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Difference between revisions of "Oligomycin"

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|description='''Oligomycin''' (Omy) is an inhibitor of [[ATP synthase]] by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a [[LEAK]] state of respiration.
|description='''Oligomycin''' (Omy) is an inhibitor of [[ATP synthase]] by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a [[LEAK]] state of respiration.
|info=[[MiPNet09.12]]
|info=[[MiPNet09.12]]
|type=Inhibitor PS
}}
{{MitoPedia concepts
|mitopedia concept=Respiratory state
|type=Inhibitor PS
|type=Inhibitor PS
}}
}}
{{MitoPedia methods|type=Inhibitor PS
{{MitoPedia methods|type=Inhibitor PS
}}
{{MitoPedia O2k and high-resolution respirometry|type=Inhibitor PS
}}
}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Respiratory state, Inhibitor
|mitopedia topic=Inhibitor
|type=Inhibitor PS
|type=Inhibitor PS
}}
}}

Revision as of 14:02, 8 February 2016


high-resolution terminology - matching measurements at high-resolution


Oligomycin

Description

Oligomycin (Omy) is an inhibitor of ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a LEAK state of respiration.

Abbreviation: Omy

Reference: MiPNet09.12


MitoPedia concepts: Respiratory state 





MitoPedia topics: Inhibitor 

Application in HRR

Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes; mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg; store at -20 °C; FW = 800.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 5 mM storage and stock solution (dissolved in 99.9% ethanol):

1) Weigh 4 mg oligomycin into a 2 ml glass vial.
2) Dissolve in 1 ml ethanol.
3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution.
4) Store in glass vials (recommendation) at -20 °C.


Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
  • Final concentration: 2 µg/ml (2.5 µM).


Problems with the use of oligomycin

  • Several researchers have reported problems associated with the use of oligomycin which appear primarily related to difficulties with getting rid of the inhibitor after application in a respirometry chamber. In our hands following rigouros washing procedures generally helps to avoid such problems: for more details, see MiPNet19.03_O2k-cleaning_and_ISS. Solutions suggested by our users in the O2k-Network Discussion forum can be found on the Discussion page
  • Oligomycin has been found ineffective as inhibitor of the ATP synthase in various strains of yeast, a problem we were made aware of by Gennaro Agrimi and which is briefly described and discussed here.


>>Discussion