Difference between revisions of "Oligomycin"
| Line 21: | Line 21: | ||
::1) Weigh 4 mg of Oligomycin into a 2 ml glass vial. | ::1) Weigh 4 mg of Oligomycin into a 2 ml glass vial. | ||
::2) Dissolve in 1 ml | ::2) Dissolve in 1 ml ethanol. | ||
::3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution. | ::3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution. | ||
::4) Store in glass vials (recommendation) at -20 °C. | ::4) Store in glass vials (recommendation) at -20 °C. | ||
| Line 28: | Line 28: | ||
'''Oxygraph-2k manual titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k manual titrations:''' [[MiPNet09.12]] | ||
::* Titration volume: 1 µl using a 10 µl | ::* Titration volume: 1 µl using a 10 µl syringe. | ||
::* Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM). | ::* Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM). | ||
[[Talk:Oligomycin|>>Discussion]] | [[Talk:Oligomycin|>>Discussion]] | ||
Revision as of 16:36, 20 October 2013
- high-resolution terminology - matching measurements at high-resolution
Oligomycin
Description
Oligomycin (Omy) is an inhibitor of ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a LEAK state of respiration.
Abbreviation: Omy
Reference: MiPNet09.12
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property., Inhibitor
Application in HRR
Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes; mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg; store at -20 °C; FW = 800.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 4 mg of Oligomycin into a 2 ml glass vial.
- 2) Dissolve in 1 ml ethanol.
- 3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- 4) Store in glass vials (recommendation) at -20 °C.
Oxygraph-2k manual titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe.
- Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM).
>>Discussion
