provides valuable assistance.
:: This cannot replace a careful study of the O2k-Manual.
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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== O2k-start (DatLab 7/Series H) ==
== O2k-start (DatLab 7/Series H - I) ==
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<div class="mw-collapsible-content">


[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/9TYfEAz96Lc]] [https://youtu.be/9TYfEAz96Lc A DL7 O2k ready to use] Β 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
Β 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation] Β 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/H1NZbLI_fuQ]] [https://youtu.be/H1NZbLI_fuQ A1 DL7 OROBOROS flash drive and DatLab 7 installation] Β 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber] Β 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/0EfCReolzdk]] [https://youtu.be/0EfCReolzdk A2 DL7 Start DatLab7]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS] Β 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]
::::* '''O2k-Series H is delivered with the O2k-Chambers mounted'''. Therefore, mounting is only necessary after removing the glass chamber for a full cleaning service. This is required for regular [[MiPNet19.03 O2k-cleaning and ISS |cleaning the glass chamber]].


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
::::* '''O2k-Series H is delivered with volume-calibrated O2k-Chambers'''. Therefore, volume calibration is only necessary for specific stoppers of the [[O2k-TPP+ ISE-Module]], the [[O2k-pH ISE-Module]], and the [[O2k-NO Amp-Module]].
Β  Β 
Β  Β 
=== '''FluoRespirometry''' ===
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor]
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber] Β 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber] Β 


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
Β  Β 
Β  Β 
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 B1a OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
Β 
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
Β 
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro B1b Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 B2 Cathode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 B3 Anode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA B4 Membrane mounting]


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ B5 DL7 Insert OroboPOS]


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[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber] Β 
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber] Β 


[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw Chamber service O2k Chamber volume calibration]




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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== O2k Quality Control ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=onOIVpmOEoE ]] [https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== DatLab 7.4 ==
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[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.beΒ  How to perform an experiment with a SUIT DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
=== '''FluoRespirometry''' ===
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=_Fv0VSA_c14 ]] [https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== O2k-Mitochondrial preparations ==
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[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.beΒ  Fiber permeabilization]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GX_SvnVGXgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=NJj5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber]
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[[File:Expand.png|right|45px |Click to expand or collaps]]
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== Q-Module (NextGen-O2k - DatLab 7) ==
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* '''Q-service'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.beΒ  Assembly of the reference electrode]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
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* ''' Q-experiments'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
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* ''' Cleaning Q-Sensors'''
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
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Revision as of 14:12, 8 August 2022


                  


O2k-Open Support

O2k-Videosupport


Template NextGen-O2k.jpg
O2k-Videosupport
Β» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
Β» This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps


Click to expand or collaps