Difference between revisions of "Oligomycin"
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'''Preparation of | '''Preparation of 4 mg/ml (5 mM) stock solution''' (dissolved in 99.9% Ethanol): | ||
::1) weigh 4 mg of Oligomycin directly into a 2 ml glass vial | ::1) weigh 4 mg of Oligomycin directly into a 2 ml glass vial and dissolve in 1 ml Ethanol | ||
::2) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | |||
:: | ::3) store in glass vials (recommendation) at -20 °C | ||
:: | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
::* Titration volume: 1 µl using a 10 µl syringe | ::* Titration volume: 1 µl using a 10 µl syringe | ||
::* Final conc. in 2 ml O2k-chamber: 2 µg/ml | ::* Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM) |
Revision as of 10:13, 7 December 2010
Description
Oligomycin is an inhibitor of ATP synthase.
Abbreviation: Omy
Reference: MiPNet09.12
Labels:
Enzyme: Complex V; ATP Synthase"Complex V; ATP Synthase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes, mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C: FW = 800
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 4 mg/ml (5 mM) stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 4 mg of Oligomycin directly into a 2 ml glass vial and dissolve in 1 ml Ethanol
- 2) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM)