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Difference between revisions of "Octanoylcarnitine"

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<span  style="color:#8B008B">  '''Caution:''' Chemicals stored in the fridge  or freezer should be  allowed to reach room temperature before  opening.</span>


::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O or DMSO, can differ from batch to batch - check Certificate of Analysis):


::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O):
::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O or DMSO.  
 
::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O.  
::::# Divide into 0.2 mL portions.
::::# Divide into 0.2 mL portions.
::::# Store at -20 °C.
::::# Store at -20 °C.

Revision as of 14:00, 21 November 2019


high-resolution terminology - matching measurements at high-resolution


Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf


MitoPedia topics: Substrate and metabolite 

» Fatty acid oxidation
» Carnitine O-octanoyltransferase

Application in HRR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.


Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
  1. Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H2O or DMSO.
  2. Divide into 0.2 mL portions.
  3. Store at -20 °C.
O2k manual titrations into 2 mL O2k-chamber
  • Titration volume: 10 µL using a 25 µL syringe.
  • Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.