Difference between revisions of "O2k-Videosupport"

Revision as of 15:12, 8 August 2022

O2k-Videosupport

» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.

» This cannot replace a careful study of the O2k-Manual.

O2k-start (DatLab 7/Series H - I)

MiPNet22.11 O2k-FluoRespirometer manual

O2k ready to use

A1 Oroboros flash drive and DatLab 7 installation

A2 Insert O2k Chamber

A3 Insert OroboPOS

A4 O2k-chamber volume calibration

A5 DL7 Start DatLab7

FluoRespirometry

Smart Fluo-Sensor assembly

Exchanging a filter set of the Smart Fluo-Sensor

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start

Insert O2k-Chamber

Insert OroboPOS

O2k-Chamber volume calibration

O2k ready to use

POS-service and Chamber-service (all series)

POS-service

MiPNet19.18B POS-service

OroboPOS from Perspex housing

Disassembly of OroboPOS

Chamber-service

Chamber service Insert O2k Chamber

Chamber service O2k Chamber volume calibration

O2k Quality Control

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

DatLab 7.4

How to find a DL-Protocol (DLP)

How to perform an experiment with a SUIT DL-Protocol (DLP)

O₂ flux analysis with DatLab 7.4

Export DL-Protocol User (*.DLPU)

FluoRespirometry

H₂O₂ flux analysis with DatLab 7.4

O2k-Mitochondrial preparations

MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers

Shredder tissue homogenate

Fiber permeabilization

Addition of biological sample to the respirometer chamber

Insertion of the Sample Holder into the 2.0 mL O2k-chamber

Q-Module (NextGen-O2k - DatLab 7)

Q-service

Assembly of the reference electrode

NG Polishing glassy carbon and platinum electrodes

NG O2k-Chamber volume calibration with Q-Sensor

Q-experiments

NG Q-experiments

Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry

NG Cleaning after Q-experiments

@@ Line 14: / Line 14: @@
 [[File:Expand.png|right|45px |Click to expand or collaps]]
 <div class="toccolours mw-collapsible">
-== O2k-start (DatLab 7/Series H) ==
+== O2k-start (DatLab 7/Series H - I) ==
 <div class="mw-collapsible-content">
 [[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s DL7 O2k ready to use]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s DL7 Oroboros flash drive and DatLab 7 installation]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 Chamber service Insert O2k Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 DL7 Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 Chamber service O2k Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 DL7 Start DatLab7]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
+=== '''FluoRespirometry''' ===
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor]
 </div>
@@ Line 77: / Line 84: @@
 [[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
 ---------
@@ Line 86: / Line 95: @@
 [[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw Chamber service O2k Chamber volume calibration]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== O2k Quality Control ==
+<div class="mw-collapsible-content">
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=onOIVpmOEoE ]] [https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
 </div>
 </div>
@@ Line 106: / Line 125: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
+=== '''FluoRespirometry''' ===
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=_Fv0VSA_c14 ]] [https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
 </div>
@@ Line 128: / Line 150: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GX_SvnVGXgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=NJj5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber]
 </div>
@@ Line 169: / Line 194: @@
 </div>
+<br/>
+</div>
+</div>
 <br/>