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Difference between revisions of "Oroboros Ecosystem Agenda 2020 Archive"

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Ā  2020 Week 39
== Last week ==
Ā  2020 Week 48


* '''Sep-21 Mo''' - [[ESCI 2020 Virtual Event]]
* '''Nov-23 Mo''' Ā 
:::: [[File:Oegmbt.png|100px|link=https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Graz_AT|ƖGMBT Graz AT]] <big>'''[[12th ƖGMBT Annual Meeting 2020 Virtual Event]]'''</big> Sep 21-23
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
|'''Cell culture medium and dithionite titrations ā€“ a bad combination for an instrumental O<sub>2</sub> background test?'''<br>
Cell culture media are not ideal for instrumental O<sub>2</sub> background test when using dithionite titrations because side reactions affecting the O<sub>2</sub> concentration and thus interfering with the test. Instead of cell culture media, use [[MiR05]], [[MiR06]] or a strongly buffered alkaline phosphate solution. <br>
-Ā»[[MiPNet14.06 Instrumental O2 background]]Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cecatto Cristiane]]Ā«
|}
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* '''Nov-24 Tu'''
:::: [[ISCaM 2020 Virtual Event]]


- World Alzheimer's Day
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* '''Nov-25 We'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Olive polyphenols prove promising in the protection against mitochondrial dysfunction due to Alzheimer's disease and brain aging'''<br>
|'''Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.'''<br>
:from the O2k-Network Ā»[[DE Giessen Eckert GP]]Ā«
: from the O2k-Network Ā»[[CA Guelph Holloway GP]]Ā«
*Grewal R, Reutzel M, Dilberger B, Hein H, Zotzel J, Marx S, Tretzel J, Sarafeddinov A, Fuchs C, Eckert GP (2020) Purified oleocanthal and ligstroside protect against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing. Exp Neurol 328:113248. [[Grewal 2020 Exp Neurol|Ā»Bioblast linkĀ«]]
*Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. [[Politis-Barber 2020 Am J Physiol Endocrinol Metab|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Nov-26 Th'''


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* '''Nov-27 Fr'''
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''''HSP60'' knockdown and its effect on mitochondriaā€™s role in pancreatic ductal adenocarcinoma''' <br>


| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
* Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161.Ā  [[Zhou 2018 Cell Death Dis|Ā»Bioblast linkĀ«]]
|'''Resistance training effects in Alzheimerā€™s disease mouse model''' <br>
: from the O2k-Network Ā»[[US FL Boca Raton Khamoui AV]]Ā« <br>
* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Dis 2020:Article ID 5919501.Ā  [[Pena 2020 Int J Chronic Dis|Ā»Bioblast linkĀ«]]
|}
|}
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Ā 
* '''Sep-22 Tu''' - [[12th ƖGMBT Annual Meeting 2020 Virtual Event]] , [[ESCI 2020 Virtual Event]]
== Nov 2020 ==
::::
Ā 
[[File:Expand.png|right|35px |Click to expand or collaps]]
Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā 
2020 Week 45
* '''Nov-02 Mo''' Ā 
:{|
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Did you know the reverse electron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using [[Amplex UltraRed]] assay?'''<br>
|''' How can you measure mitochondrial membrane potential with O2k-Fluorometry? '''<br>
The reverse electron flow, also called reverse electron transfer (RET), happens when electrons are transferred from Complex II to the Q-junction and then back to Complex I at high mt-membrane potential. This phenomenon results in high hydrogen peroxide production in some mitochondrial preparations. The SUIT-026 protocol was designed to investigate this effect.Ā  <br>
The extrinsic fluorophores safranin, TMRM or Rhodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations. <br>
- Ā»'''[[SUIT-026]]'''Ā« <br>
- Ā»'''[[Safranin]]'''Ā«, Ā»'''[[TMRM]]'''Ā«, Ā»'''[[Rhodamine 123]]'''Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā« Ā 
- communicated by Ā»[[Komlodi Timea]]Ā«, Ā»[[Iglesias-Gonzalez Javier]]Ā« and Ā»[[Cecatto Cristiane]]Ā«
|}
|}
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* '''Sep-23 We''' - [[12th ƖGMBT Annual Meeting 2020 Virtual Event]] , [[ESCI 2020 Virtual Event]]


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* '''Sep-24 Th''' - [[ESCI 2020 Virtual Event]]
* '''Nov-03 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ƖGMBT Life Science Tuesdays]]
::::* 18:00 - Houska Awards 2020 Vienna - [https://bcgruppe.at/houskapreis/ Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien]


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* '''Sep-25 Fr''' -[[ESCI 2020 Virtual Event]]
* '''Nov-04 We'''
2020 Week 38
* '''Sep-14 Mo'''
:{|
| width="100" align="center" | [[Image:MitoGlobal.jpg|45px|link=https://www.mitoglobal.org/index.php/MitoGlobal_Events|MitoGlobal Events]]
| '''World Mitochondrial Disease Week''' ā€“ Sep 13-19
World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.


See what is happening near you - Ā»[https://mitochondrialdiseaseweek.org/events/ https://mitochondrialdiseaseweek.org/events/]
|}
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* '''Sep-15 Tu''' - World Lymphoma Awareness Day
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''OXPHOS as a potential therapeutic target in classical Hodgkin lymphoma'''<br>
|'''Six-month caloric restriction causes tissue-specific bioenergetic changes in mitochondria isolated from rat heart and skeletal muscle'''<br>
:from the O2k-Network Ā»[[DE Frankfurt Droese S]]Ā«, Ā»[[DE Frankfurt Wittig I]]Ā« and Ā»[[NL Nijmegen Brandt U]]Ā«
:from the O2k-Network Ā»[[BR Sao Paulo Kowaltowski AJ]]Ā«
*Birkenmeier K, Droese S, Wittig I, Winkelmann R, KƤfer V, Doering C, Hartmann S, Wenz T, Reichert AS, Brandt U, Hansmann ML (2016) Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma are highly dependent on oxidative phosphorylation. Int J Cancer 138:2231-46. [[Birkenmeier 2016 Int J Cancer|Ā»Bioblast linkĀ«]]
*Serna JDC, Caldeira da Silva CC, Kowaltowski AJ (2020) Functional changes induced by caloric restriction in cardiac and skeletal muscle mitochondria. J Bioenerg Biomembr 52:269-77.[[Serna 2020 J Bioenerg Biomembr|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Sep-16 We'''
* '''Nov-05 Th'''
:{|


| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
----
|''' Why are permeabilized muscle fibers (pfi) a poor sample type for studying reactive oxygen species (ROS) production? '''<br>
* '''Nov-06 Fr'''
In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation. However, the high oxygen pressures used may artificially increase ROS, including H<sub>2</sub>O<sub>2</sub> production, making pfi a less optimal model for ROS production studies. <br>
Ā 
- Ā»'''[[Permeabilized_muscle_fibers#Oxygen_dependence_of_ROS_production_-_are_permeabilized_fibers_a_valid_model.3F|Oxygen dependence of ROS production in pfi]]'''Ā« <br>
:::: Registration deadline for: [[Integrative Physiology of Exercise 2020 Virtual Event]]
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā«
2020 Week 46
Ā 
* '''Nov-09 Mo''' - <big>'''[[Integrative Physiology of Exercise 2020 Virtual Event]]'''</big> Nov 9- 13
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
|''' Have you observed a high signal at zero oxygen?'''<br>
A high signal at zero oxygen might be due to a technical issue with the O2k-Main Unit, the OroboPOS-Connector, the OroboPOS or a bad electrical connection between these components. The high zero current test helps you to locate the cause. Ā 
<br>
- Ā»'''[[High signal at zero oxygen]]'''Ā« <br>
- communicated by Ā»[[Schmitt Sabine]]Ā« and Ā»[[Komlodi Timea]]Ā«
|}
|}
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* '''Sep-17 Th'''
* '''Nov-10 Tu''' Ā 
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
|''' Did you know that you can measure the respiratory activity available for phosphorylation of ADP to ATP in living cells?'''<br>
SUIT protocols from the [[SUIT-003]] family will guide you through the titrations needed to analyze [[free ROUTINE activity]].<br>
- Ā»'''[[SUIT-003 O2 ce D009]]'''Ā« and '''Ā»[[SUIT-003 O2 ce-pce D020]]'''Ā« <br>
- communicated by Ā»[[Cardoso Luiza]]Ā« and Ā»[[Komlodi Timea]]Ā«
|}


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* '''Sep-18 Fr'''
* '''Nov-11 We'''


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* '''Sep-19 Sa''' Ā 
* '''Nov-12 Th'''
Ā 
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* '''Sep-20 So'''
* '''Nov-13 Fr'''
:::: [[File:ESCI.JPG|100px|link=https://wiki.oroboros.at/index.php/ESCI_2020_Virtual_Event]] <big>'''[[ESCI 2020 Virtual Event]]'''</big> Sep 20-30
Ā 
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* '''Nov-14 Sa''' - World Diabetes Day


2020 Week 37
* '''Sep-07 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''Why it is necessary to perform multiple H<sub>2</sub>O<sub>2</sub> calibrations when using the [[Amplex UltraRed]] (AmR) assay?'''<br>
|'''Gastric bypass surgery improves diabetic nephropathy by reducing NADPH production via restoration of AMPK activity in type 1 diabetic rats'''<br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H<sub>2</sub>O<sub>2</sub> calibration before sample addition, after sample addition and multiple times during the experiment. <br>
:from the O2k-Network Ā»[[CN Chongqing Zhu Z]]Ā«
- Ā»[[Komlodi 2018 Methods Mol Biol]]Ā« <br>
*Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207. [[Wei 2020 Cell Rep|Ā»Bioblast linkĀ«]]
|}
Ā 
2020 Week 47


The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H<sub>2</sub>O<sub>2</sub> calibrations implemented. <br>
* '''Nov-16 Mo''' Ā 
- Ā» [https://www.bioblast.at/index.php/SUITbrowser SUITbrowser]Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā«
|}
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* '''Sep-08 Tu'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung'''<br>
|'''Mitochondrial function is impaired in human iPSC-derived astrocytes infected with Zika virus.'''<br>
:from the O2k-Network Ā»[[DE Giessen Weissmann N]]Ā«, Ā»[[ FI Tampere Dufour E]]Ā«, Ā»[[FI Helsinki Jacobs HT]]Ā« and Ā»[[DE Jena Szibor M]]Ā«
:from the O2k-Network Ā»[[BR Rio de Janeiro Galina A]]Ā« and Ā»[[BR Rio de Janeiro Institute Biomedical Chemistry]]Ā« Ā 
*Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. [[Sommer 2020 Sci Adv|Ā»Bioblast linkĀ«]]
*Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. [[Ledur 2020 Sci Rep|Ā»Bioblast linkĀ«]]
|}
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* '''Sep-09 We'''
* '''Nov-17 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ƖGMBT Life Science Tuesdays]]
Ā 
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* '''Nov-18 We''' Ā 


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* '''Sep-10 Th'''
* '''Nov-19 Th''' Ā 
::::Ā» <big>'''[[2020 PaduaMuscleDays Padua IT]]'''</big> / [[MiPNet25.04 IOC146 Padua IT|<big>'''IOC Padua IT'''</big>]]
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br>
| '''What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?'''<br>
OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P<sub>i</sub>, fuel substrates and O<sub>2</sub>. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of [[excess E-P capacity]] due to kinetic limitations of the phosphorylation system. <br>
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.<br>
- Ā»'''[[BEC_2020.1_doi10.26124bec2020-0001.v1|BEC: Mitochondrial physiology]]'''Ā« - Table 3 and several figures. <br>
- Ā»[[Permeabilized_muscle_fibers#Chemicals_and_media |Optimum ADP concentration in pfi]]Ā«Ā  <br>
- communicated by Ā» [[Cecatto Cristiane]] Ā« and Ā» [[Komlodi Timea]] Ā«
- Ā» [[Komlodi Timea]]Ā« and Ā» [[Doerrier Carolina]]Ā«
|}
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* '''Nov-20 Fr''' Ā 
:::: [[2020 PaduaMuscleDays Padua IT]]
Ā 
:{|
| width="110" align="center" |Ā  [[File:O2k-Workshops.jpg|left|80px|O2k-Events|link=O2k-Events]]
| '''[[Doerrier Carolina|Dr. Carolina Doerrier]] and [[Gnaiger Erich|Dr. Erich Gnaiger]] will be presenting at the virtual [[2020 PaduaMuscleDays Padua IT]]'''<br>
:* 09:30 - Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers - Ā»[[MiPNet25.04 IOC146 Padua IT|'''IOC Padua IT''']]Ā« <br>
:* 15:40 - Body mass excess, muscle mass, obesity and mitochondrial fitness
|}
|}


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* '''Sep-11 Fr'''


2020 Week 36
* '''Nov-21 Sa''' Ā 
* '''Aug-31 Mo''' Ā 
:::: [[2020 PaduaMuscleDays Padua IT]]
</div>
</div>
== Oct 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]


<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
* '''Oct-01 Th'''
:::: Abstract submission deadline for: [[Integrative Physiology of Exercise 2020 Virtual Event]]
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* '''Sep-01 Tu'''
* '''Oct-02 Fr''' Ā 
Ā 
Ā 
2020 Week 41
* '''Oct-05 Mo''' [[File:O2kHandOver 20201002.jpg|right|200px]]
:{|
| width="100" align="center" |[[Image:FFG-logo.png|70px|FFG]]
|'''Dr. Markus Keller and his team have now received the O2ks for their measurements in the FFG-funded TissueModels project!'''<br>
The project aims to establish physiological models for specific tissue types. Oroboros Instruments is a consortium partner, providing infrastructure and expertise.
Ā 
- Read more Ā»'''[[TissueModels]]'''Ā«
|}


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* '''Oct-06 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ƖGMBT Life Science Tuesdays]]
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction'''<br>
|'''Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?'''<br>
:from the O2k-Network Ā»[[US PA Philadelphia Kilbaugh T]]Ā«, Ā»[[US PA Philadelphia Jang DH]]Ā« and Ā»[[SE Lund Elmer E]]Ā«
:from the O2k-Network Ā»[[BR Sao Paulo Kowaltowski AJ]]Ā« Ā 
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|Ā»Bioblast linkĀ«]]
*Menezes-Filho SL, Amigo I, LuĆ©vano-MartĆ­nez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. [[Menezes-Filho 2018 Biochim Biophys Acta Bioenerg|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Sep-02 We'''
* '''Oct-07 We'''
Ā 
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Background H<sub>2</sub>O<sub>2</sub> calibration: what is it and why perform it when using Amplex UltraRed assay?'''<br>
Background H<sub>2</sub>O<sub>2</sub> calibration refers to a set of H<sub>2</sub>O<sub>2</sub> titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay. <br>
- Ā»'''[[Amplex_UltraRed#Calibration_with_H2O2|Background H<sub>2</sub>O<sub>2</sub> calibration]]'''Ā«Ā  <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Schmitt Sabine]]Ā«
|}


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* '''Sep-03 Th'''
* '''Oct-08 Th'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Childhood maltreatment is associated with increased mitochondrial bioenergetic function of immune cells in mothers, but not in their newborns'''<br>
:from the O2k-Network Ā»[[DE Ulm Gumpp A]]Ā«
*Gumpp AM, Boeck C, Behnke A, Bach AM, Ramo-FernĆ”ndez L, Welz T, GĆ¼ndel H, Kolassa IT, Karabatsiakis A (2020) Childhood maltreatment is associated with changes in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84.Ā  [[Gumpp 2020 Proc Natl Acad Sci U S A|Ā»Bioblast linkĀ«]]
|}


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* '''Sep-04 Fr'''
* '''Oct-09 Fr''' Ā 
:::: Ā»<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>Ā«


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* '''Sep-05 Sa''' Ā 
Ā 
2020 Week 42
* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
- Ā»[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] Ā« selectĀ  ā€œAmR calibrationā€Ā  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Schmitt Sabine]]Ā«
|}
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* '''Sep-06 So''' Ā 
* '''Oct-13 Tu'''
::::* Abstract submission deadline - Ā»[[ESSR 2020 Innsbruck AT]]Ā«
:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] Ā»<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>Ā«
== Aug 2020 ==


2020 Week 35
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* '''Oct-14 We'''


* '''Aug-24 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Can NADH be used as an externally added substrate to obtain the [[NADH electron transfer-pathway state]] in [[Mitochondrial_respiration |mitochondrial respiration]]? '''<br>
|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
No, in most types of intact mitochondria NADH cannot pass the [[mitochondrial inner membrane]] (mtIM). Yeast mitochondria are an exception. <br>
:from the O2k-Network Ā»[[US VA Richmond Lesnefsky EJ]]Ā« <br>
- Ā»[[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide]]Ā« and Ā»[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]Ā«
*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Aug-25 Tu'''
* '''Oct-15 Th'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Low-intensity exercise increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults'''<br>
|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?Ā  '''<br>
:from the O2k-Network Ā»[[LV Riga Makrecka-Kuka M]]Ā«
The O2k-sV-ModuleĀ  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.Ā  <br>
*Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. ā€” [[Liepinsh 2020 Physiol Rep|Ā»Bioblast linkĀ«]]
- Ā»'''[[O2k-sV-Module]]'''Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Schmitt Sabine]]Ā«
|}
|}
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* '''Aug-26 We''' Ā 
* '''Oct-16 Fr'''


:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Ā 
| '''Non-mitochondrial respiration or [[residual oxygen consumption]] (''Rox'')? ā€” Concept-driven constructive terminology frames our perception.''' Ā 
| '''Explore mitochondrial pathways in[[PBMC]] and [[Platelet|platelets]]'''<br>
After inhibition of electron-transfer (ET) pathways or in the absence of fuel substrates, both ''mitochondrial'' non-ET reactions and ''non-mitochondrial'' reactions contribute to ''residual oxygen consumption'' in mt-preparations and living cells.Ā  <br>
The following SUIT protocols are specifically optimized for PBMC and platelets <br>
- Ā»'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''Ā« ā€” Table 1: ''Rox'' induced by non-ET pathway oxidation reactions
- Ā»'''[[SUIT-001 O2 ce-pce D004]]'''Ā« and Ā»'''[[SUIT-002 O2 ce-pce D007a]]'''Ā« <br>
- communicated by Ā»[[Cecatto Cristiane]]Ā« and Ā»[[Garcia-Souza Luiz F]]Ā« Ā 
|}
|}


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* '''Aug-27 Th''' Ā 
* '''Oct-17 Sa'''


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* '''Aug-28 Fr'''
2020 Week 34
* '''Aug-17 Mo'''Ā 


* '''Aug-18 Tu'''
* '''Oct-18 Su'''
:::: Ā»<big>'''[[ISCaM 2020 Virtual Event]]'''</big>Ā«
Ā  2020 Week 43


* '''Oct-19 Mo'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Mitochondrial respiration in PBMCs from patients with major depression'''<br>
|'''Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?'''<br>
:from the O2k-Network Ā»[[DE Ulm Karabatsiakis A]]Ā« and Ā»[[DE Ulm Radermacher P]]Ā«
Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 ĀµM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.<br>
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |Ā»Bioblast linkĀ«]]
- You can read more about it in the following publication: Ā»[[Krumschnabel 2014 Methods Enzymol]]Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cecatto Cristiane]]Ā«
|}
|}
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* '''Aug-19 We'''
* '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ƖGMBT Life Science Tuesdays]]


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* '''Aug-20 Th'''
* '''Oct-21 We'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br>
|'''Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies '''<br>
<br>
:from the O2k-Network Ā»[[US NC Winston-Salem Molina AJA]]Ā«
- Ā»'''[[Q-Module]]'''Ā«
*Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.[[Tyrrell 2016 Redox Biol|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Aug-21 Fr'''
2020 Week 33


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:{|
* '''Aug-10 Mo''' Ā 
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Human [[PBMC]] and [[Platelet|Platelets]] - samples for high-resolution respirometry obtained by minimally invasive procedures.'''<br>
We compared isolation procedures for blood cells.<br>
- Ā»'''[[MiPNet21.17_BloodCellsIsolation|MiPNet21.17 Blood Cells Isolation]]'''Ā«Ā  <br>
- communicated by Ā»[[Cecatto Cristiane]]Ā« and Ā»[[Garcia-Souza Luiz F]]Ā«
|}


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* '''Aug-11 Tu'''
* '''Oct-22 Th'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
Ā 
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br>
| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
:from the O2k-Network Ā»[[DE Bremerhaven Mark FC]]Ā«
|''' LEAK respiration relates to intrinsic uncoupling.Ā  '''<br>
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |Ā»Bioblast linkĀ«]]
Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.Ā  <br>
- Ā»'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''Ā« - Table 2. Terms on respiratory coupling and uncouplingĀ  <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cecatto Cristiane]]Ā« and Ā»[[Gnaiger Erich]]Ā«
|}
|}
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* '''Aug-12 We'''
* '''Oct-23 Fr'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
: from the O2k-Network Ā»[[AT Innsbruck Oroboros]]Ā« and Ā»[[AT Innsbruck Gnaiger E]]Ā«<br>
* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.Ā  [[Krumschnabel 2014 Methods Enzymol|Ā»Bioblast linkĀ«]]
|}
2020 Week 44
* '''Oct-26 Mo''' Ā 
:{|
:{|
| width="100" align="center" |[[File:MitoFit Preprint Arch pdf.png|100px|link=https://wiki.oroboros.at/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf |MitoFit pdf]]
| width="110" align="center" | [[File:TRANSMIT Acronym.jpg|left|70px|TRANSMIT|link=TRANSMIT]]
|'''Some terms in ā€œMitochondrial physiologyā€ ''Bioenergetics Communications'' 2020.1 (States and rates) did not look familiar ā€• what is the elementary unit [x]?'''
| Ā  Ā  '''"TRANSMIT: THE ROAD TO MEET THE MITOS"''' ā€“ the interactive [[TRANSMIT]] booklet for children is available for download in multiple languagesĀ  <br>
- Ā»https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/ <br>
|}


Fundamental answers, a critical discussion on the SI unit for ''count'', and the meaning of Euclidā€™s unit and Euclidā€™s number are presented in an unusual ā€˜'''canonical review'''ā€™:
:{|


* Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 [https://www.mitofit.org/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf doi:10.26124/mitofit:200004]. - [[Gnaiger_MitoFit_Preprint_Arch_2020.4 |'''Ā»Bioblast linkĀ«''']]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Did you know that oxygen flux can be converted to ''flux control ratios'' (''FCRs'') directly in DatLab?'''<br>
Use this function to easily plot your experimental traces as ''FCRs'', or to analyze your data.
<br>
- Ā»'''[[Flux_control_ratio#DatLab|''FCR'' in DatLab]]'''Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cecatto Cristiane]]Ā«
|}
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* '''Oct-27 Tu'''
:{|
| width="100" align="center" |[[Image:O2k-Network.png|left|70px|O2k-Network|link=O2k-Network]][[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
| '''The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab''' Ā 
:from the O2k-Network Ā»[[CH Lausanne Place N]]Ā« <br>
*Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. [[Burtscher 2020 iScience|Ā»Bioblast linkĀ«]]
|}
|}
<br />


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* '''Oct-28 We'''
:{|
:{|
Ā 
| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]
|''' Flow or flux - what is the difference? '''<br>
|Join the discussion on the MitoEAGLE Consortium Communication: ā€œMitochondrial physiologyā€ ''Bioenergetics Communications'' 2020.1
Rate can be expressed both as flow (''I'') and flux (''J''). Flow is used if the sample ''X'' can be quantified as [[count]] (''N<sub>X</sub>''; the number of cells, organisms), and changes proportionally with the size of ''X''. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.Ā  <br>
Ā 
- Ā»'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''Ā« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalarĀ  <br>
Ā» [https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1#On_a_Comment_in_Nature_Metabolism '''On a Comment in ''Nature Metabolism'''''] Ā«
- communicated by Ā»[[Cecatto Cristiane]]Ā« and Ā»[[Komlodi Timea]]Ā« and Ā»[[Gnaiger Erich]]Ā«
|}
|}
Ā 
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* '''Aug-13 Th'''
* '''Oct-29 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| Photosynthesis efficiency is reduced by 25 % in plants and algae due the metabolic process called photorespiration.Ā  <br>
|''' Would you like to monitor O<sub>2</sub> flux in response to light?Ā  '''<br>
- Ā»'''[[Photorespiration]]'''Ā«
Read about the PB-Module, the new module in the NextGen-O2k that will enable experiments in PhotoBiology! <br>
- Ā»'''[[PB-Module]]'''Ā«, Ā»'''[[PhotoBiology]]'''Ā«Ā  <br>
- communicated by Ā»[[Huete-Ortega Maria]]Ā« and Ā»[[Schmitt Sabine]]Ā« and Ā»[[Tindle-Solomon Lisa]]Ā«
|}
|}
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* '''Aug-14 Fr'''
* '''Oct-30 Fr'''
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|''' Ā  Ā  Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br>
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br>
: from the O2k-Network Ā»[[NZ Auckland Hickey AJ]]Ā« <br>
: from the O2k-Network Ā»[[HU Budapest Chinopoulos C]]Ā« <br>
* Ā  Ā  Ā  Ā  Ā  Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|Ā»Bioblast linkĀ«]]
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|Ā»Bioblast linkĀ«]]
|}
|}
Ā  2020 Week 32
Ā 
* '''Aug-03 Mo''' Ā 
</div>
</div>
Ā 
== Sep 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]
Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā 
Ā  2020 Week 36
Ā 
* '''Sep-01 Tu'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br>
|'''A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction'''<br>
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br>
:from the O2k-Network Ā»[[US PA Philadelphia Kilbaugh T]]Ā«, Ā»[[US PA Philadelphia Jang DH]]Ā« and Ā»[[SE Lund Elmer E]]Ā«
- Ā» [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]Ā«
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Aug-04 Tu'''
* '''Sep-02 We'''


:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br>
:from the O2k-Network Ā»[[US PA Pittsburgh Jurczak MJ]]Ā«
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|Ā»Bioblast linkĀ«]]
|}


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* '''Aug-05 We'''
* '''Sep-03 Th'''


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* '''Aug-06 Th'''
* '''Sep-04 Fr'''
:{|
Ā 
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br>
- Ā»'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''Ā«
|}
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* '''Aug-07 Fr'''
== Jul 2020 ==


2020 Week 31
* '''Sep-05 Sa''' Ā 
* '''Jul-27 Mo''' Ā 
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:{|
* '''Sep-06 So''' Ā 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
::::* Abstract submission deadline - Ā»[[ESSR 2020 Innsbruck AT]]Ā«
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''
Ā  2020 Week 37
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br>
- Ā»[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]Ā«
|} Ā 


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* '''Sep-07 Mo''' Ā 
* '''Jul-28 Tu''' - World Hepatitis Day
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
| '''Why it is necessary to perform multiple H<sub>2</sub>O<sub>2</sub> calibrations when using the [[Amplex UltraRed]] (AmR) assay?'''<br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H<sub>2</sub>O<sub>2</sub> calibration before sample addition, after sample addition and multiple times during the experiment.Ā  <br>
- Ā»[[Komlodi 2018 Methods Mol Biol]]Ā« <br>


| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H<sub>2</sub>O<sub>2</sub> calibrations implemented. <br>
|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br>
- Ā» [https://www.bioblast.at/index.php/SUITbrowser SUITbrowser]Ā« <br>
: from the O2k-Network Ā»[[IT Foggia Capitanio N]]Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā«
*Ā  Ā  Ā  Ā  Ā  Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1Ī±-mediated glycolytic adaptation. J Virol 84:647-60.Ā  [[Ripoli 2009 J Virol|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Jul-29 We''' Ā 
* '''Sep-08 Tu'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung'''<br>
:from the O2k-Network Ā»[[DE Giessen Weissmann N]]Ā«, Ā»[[ FI Tampere Dufour E]]Ā«, Ā»[[FI Helsinki Jacobs HT]]Ā« and Ā»[[DE Jena Szibor M]]Ā«
*Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. [[Sommer 2020 Sci Adv|Ā»Bioblast linkĀ«]]
|}
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* '''Sep-09 We'''


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* '''Jul-30 Th'''
* '''Sep-10 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br>
|''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br>
- Ā»'''[[Chlororespiration]]'''Ā«
OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P<sub>i</sub>, fuel substrates and O<sub>2</sub>. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of [[excess E-P capacity]] due to kinetic limitations of the phosphorylation system. <br>
- Ā»'''[[BEC_2020.1_doi10.26124bec2020-0001.v1|BEC: Mitochondrial physiology]]'''Ā« - Table 3 and several figures. <br>
- communicated by Ā» [[Cecatto Cristiane]] Ā« and Ā» [[Komlodi Timea]] Ā«
|}
|}
----
----
* '''Jul-31 Fr'''
* '''Sep-11 Fr'''


Ā  2020 Week 30
Ā  2020 Week 38
Ā 
* '''Sep-14 Mo''' Ā 
* '''Jul-20 Mo''' Ā 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="100" align="center" | [[Image:MitoGlobal.jpg|45px|link=https://www.mitoglobal.org/index.php/MitoGlobal_Events|MitoGlobal Events]]
|'''How can you compare respirometric experiments using an internal normalization?'''<br>
| '''World Mitochondrial Disease Week''' ā€“ Sep 13-19
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the ā€œO2 analysis template DL7.4ā€.
World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.
<br>
- Ā»[[Flux control ratio]]Ā«


Read more: Gnaiger Erich ''et al'' ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Ā«
See what is happening near you - Ā»[https://mitochondrialdiseaseweek.org/events/ https://mitochondrialdiseaseweek.org/events/]
|}
|}
----
----
* '''Jul-21 Tu'''
* '''Sep-15 Tu''' - World Lymphoma Awareness Day
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br>
|'''OXPHOS as a potential therapeutic target in classical Hodgkin lymphoma'''<br>
:from the O2k-Network Ā»[[US TN Nashville Wasserman DH]]Ā« and Ā»[[US MN Minneapolis Hughey C]]Ā« Ā 
:from the O2k-Network Ā»[[DE Frankfurt Droese S]]Ā«, Ā»[[DE Frankfurt Wittig I]]Ā« and Ā»[[NL Nijmegen Brandt U]]Ā«
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|Ā»Bioblast linkĀ«]]
*Birkenmeier K, Droese S, Wittig I, Winkelmann R, KƤfer V, Doering C, Hartmann S, Wenz T, Reichert AS, Brandt U, Hansmann ML (2016) Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma are highly dependent on oxidative phosphorylation. Int J Cancer 138:2231-46. [[Birkenmeier 2016 Int J Cancer|Ā»Bioblast linkĀ«]]
|}
|}
----
----
* '''Jul-22 We''' Ā 
* '''Sep-16 We'''
:{|


Ā 
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
----
|''' Why are permeabilized muscle fibers (pfi) a poor sample type for studying reactive oxygen species (ROS) production?Ā  '''<br>
* '''Jul-23 Th'''
In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation. However, the high oxygen pressures used may artificially increase ROS, including H<sub>2</sub>O<sub>2</sub> production, making pfi a less optimal model for ROS production studies. <br>
:{|
- Ā»'''[[Permeabilized_muscle_fibers#Oxygen_dependence_of_ROS_production_-_are_permeabilized_fibers_a_valid_model.3F|Oxygen dependence of ROS production in pfi]]'''Ā« <br>
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā«
| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. TĆ­mea KomlĆ³di]] ''' Ā 
|}
|}
----
----
* '''Jul-24 Fr''' Ā 
* '''Sep-17 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' Ā 
|''' Did you know that you can measure the respiratory activity available for phosphorylation of ADP to ATP in living cells?'''<br>
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br>
SUIT protocols from the [[SUIT-003]] family will guide you through the titrations needed to analyze [[free ROUTINE activity]].<br>
- Ā»'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''Ā«
- Ā»'''[[SUIT-003 O2 ce D009]]'''Ā« and '''Ā»[[SUIT-003 O2 ce-pce D020]]'''Ā« <br>
- communicated by Ā»[[Cardoso Luiza]]Ā« and Ā»[[Komlodi Timea]]Ā«
|}
|}


----
* '''Sep-18 Fr'''


2020 Week 29
----
* '''Sep-19 Sa'''
----
* '''Sep-20 So'''
:::: [[File:ESCI.JPG|100px|link=https://wiki.oroboros.at/index.php/ESCI_2020_Virtual_Event]] <big>'''[[ESCI 2020 Virtual Event]]'''</big> Sep 20-30


* '''Jul-13 Mo''' Ā 
----
Ā 
2020 Week 39
Ā 
* '''Sep-21 Mo''' - [[ESCI 2020 Virtual Event]]
:::: [[File:Oegmbt.png|100px|link=https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Graz_AT|ƖGMBT Graz AT]] <big>'''[[12th ƖGMBT Annual Meeting 2020 Virtual Event]]'''</big> Sep 21-23
Ā 
- World Alzheimer's Day
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br>
|'''Olive polyphenols prove promising in the protection against mitochondrial dysfunction due to Alzheimer's disease and brain aging'''<br>
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ā€˜Copy from fileā€™ option in the ā€˜Slope configurationā€™ window.
:from the O2k-Network Ā»[[DE Giessen Eckert GP]]Ā«
For more information, see:
*Grewal R, Reutzel M, Dilberger B, Hein H, Zotzel J, Marx S, Tretzel J, Sarafeddinov A, Fuchs C, Eckert GP (2020) Purified oleocanthal and ligstroside protect against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing. Exp Neurol 328:113248. [[Grewal 2020 Exp Neurol|Ā»Bioblast linkĀ«]]
- Ā»[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]Ā«
|}
|}
----
* '''Jul-14 Tu'''


:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br>


:from the O2k-Network Ā»[[BR Ribeirao Preto Alberici LC]]Ā« Ā 
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|Ā»Bioblast linkĀ«]]
|'''Resistance training effects in Alzheimerā€™s disease mouse model''' <br>
: from the O2k-Network Ā»[[US FL Boca Raton Khamoui AV]]Ā« <br>
* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Dis 2020:Article ID 5919501. [[Pena 2020 Int J Chronic Dis|Ā»Bioblast linkĀ«]]
|}
|}
----
----
* '''Jul-15 We''' Ā 
* '''Sep-22 Tu''' - [[12th ƖGMBT Annual Meeting 2020 Virtual Event]] , [[ESCI 2020 Virtual Event]]
Ā 
::::
Ā 
----
* '''Jul-16 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
|''' Did you know the reverse electron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using [[Amplex UltraRed]] assay?'''<br>
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br>
The reverse electron flow, also called reverse electron transfer (RET), happens when electrons are transferred from Complex II to the Q-junction and then back to Complex I at high mt-membrane potential. This phenomenon results in high hydrogen peroxide production in some mitochondrial preparations. The SUIT-026 protocol was designed to investigate this effect. <br>
- Ā»'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''Ā« and Ā»'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''Ā«
- Ā»'''[[SUIT-026]]'''Ā« <br>
- communicated by Ā»[[Komlodi Timea]]Ā« and Ā»[[Cardoso Luiza]]Ā« Ā 
|}
|}
----
----
* '''Jul-17 Fr'''
* '''Sep-23 We''' - [[12th ƖGMBT Annual Meeting 2020 Virtual Event]] , [[ESCI 2020 Virtual Event]]
Ā 
----
----
* '''Sep-24 Th''' - [[ESCI 2020 Virtual Event]]
::::* 18:00 - Houska Awards 2020 Vienna - [https://bcgruppe.at/houskapreis/ Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien]


----
* '''Sep-25 Fr''' -[[ESCI 2020 Virtual Event]]
2020 Week 40


2020 Week 28
* '''Sep-28 Mo''' - [[ESCI 2020 Virtual Event]]


* '''Jul-06 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br>
|'''Are whitish deposits forming on the glass wall of the O2k-Chamber? '''<br>
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear.
These deposits might be an accumulation of proteins and subsequent embedding of other biological material or small glass splinters, and a sign that the O2k-Chamber requires cleaning. <br>
For more information, see:<br>
For further details on how to clean the O2k-Chamber properly, see: <br>
- Ā»[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]Ā« and Ā»[[DatLab error messages]] Ā«
- Ā»[[O2k-chamber#Cleaning_the_O2k-chamber|Cleaning the O2k-chamber]]Ā«<br>
- communicated by Ā»[[Komlodi Timea]]Ā«, Ā»Sabine SchmittĀ«, and Ā»Lisa Tindle-SolomonĀ«
|}
|}
----
----
* '''Jul-07 Tu''' Ā 
* '''Sep-29 Tu''' - [[ESCI 2020 Virtual Event]]
Ā 
: World Heart Day
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br>
|'''Succinate dehydrogenase inhibition protects myocardium from ischemia reperfusion injury but efficacy may be influenced by diabetes'''<br>
:from the O2k-Network Ā»[[FR Lille Duez H]]Ā« Ā 
:from the O2k-Network Ā»[[DK Aarhus Boetker HE]]Ā« and Ā»[[DK Copenhagen Larsen S]]Ā«
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|Ā»Bioblast linkĀ«]]
*Jespersen NR, Hjortbak MV, Lassen TR, StĆøttrup NB, Johnsen J, Tonnesen PT, Larsen S, Kimose HH, BĆøtker HE (2020) Cardioprotective effect of succinate dehydrogenase inhibition in rat hearts and human myocardium with and without diabetes mellitus. Sci Rep 10:10344. [[Jespersen 2020 Sci Rep|Ā»Bioblast linkĀ«]]
|}
:{|
| width="100" align="center" |[[Image:EU-logo.jpg|70px|link=https://wiki.oroboros.at/index.php/Projects |Projects]]
|As a Horizon 2020 beneficiary, Oroboros signed the '''[https://ec.europa.eu/info/research-and-innovation/research-area/health-research-and-innovation/coronavirus-research-and-innovation/covid-research-manifesto_en Manifesto for EU COVID-19 Research]''', showing our support for the accessibility of research results in the fight against COVID-19.<br>
Ā 
|}
|}
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* '''Jul-08 We'''


* '''Sep-30 We''' - [[ESCI 2020 Virtual Event]]
::::* Early registration deadline - Ā»[[ESSR 2020 Innsbruck AT]]Ā«
----
----
* '''Jul-09 Th'''


:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br>
- Ā»'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''Ā«
|}
----
* '''Jul-10 Fr'''


== Jun 2020 ==
</div>
Ā  2020 Week 27
</div>


* '''Jun-29 Mo'''
== Aug 2020 ==
Ā 
[[File:Expand.png|right|35px |Click to expand or collaps]]
Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā 
2020 Week 32
* '''Aug-03 Mo''' Ā 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] Ā 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br>
:An 'O2 slope neg.' within Ā± 2 pmolāˆ™s<sup>āˆ’1</sup>āˆ™mL<sup>āˆ’1</sup> is optimal (Ā± 4 pmolāˆ™s<sup>āˆ’1</sup>āˆ™mL<sup>āˆ’1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br>
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br>
- Ā»[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]Ā«
- Ā» [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]Ā«
|}
|}
----
----
* '''Jun-30 Tu'''
* '''Aug-04 Tu'''
Ā 
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br>
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br>
:from the O2k-Network Ā»[[LV Riga Makrecka-Kuka M]]Ā« Ā 
:from the O2k-Network Ā»[[US PA Pittsburgh Jurczak MJ]]Ā« Ā 
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|Ā»Bioblast linkĀ«]]
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|Ā»Bioblast linkĀ«]]
|}
|}
----
----
* '''Jul-01 We''' Ā 
* '''Aug-05 We'''
:{|
Ā 
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br>
:Bring the world of scientific research closer to the world of patients by supporting four cancer patientsā€™ associations.
Ā 
:- Ā»[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html]Ā«
|}
----
----
* '''Jul-02 Th''' Ā 
* '''Aug-06 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br>
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br>
- loss of oxidative capacity during the time course of the respirometric assay,<br>
- Ā»'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''Ā«
- insufficient [[Uncoupler|uncoupler]] concentrations,<br>
|}
- over titration of an uncoupler, which inhibits ET capacity,<br>
----
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.
* '''Aug-07 Fr'''
2020 Week 33


Information from: Gnaiger Erich ''et al'' ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Ā«
----
|}
* '''Aug-10 Mo''' Ā 


----
----
* '''Jul-03 Fr'''
* '''Aug-11 Tu'''
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br>
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br>
: from the O2k-Network Ā»[[US CO Fort Collins Chicco AJ]]Ā« <br>
:from the O2k-Network Ā»[[DE Bremerhaven Mark FC]]Ā«
* Ā  Ā  Ā  Ā  Ā  Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|Ā»Bioblast linkĀ«]]
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |Ā»Bioblast linkĀ«]]
|}
|}


----
----
* '''Aug-12 We'''
:{|
| width="100" align="center" |[[File:MitoFit Preprint Arch pdf.png|100px|link=https://wiki.oroboros.at/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf |MitoFit pdf]]
|'''Some terms in ā€œMitochondrial physiologyā€ ''Bioenergetics Communications'' 2020.1 (States and rates) did not look familiar ā€• what is the elementary unit [x]?'''


2020 Week 26
Fundamental answers, a critical discussion on the SI unit for ''count'', and the meaning of Euclidā€™s unit and Euclidā€™s number are presented in an unusual ā€˜'''canonical review'''ā€™:


* '''Jun-22 Mo'''
* Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 [https://www.mitofit.org/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf doi:10.26124/mitofit:200004]. - [[Gnaiger_MitoFit_Preprint_Arch_2020.4 |'''Ā»Bioblast linkĀ«''']]
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| '''Did you know how a stirrer test can save your experiments?'''<br>
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br>
- Ā»[[Oxygen calibration - DatLab]]Ā«
|}
|}
----
<br />
* '''Jun-23 Tu'''
Ā 
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
Ā 
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br>
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]
:from the O2k-Network Ā»[[IT Verona Calabria E]]Ā«
|Join the discussion on the MitoEAGLE Consortium Communication: ā€œMitochondrial physiologyā€ ''Bioenergetics Communications'' 2020.1
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|Ā»Bioblast linkĀ«]]
Ā 
|}
Ā» [https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1#On_a_Comment_in_Nature_Metabolism '''On a Comment in ''Nature Metabolism'''''] Ā«
----
|}
* '''Jun-24 We'''


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* '''Jun-25 Th'''
* '''Aug-13 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br>
| Photosynthesis efficiency is reduced by 25 % in plants and algae due the metabolic process called photorespiration.Ā  <br>
- Ā» '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''Ā«
- Ā»'''[[Photorespiration]]'''Ā«
|}
|}
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* '''Jun-26 Fr'''
* '''Aug-14 Fr'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Ā  Ā  Ā  Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br>
: from the O2k-Network Ā»[[NZ Auckland Hickey AJ]]Ā« <br>
*Ā  Ā  Ā  Ā  Ā  Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|Ā»Bioblast linkĀ«]]
|}
2020 Week 34


{|
* '''Aug-17 Mo''' Ā 
Ā  | width="110" align="center" |


[[Image:MitoGlobal.jpg|80px|left|MitoGlobal]]
* '''Aug-18 Tu'''


| Join and disseminate the [https://mitowomen.org/index.html '''ā€œWomen in Mitochondriaā€'''] initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity. <br>
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Mitochondrial respiration in PBMCs from patients with major depression'''<br>
:from the O2k-Network Ā»[[DE Ulm Karabatsiakis A]]Ā« and Ā»[[DE Ulm Radermacher P]]Ā«
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |Ā»Bioblast linkĀ«]]
|}
|}


2020 Week 25
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* '''Aug-19 We'''


* '''Jun-15 Mo'''
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* '''Aug-20 Th'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br>
- Ā» [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefactĀ  Injection artefact]Ā«
<br>
- Ā»'''[[Q-Module]]'''Ā« Ā 
|}
|}
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* '''Jun-16 Tu''' Ā 
* '''Aug-21 Fr'''
2020 Week 35
Ā 
* '''Aug-24 Mo''' Ā 
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br>
|'''Can NADH be used as an externally added substrate to obtain the [[NADH electron transfer-pathway state]] in [[Mitochondrial_respiration |mitochondrial respiration]]? '''<br>
:from the O2k-Network Ā»[[CA Montreal Hepple RT]]Ā« Ā 
No, in most types of intact mitochondria NADH cannot pass the [[mitochondrial inner membrane]] (mtIM). Yeast mitochondria are an exception. <br>
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|Ā»Bioblast linkĀ«]]
- Ā»[[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide]]Ā« and Ā»[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]Ā«
|}
|}
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* '''Jun-17 We'''
* '''Aug-25 Tu'''
Ā 
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* '''Jun-18 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''
|'''Low-intensity exercise increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults'''<br>
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br>
:from the O2k-Network Ā»[[LV Riga Makrecka-Kuka M]]Ā«
- Ā»'''[[SUIT-003 O2 ce D037]]'''Ā« and Ā»'''[[SUIT-003 O2 ce D038]]'''Ā«
*Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. ā€” [[Liepinsh 2020 Physiol Rep|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Jun-19 Fr'''
* '''Aug-26 We''' Ā 


Ā  2020 Week 24
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| '''Non-mitochondrial respiration or [[residual oxygen consumption]] (''Rox'')? ā€” Concept-driven constructive terminology frames our perception.'''
After inhibition of electron-transfer (ET) pathways or in the absence of fuel substrates, both ''mitochondrial'' non-ET reactions and ''non-mitochondrial'' reactions contribute to ''residual oxygen consumption'' in mt-preparations and living cells. <br>
- Ā»'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''Ā« ā€” Table 1: ''Rox'' induced by non-ET pathway oxidation reactions
|}


* '''Jun-08 Mo'''
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:{|
* '''Aug-27 Th''' Ā 
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Celebrating World Oceans Day'''<br>


Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br>
- Ā»'''[[PhotoBiology]]'''Ā«
|}
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* '''Jun-09 Tu''' Ā 
* '''Aug-28 Fr'''


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* '''Jun-10 We'''
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* '''Jun-11 Th'''
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br>
- Ā»'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''Ā«
|}


</div>
</div>


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== Jul 2020 ==
* '''Jun-15 Th'''
[[File:Expand.png|right|35px |Click to expand or collaps]]
Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā  2020 Week 27
* '''Jul-01 We''' Ā 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]
|''' Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br>
- Ā» [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefactĀ  Injection artefact]Ā«
:Bring the world of scientific research closer to the world of patients by supporting four cancer patientsā€™ associations.
Ā 
:- Ā»[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html]Ā«
|}
|}
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* '''Jun-12 Fr'''
* '''Jul-02 Th''' Ā 
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ<sub>10</sub> in the platelets of CKD patients''' <br>
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br>
: from the O2k-Network Ā» [[ SK Bratislava Sumbalova Z]] Ā« <br>
- loss of oxidative capacity during the time course of the respirometric assay,<br>
* GvozdjĆ”kovĆ” A, SumbalovĆ” Z, KucharskĆ” J, KomlĆ³si M, RausovĆ” Z, VančovĆ” O, SzĆ”moÅ”ovĆ” M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q<sub>10</sub> and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|Ā»Bioblast linkĀ«]]
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> Ā 
- over titration of an uncoupler, which inhibits ET capacity,<br>
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.


Information from: Gnaiger Erich ''et al'' ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Ā«
|}
|}


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* '''Jul-03 Fr'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br>
: from the O2k-Network Ā»[[US CO Fort Collins Chicco AJ]]Ā« <br>
*Ā  Ā  Ā  Ā  Ā  Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|Ā»Bioblast linkĀ«]]
|}


Ā  2020 Week 23
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Ā  2020 Week 28


* '''Jun-01 Mo''' Ā 
* '''Jul-06 Mo''' Ā 
:{|
:{|
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br>
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br>
- Ā»[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_testĀ  Unstable 'O2 slope neg.']Ā« and Ā»[[TIP2k Filter Papers]]Ā«
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear.
For more information, see:<br>
- Ā»[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]Ā« and Ā»[[DatLab error messages]] Ā«
|}
|}
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* '''Jun-02 Tu''' Ā 
* '''Jul-07 Tu''' Ā 
Ā 
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br>
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br>
:from the O2k-Network Ā»[[AT Innsbruck Gnaiger E]]Ā« , Ā»[[CA Rimouski Blier PU]]Ā« and Ā»[[CA Edmonton Lemieux H]]Ā« Ā 
:from the O2k-Network Ā»[[FR Lille Duez H]]Ā« Ā 
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|Ā»Bioblast linkĀ«]]
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Jun-03 We''' Ā 
* '''Jul-08 We''' Ā 


----
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* '''Jun-04 Th'''
* '''Jul-09 Th'''
Ā 
:{|
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Mitochondria show variable preferences for [[GM|glutamate & malate]] or [[PM|pyruvate & malate]] to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.''' <br>
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br>
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates. <br>
- Ā»'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''Ā«
-Ā»'''[[SUIT-008]]'''Ā« and Ā»'''[[SUIT-014]]'''Ā«
|}
|}
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* '''Jun-05 Fr'''
* '''Jul-10 Fr'''
Ā 
Ā  2020 Week 29
Ā 
* '''Jul-13 Mo''' Ā 
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== May 2020 ==
2020 Week 22
Ā 
* '''May-25 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br>
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br>
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br>
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ā€˜Copy from fileā€™ option in the ā€˜Slope configurationā€™ window.
- Ā»[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]Ā«
For more information, see:
- Ā»[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]Ā«
|}
|}
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* '''May-26 Tu''' Ā 
* '''Jul-14 Tu'''
Ā 
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br>
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br>
: from the O2k-Network Ā» [[ AT Innsbruck Gnaiger E]] Ā« and Ā» [[ AT Innsbruck Oroboros]] Ā« <br>
* Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Santā€™Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487.Ā  [[Schoepf 2020 Nat Commun|Ā»Bioblast linkĀ«]]


:from the O2k-Network Ā»[[BR Ribeirao Preto Alberici LC]]Ā«
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|Ā»Bioblast linkĀ«]]
|}
|}
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* '''May-27 We''' Ā 
* '''Jul-15 We''' Ā 
:{|
| width="100" align="center" |[[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
|The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine


To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus


|}
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* '''May-28 Th''' Ā 
* '''Jul-16 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br>
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
- Ā»'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injectionĀ  Nitrogen injection]'''Ā«
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br>
- Ā»'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''Ā« and Ā»'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''Ā«
|}
|}
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* '''May-29 Fr'''
* '''Jul-17 Fr'''
----
Ā 
2020 Week 30


* '''Jul-20 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can you compare respirometric experiments using an internal normalization?'''<br>
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the ā€œO2 analysis template DL7.4ā€.
<br>
- Ā»[[Flux control ratio]]Ā«


Read more: Gnaiger Erich ''et al'' ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Ā«
|}
----
* '''Jul-21 Tu'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br>
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br>
:from the O2k-Network Ā»[[BR Sao Paulo Silber AM]]Ā« Ā 
:from the O2k-Network Ā»[[US TN Nashville Wasserman DH]]Ā« and Ā»[[US MN Minneapolis Hughey C]]Ā« Ā 
*Alberto LuĆ©vano-MartĆ­nez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print].Ā  [[Alberto Luevano-Martinez 2020 FEBS Lett|Ā»Bioblast linkĀ«]]
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print].Ā  [[Lantier 2020 Diabetes|Ā»Bioblast linkĀ«]]
|}
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2020 Week 21
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* '''Jul-22 We'''
Ā 


* '''May-18 Mo''' Ā 
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* '''Jul-23 Th''' Ā 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] Ā 
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]
|'''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br>
| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. TĆ­mea KomlĆ³di]] '''
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br>
- Ā»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]Ā«
|}
|}
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* '''May-19 Tu''' Ā 
* '''Jul-24 Fr''' Ā 
:{|
:{|
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''Ā«<br>
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' Ā 
:from the O2k-Network Ā»[[Charles Anne-Laure]]Ā« Ā»[[Geny Bernard]]Ā«
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br>
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|Ā»Bioblast linkĀ«]]
- Ā»'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''Ā«
|}
|}
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Ā 
* '''May-20 We''' Ā 
Ā 
2020 Week 31
* '''Jul-27 Mo''' Ā 
:{|
:{|
| width="100" align="center"|[[Image:SI-units.png|65px|link=https://wiki.oroboros.at/index.php/BEC2020.1_doi10.26124bec2020-0001.v1#SI_units|SI Units]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''SI units'''<br>
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''
One year ago, on [https://www.euramet.org/publications-media-centre/news/?tx_news_pi1%5Bnews%5D=787&tx_news_pi1%5Baction%5D=detail&tx_news_pi1%5Bcontroller%5D=News World Metrology Day] (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of '''Mitochondrial physiology''' and with the launch of an innovative journal ā€” '''[[Bioenergetics Communications]]'''.
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br>
|}
- Ā»[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]Ā«
|}


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* '''Jul-28 Tu''' - World Hepatitis Day
:{|
:{|
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]
|MitoEAGLE ''Consortium Communication'': '''Mitochondrial physiology''' ā€• Published online: 2020-05-20.
Gnaiger E et al ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1<br>
Ā» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1
|}


| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br>
: from the O2k-Network Ā»[[IT Foggia Capitanio N]]Ā« <br>
*Ā  Ā  Ā  Ā  Ā  Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1Ī±-mediated glycolytic adaptation. J Virol 84:647-60.Ā  [[Ripoli 2009 J Virol|Ā»Bioblast linkĀ«]]
|}
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* '''Jul-29 We'''


* '''May-21 Th'''
----
Ā 
* '''Jul-30 Th'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br>
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br>
-Ā»'''[[PhotoBiology]]'''Ā«
- Ā»'''[[Chlororespiration]]'''Ā«
|}
|}
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* '''May-22 Fr'''
* '''Jul-31 Fr'''
Ā 
Ā 
Ā 
</div>
</div>
== Jun 2020 ==
Ā 
[[File:Expand.png|right|35px |Click to expand or collaps]]


Ā  2020 Week 20
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">


* '''May-11 Mo'''
2020 Week 23


* '''Jun-01 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
|'''Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.'''<br>
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br>
Learn more about steady states including performance and mark setting for data analysis <br>
- Ā»[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_testĀ  Unstable 'O2 slope neg.']Ā« and Ā»[[TIP2k Filter Papers]]Ā«
- Ā»[[Steady state]]Ā«
|}
|}
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* '''May-12 Tu''' Ā 
* '''Jun-02 Tu''' Ā 
:{|
:{|
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''Mitochondria - a driving force in adipocyte differentiation''' <br>
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br>
:from the O2k-Network Ā»[[CZ Pilsen Kuncova J]]Ā« <br>
:from the O2k-Network Ā»[[AT Innsbruck Gnaiger E]]Ā« , Ā»[[CA Rimouski Blier PU]]Ā« and Ā»[[CA Edmonton Lemieux H]]Ā« Ā 
* KladnickĆ” I, ČedĆ­kovĆ” M, KripnerovĆ” M, DvoÅ™Ć”kovĆ” J, KohoutovĆ” M, TÅÆma Z, MĆ¼llerovĆ” D, KuncovĆ” J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|Ā»Bioblast linkĀ«]]
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|Ā»Bioblast linkĀ«]]
|}
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* '''May-13 We'''
* '''Jun-03 We''' Ā 


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* '''May-14 Th'''
* '''Jun-04 Th'''
Ā 
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
|How can I measure '''ATP production''' with the O2k?Ā  <br>
| '''Mitochondria show variable preferences for [[GM|glutamate & malate]] or [[PM|pyruvate & malate]] to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.''' <br>
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br>
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates. <br>
- Ā»'''[[Magnesium Green]]'''Ā«
-Ā»'''[[SUIT-008]]'''Ā« and Ā»'''[[SUIT-014]]'''Ā«
|}
|}
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* '''May-15 Fr'''
* '''Jun-05 Fr'''
Ā 
Ā 
2020 Week 24
Ā 
* '''Jun-08 Mo''' Ā 
:{|
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br>
| '''Celebrating World Oceans Day'''<br>
: from the O2k-Network Ā»[[ FI Helsinki Jacobs HT]]Ā« , Ā»[[ DE Magdeburg Gellerich FN]]Ā« , Ā»[[FI Tampere Dufour E]]Ā« , Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā« and Ā»[[DE Frankfurt Wittig I]]Ā«<br>
Ā 
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|Ā»Bioblast linkĀ«]]
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br>
- Ā»'''[[PhotoBiology]]'''Ā«
|}
|}
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* '''May-17 Su''' World Hypertension Day
* '''Jun-09 Tu'''
Ā 
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* '''Jun-10 We'''
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* '''Jun-11 Th'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br>
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br>
:from the O2k-Network Ā»[[CZ Prague Houstek J]]Ā« and Ā»[[CZ Prague Kalous M]]Ā«
- Ā»'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''Ā«
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|Ā»Bioblast linkĀ«]]
|}
|}
2020 Week 19


* '''May-04 Mo'''
Ā 
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* '''Jun-15 Th'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br>
|''' Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''
Perform and apply the instrumental background test:<br>
- Ā» [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefactĀ  Injection artefact]Ā«
- Ā»[[Flux / Slope| O2 background correction in DatLab]]Ā« and Ā»[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]Ā«
|}
|}
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* '''May-05 Tu'''
* '''Jun-12 Fr'''
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''
::::::: - from the O2k-Network Ā»[[FI Helsinki Jacobs HT]]Ā«Ā  Ā»[[DE Magdeburg Gellerich FN]]Ā« Ā»[[FI Tampere Dufour E]]Ā« Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā« Ā»[[DE Frankfurt Wittig I]]Ā« and Ā»[[UK Brighton Moore AL]]Ā«
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|Ā»Bioblast linkĀ«]]
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* '''May-06 We'''
Ā 
----
* '''May-07 Th'''
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Ā 
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: Ā»'''[[Malate-anaplerotic pathway control state]]'''Ā«
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ<sub>10</sub> in the platelets of CKD patients''' <br>
: from the O2k-Network Ā» [[ SK Bratislava Sumbalova Z]] Ā« <br>
* GvozdjĆ”kovĆ” A, SumbalovĆ” Z, KucharskĆ” J, KomlĆ³si M, RausovĆ” Z, VančovĆ” O, SzĆ”moÅ”ovĆ” M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q<sub>10</sub> and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|Ā»Bioblast linkĀ«]]


- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - Ā»'''[[SUIT-027 |SUIT-027]]'''Ā«
|}
Ā  2020 Week 25
* '''Jun-15 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''
- Ā» [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefactĀ  Injection artefact]Ā«
|}


- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - Ā»'''[[SUIT-002 |SUIT-002]]'''Ā«
----
* '''Jun-16 Tu'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br>
:from the O2k-Network Ā»[[CA Montreal Hepple RT]]Ā«
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|Ā»Bioblast linkĀ«]]
|}
|}
<br />
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* '''Jun-17 We'''


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* '''May-08 Fr'''
* '''Jun-18 Th'''
Ā 
:{|
== Apr 2020 ==
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
Ā 
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''
2020 Week 18
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br>
Ā 
- Ā»'''[[SUIT-003 O2 ce D037]]'''Ā« and Ā»'''[[SUIT-003 O2 ce D038]]'''Ā«
* '''Apr-27 Mo'''
|}
:::: [[Image:O2k-Support.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''
::::::: The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub>Ā  concentrations. - Ā»[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]Ā«
Ā 


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----
* '''Apr-28 Tu''' Ā 
* '''Jun-19 Fr'''
::::[[Image:O2k-Publications.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''
::::::: - from the O2k-Network Ā»[[FI Helsinki Jacobs HT]]Ā«, Ā»[[DE Magdeburg Gellerich FN]]Ā« and Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā«
::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, LeƟmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - [[Szibor 2020 J Biol Chem|Ā»Bioblast linkĀ«]]


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2020 Week 26
Ā 
* '''Apr-29 We'''


* '''Jun-22 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| '''Did you know how a stirrer test can save your experiments?'''<br>
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br>
- Ā»[[Oxygen calibration - DatLab]]Ā«
|}
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* '''Apr-30 Th'''
* '''Jun-23 Tu'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br>
:from the O2k-Network Ā»[[IT Verona Calabria E]]Ā«
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|Ā»Bioblast linkĀ«]]
|}
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* '''Jun-24 We'''


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* '''May-01 Fr'''
* '''Jun-25 Th'''
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br>
- Ā» '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''Ā«
|}


2020 Week 17
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* '''Apr-20 Mo''' Ā 
* '''Jun-26 Fr'''


:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''
{|
| width="110" align="center" | Ā 


::::::: - from the O2k-Network Ā»[[US TN Nashville Wasserman DH]]Ā« and Ā»[[US MI East Lansing Bazil JN]]Ā«
[[Image:MitoGlobal.jpg|80px|left|MitoGlobal]]
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523.Ā  - [[Wollenman 2017 PLOS ONE |Ā»Bioblast linkĀ«]]


| Join and disseminate the [https://mitowomen.org/index.html '''ā€œWomen in Mitochondriaā€'''] initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity. <br>
|}


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
Ā  2020 Week 27
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Ā»[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]Ā«
* '''Jun-29 Mo'''
Ā 
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] Ā 
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
:An 'O2 slope neg.' within Ā± 2 pmolāˆ™s<sup>āˆ’1</sup>āˆ™mL<sup>āˆ’1</sup> is optimal (Ā± 4 pmolāˆ™s<sup>āˆ’1</sup>āˆ™mL<sup>āˆ’1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br>
- Ā»[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]Ā«
|}
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Ā 
* '''Jun-30 Tu'''
* '''Apr-21 Tu''' Ā 
[[File:CS.png|right|100px|Citrate synthase]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br>
- Ā»'''[[MiPNet17.04 CitrateSynthase]]'''Ā«
:from the O2k-Network Ā»[[LV Riga Makrecka-Kuka M]]Ā«
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|Ā»Bioblast linkĀ«]]
|}
|}
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* '''Apr-22 We'''


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* '''Apr-23 Th'''


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</div>
* '''Apr-24 Fr''' Ā 
</div>
[[File:ETC-.JPG|right|150px|Electron transport chain]]
Ā 
== May 2020 ==
Ā 
[[File:Expand.png|right|35px |Click to expand or collaps]]
Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā 
2020 Week 19
Ā 
* '''May-04 Mo'''
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]].
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br>
- Ā»'''[[Hatefi 1962 J Biol Chem-XLII]]'''Ā«
Perform and apply the instrumental background test:<br>
- Ā»[[Flux / Slope| O2 background correction in DatLab]]Ā« and Ā»[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]Ā«
|}
|}
<br />


Ā  2020 Week 16
* '''Apr-13 Mo'''
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - Ā»'''[[SUIT-024 |SUIT-024]]'''Ā«
|}
<br />
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* '''Apr-14 Tu''' Ā 
* '''May-05 Tu''' Ā 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - Ā»[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]Ā«
::::::: - from the O2k-Network Ā»[[FI Helsinki Jacobs HT]]Ā«Ā  Ā»[[DE Magdeburg Gellerich FN]]Ā« Ā»[[FI Tampere Dufour E]]Ā« Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā« Ā»[[DE Frankfurt Wittig I]]Ā« and Ā»[[UK Brighton Moore AL]]Ā«
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|Ā»Bioblast linkĀ«]]
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* '''May-06 We'''


* '''Apr-15 We'''
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* '''May-07 Th'''
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: Ā»'''[[Malate-anaplerotic pathway control state]]'''Ā«


* '''Apr-16 Th'''
- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - Ā»'''[[SUIT-027 |SUIT-027]]'''Ā«
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''


::::::: - from the O2k-Network Ā»[[DE Frankfurt Eckert GP]]Ā« Ā»[[CH Basel Eckert A]]Ā«
- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - Ā»'''[[SUIT-002 |SUIT-002]]'''Ā«
::::::* Leuner K, Schulz K, SchĆ¼tt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, MĆ¼ller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204.Ā  - [[Leuner 2012 Mol Neurobiol|Ā»Bioblast linkĀ«]]
|}
<br />
<br />
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* '''Apr-17 Fr'''
* '''May-08 Fr'''
Ā  2020 Week 15
2020 Week 20
* '''May-11 Mo'''


* '''Apr-06 Mo'''
:{|
Ā 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
<big><big>{{Template:Humankind COVID-19}}</big></big>
|'''Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.'''<br>
Ā 
Learn more about steady states including performance and mark setting for data analysis <br>
Ā 
- Ā»[[Steady state]]Ā«
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout''' Ā 
|}
::::::: - from the O2k-Network Ā»[[CH Basel Eckert A]]Ā«
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|Ā»Bioblast linkĀ«]]


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* '''May-12 Tu'''
:{|
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''Mitochondria - a driving force in adipocyte differentiation''' <br>
:from the O2k-Network Ā»[[CZ Pilsen Kuncova J]]Ā« <br>
* KladnickĆ” I, ČedĆ­kovĆ” M, KripnerovĆ” M, DvoÅ™Ć”kovĆ” J, KohoutovĆ” M, TÅÆma Z, MĆ¼llerovĆ” D, KuncovĆ” J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|Ā»Bioblast linkĀ«]]
|}
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* '''May-13 We'''


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* '''Apr-07 Tu''' Ā 
* '''May-14 Th'''
:::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''''
Ā 
::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - Ā»[[SUIT: Browse DL-Protocols and templates]]Ā«


:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]
|How can I measure '''ATP production''' with the O2k?Ā  <br>
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br>
- Ā»'''[[Magnesium Green]]'''Ā«
|}
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* '''Apr-08 We''' Ā 
* '''May-15 Fr'''
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''
:{|
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - Ā»'''[[SUIT-010 |SUIT-010]]'''Ā«
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
Ā 
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br>
: from the O2k-Network Ā»[[ FI Helsinki Jacobs HT]]Ā« , Ā»[[ DE Magdeburg Gellerich FN]]Ā« , Ā»[[FI Tampere Dufour E]]Ā« , Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā« and Ā»[[DE Frankfurt Wittig I]]Ā«<br>
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|Ā»Bioblast linkĀ«]]
|}
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* '''Apr-09 Th'''
* '''May-17 Su''' World Hypertension Day
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br>
:from the O2k-Network Ā»[[CZ Prague Houstek J]]Ā« and Ā»[[CZ Prague Kalous M]]Ā«
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|Ā»Bioblast linkĀ«]]
|}


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Ā  2020 Week 21
* '''Apr-10 Fr'''
Ā 
Ā  2020 Week 14
Ā 
* '''Mar-30 Mo'''
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā  [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' Ā»'''[[Enable DL-Protocol editing]]'''Ā«


* '''May-18 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
|'''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br>
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br>
- Ā»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]Ā«
|}
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* '''Mar-31 Tu''' Ā 
* '''May-19 Tu''' Ā 
Ā 
:{|
Ā 
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''Ā«<br>
:from the O2k-Network Ā»[[Charles Anne-Laure]]Ā« Ā»[[Geny Bernard]]Ā«
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|Ā»Bioblast linkĀ«]]
|}
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* '''Apr-01 We''' Ā 
* '''May-20 We''' Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinsonā€™s Disease Awareness Month:''' Dominant mutation in Parkinsonā€™s Disease causes loss of protein function in mitochondria''' Ā 
:{|
::::::: - from the O2k-Network Ā»[[FR Angers Gueguen N]]Ā« and Ā»[[BE Leuven Spinazzi M]]Ā«
| width="100" align="center"|[[Image:SI-units.png|65px|link=https://wiki.oroboros.at/index.php/BEC2020.1_doi10.26124bec2020-0001.v1#SI_units|SI Units]]
::::::* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|Ā»Bioblast linkĀ«]]
|'''SI units'''<br>
One year ago, on [https://www.euramet.org/publications-media-centre/news/?tx_news_pi1%5Bnews%5D=787&tx_news_pi1%5Baction%5D=detail&tx_news_pi1%5Bcontroller%5D=News World Metrology Day] (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of '''Mitochondrial physiology''' and with the launch of an innovative journal ā€” '''[[Bioenergetics Communications]]'''.
|}
Ā 
:{|
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]
|MitoEAGLE ''Consortium Communication'': '''Mitochondrial physiology''' ā€• Published online: 2020-05-20.
Gnaiger E et al ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1<br>
Ā» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1
|}


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[[File:Tony hickey garage.jpg|200px|thumb|Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry]]
* '''Apr-02 Th'''
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?'''Ā  Use the SUITbrowser: Ā»'''[[SUITbrowser]]'''Ā«
<br />
<br />


::::[[Image:O2k-Network.png|70px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] Creative solutions coming from the O2k-Network labs - Ā» [[NZ Auckland Hickey AJ]]
* '''May-21 Th'''
<br />
<br />


:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br>
-Ā»'''[[PhotoBiology]]'''Ā«
|}
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* '''Apr-03 Fr'''
* '''May-22 Fr'''
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''
Ā  2020 Week 22


::::::: - from the O2k-Network Ā»[[DE Nuthetal Klaus S]]Ā«
* '''May-25 Mo'''
::::::* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |Ā»Bioblast linkĀ«]] Ā 
:{|
<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
== Mar 2020 ==
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br>
Ā  2020 Week 13
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br>
Ā 
- Ā»[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]Ā«
* '''Mar-23 Mo'''
|}
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* '''May-26 Tu'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Ā  Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br>
: from the O2k-Network Ā» [[ AT Innsbruck Gnaiger E]] Ā« and Ā» [[ AT Innsbruck Oroboros]] Ā« <br>
* Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Santā€™Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. Ā  [[Schoepf 2020 Nat Commun|Ā»Bioblast linkĀ«]]


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''
|}
::::::: '''(1) By adjusting the dithionite concentration toĀ  2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - Ā»'''[[Dithionite|Dithionite]]'''Ā« <br>
----
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - Ā»'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''Ā«
* '''May-27 We'''
:{|
| width="100" align="center" |[[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
|The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine


To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus


|}
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* '''Mar-24 Tu'''
* '''May-28 Th''' Ā 
:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection'''
:{|
::::::: - from the O2k-Network Ā»[[US NC Durham Li PA]]Ā«
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |Ā»Bioblast linkĀ«]]
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br>
- Ā»'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injectionĀ  Nitrogen injection]'''Ā«
|}
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* '''Mar-25 We''' Ā 
* '''May-29 Fr'''
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''
::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? ā€“ An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]


:{|


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: Ā»'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''Ā«
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
----
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br>
* '''Mar-26 Th'''
:from the O2k-Network Ā»[[BR Sao Paulo Silber AM]]Ā« Ā 
*Alberto LuĆ©vano-MartĆ­nez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print].Ā  [[Alberto Luevano-Martinez 2020 FEBS Lett|Ā»Bioblast linkĀ«]]
|}


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* '''Mar-27 Fr'''


Ā  2020 Week 12Ā 


* '''Mar-16 Mo'''
</div>
::::: [[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|60px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]] '''Oroboros operates from now on in home office mode - we stay connected.'''
</div>


== Apr 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]


:::: [[Image:SUIT icon.jpg|80px|left|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''The standardized coupling control protocol to explore the bioenergetics of living cells''' Ā 
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - Ā»'''[[SUIT-003 |SUIT-003]]'''Ā«
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<br />
<div class="mw-collapsible-content">
2020 Week 14
Ā 
* '''Apr-01 We'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinsonā€™s Disease Awareness Month:''' Dominant mutation in Parkinsonā€™s Disease causes loss of protein function in mitochondria''' Ā 
::::::: - from the O2k-Network Ā»[[FR Angers Gueguen N]]Ā« and Ā»[[BE Leuven Spinazzi M]]Ā«
::::::* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|Ā»Bioblast linkĀ«]]


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[[File:Tony hickey garage.jpg|200px|thumb|Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry]]
* '''Apr-02 Th'''
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?'''Ā  Use the SUITbrowser: Ā»'''[[SUITbrowser]]'''Ā«
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<br />


* '''Mar-17 Tu'''
::::[[Image:O2k-Network.png|70px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] Creative solutions coming from the O2k-Network labs - Ā» [[NZ Auckland Hickey AJ]] Ā 
Ā 
<br />
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ''' Zika virus infection leads to mitochondrial dysfunction'''
<br />
::::::: - from the O2k-Network Ā»[[BR Rio de Janeiro Galina A]]Ā«
::::::* Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - [[Ledur 2020 Sci Rep |Ā»Bioblast linkĀ«]]


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* '''Apr-03 Fr'''
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''


* '''Mar-18 We'''
::::::: - from the O2k-Network Ā»[[DE Nuthetal Klaus S]]Ā«
::::::* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print].Ā  - [[Ost 2020 EMBO Rep |Ā»Bioblast linkĀ«]]
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:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Exercise protects against the effects of aging on mitochondrial function'''
Ā  2020 Week 15


::::::: - from the O2k-Network Ā»[[KR Busan Han J]]Ā« Ā»[[KR Incheon Kwak HB]]Ā«
* '''Apr-06 Mo'''
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93.Ā  - [[No 2020 Pflugers Arch |Ā»Bioblast linkĀ«]]
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{{Template:Humankind COVID-19}}
* '''Mar-19 Th'''
:::: [[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis '''Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home'''.
::::::: - Ā» For the download link and password, please email [mailto:instruments@oroboros.at instruments@oroboros.at]
<br />


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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout''' Ā 
* '''Mar-20 Fr'''
::::::: - from the O2k-Network Ā»[[CH Basel Eckert A]]Ā«
::::* '''Abstract submission deadline''' - Ā»[[SHVM 2020 Halifax CA]]Ā«
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|Ā»Bioblast linkĀ«]]


2020 Week 11
* '''Mar-08 Su''' - '''[https://www.internationalwomensday.com/ International Women's Day]'''
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Addressing the underrepresentation of women |Ā»Bioblast linkĀ«]]
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* '''Apr-07 Tu'''
:::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''''


* '''Mar-09 Mo'''
::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - Ā»[[SUIT: Browse DL-Protocols and templates]]Ā«
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] Ā»<big>'''[[MitoEAGLE WG2 Innsbruck AT]]'''</big>Ā« (Mar 09-13)
Ā 
Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Protein-specific mitochondrial respiratory capacity is higher in women than men'''
::::::: - from the O2k-Network Ā»[[SE Stockholm Cardinale DA]]Ā«
::::::* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |Ā»Bioblast linkĀ«]]


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* '''Apr-08 We'''
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - Ā»'''[[SUIT-010 |SUIT-010]]'''Ā«


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* '''Mar-10 Tu'''
* '''Apr-09 Th'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Muscular exercise for training adipose tissue mitochondria'''
::::::: - from the O2k-Network Ā»[[DK Copenhagen Larsen S]]Ā«
::::::* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |Ā»Bioblast linkĀ«]]


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* '''Mar-11 We'''
* '''Apr-10 Fr'''
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. - Ā»'''[[Blebbistatin]]'''Ā«
Ā  2020 Week 16
Ā 
* '''Apr-13 Mo'''
Ā 
:{|
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Let's talk COVID-19 - a new culture of communication in science.''' - [https://www.sciencemag.org/news/2020/02/completely-new-culture-doing-research-coronavirus-outbreak-changes-how-scientists Kay Kupferschmidt in Science]
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - Ā»'''[[SUIT-024 |SUIT-024]]'''Ā«
|}
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* '''Apr-14 Tu'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - Ā»[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]Ā«
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::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while operating the O2k with biological sample?'''
* '''Apr-15 We''' Ā 
::::::: - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before [[uncoupler titrations]]. - Ā»[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]Ā«
Ā 
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* '''Mar-12 Th'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis'''
::::::: - from the O2k-Network Ā»[[UK London Duchen MR]]Ā«
::::::* Warne J, Pryce G, Hill J, Shi X, LennerĆ„s F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |Ā»Bioblast linkĀ«]]


* '''Apr-16 Th'''
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''
::::::: - from the O2k-Network Ā»[[DE Frankfurt Eckert GP]]Ā« Ā»[[CH Basel Eckert A]]Ā«
::::::* Leuner K, Schulz K, SchĆ¼tt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, MĆ¼ller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204.Ā  - [[Leuner 2012 Mol Neurobiol|Ā»Bioblast linkĀ«]]
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* '''Mar-13 Fr'''
* '''Apr-17 Fr'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Men and women with Type 1 diabetes and skeletal muscle mitochondria'''
::::::: - from the O2k-Network Ā»[[CA Toronto Perry CG]]Ā«
::::::* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab |Ā»Bioblast linkĀ«]]


2020 Week 17
* '''Apr-20 Mo'''


Ā  2020 Week 10
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''


* '''Mar-02 Mo'''
::::::: - from the O2k-Network Ā»[[US TN Nashville Wasserman DH]]Ā« and Ā»[[US MI East Lansing Bazil JN]]Ā«
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] An amazing number of new [[O2k-Publications: Topics |O2k-Publications]] from China. - Ā»'''[[O2k-Network Labs China]]'''Ā«
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |Ā»Bioblast linkĀ«]]
<br />
<br />


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction'''
::::::: - from the O2k-Network Ā»[[CN Chongqing Zhu Z]]Ā«
::::::* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |Ā»Bioblast linkĀ«]]


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Ā»[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]Ā«


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* '''Mar-03 Tu'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Rapamycin suppresses senescence in galactose versus glucose cell culture'''
::::::: - from the O2k-Network Ā»[[US PA Philadelphia Orynbayeva Z]]Ā«
::::::* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |Ā»Bioblast linkĀ«]]


* '''Apr-21 Tu'''
[[File:CS.png|right|100px|Citrate synthase]]
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Ā»'''[[MiPNet17.04 CitrateSynthase]]'''Ā«
|}
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* '''Apr-22 We'''


:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? - Ā»'''[[Rapamycin]]'''Ā«
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* '''Apr-23 Th''' Ā 


Ā 
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Advancing Open Science practices: stakeholder perspectives on incentives and disincentives.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Advancing Open Science practices |Ā»Bioblast linkĀ«]] - The concept of [[Gnaiger 2019 MitoFit Preprint Arch Editorial |Preprints]] needs full attention in Open Science.
* '''Apr-24 Fr'''
[[File:ETC-.JPG|right|150px|Electron transport chain]]
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]].
- Ā»'''[[Hatefi 1962 J Biol Chem-XLII]]'''Ā«
|}
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<br />
Ā  2020 Week 18


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* '''Apr-27 Mo''' Ā 
* '''Mar-04 We'''
:::: [[Image:O2k-Support.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''
[[File:Platelet.jpg|right|200px]]
::::::: The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub>Ā  concentrations. - Ā»[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]Ā«
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets''' Ā 
::::::: - from the O2k-Network Ā»[[US FL Orlando Goodpaster BH]]Ā«
::::::* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |Ā»Bioblast linkĀ«]] Ā 


[[File:Softdrink.jpg|right|100px]]
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Sugar and fat - initial mitochondrial aging in the brain'''
::::::: - from the O2k-Network Ā»[[IT Naples Iossa S]]Ā«
::::::* Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - [[Crescenzo 2019 Mol Neurobiol |Ā»Bioblast linkĀ«]]
[[File:OroboPOS-Holder002.jpg|right|100px]]
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - Ā»[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]Ā«
<br />


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* '''Apr-28 Tu'''
::::[[Image:O2k-Publications.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''
::::::: - from the O2k-Network Ā»[[FI Helsinki Jacobs HT]]Ā«, Ā»[[DE Magdeburg Gellerich FN]]Ā« and Ā»[[DE Magdeburg Debska-Vielhaber G]]Ā«
::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, LeƟmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - [[Szibor 2020 J Biol Chem|Ā»Bioblast linkĀ«]]


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* '''Mar-05 Th'''
::::* '''Abstract submission opens''' - Ā»[[Europhysiology 2020 Berlin DE]]Ā«


* '''Apr-29 We'''


:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] With a '''preprint''' you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - Ā»'''[[Preprint]]'''Ā«
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* '''Apr-30 Th'''




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* '''Mar-06 Fr'''
:::: [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''Explore metabolic profiles and specific modultations of mitochondrial pathways''' in health and disease with SUIT reference protocols. - Ā»'''[[SUIT-001 |RP1]]Ā« and Ā»[[SUIT-002 |RP2]]'''Ā«




== Feb 2020 ==
</div>
</div>


2020 Week 9
== Mar 2020 ==


* '''Feb-23 Su'''
[[File:Expand.png|right|35px |Click to expand or collaps]]
:::::::Ā» '''[[2020 PaduaMuscleDays Padova IT]]''' - ''postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy''


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<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">


* '''Feb-24 Mo'''
2020 Week 10
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] More research is needed on the impact of viral infection on mitochondrial function. - Ā»'''[[Viruses and mitochondrial medicine]]'''Ā«
<br />


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* '''Mar-02 Mo''' Ā 
* '''Feb-25 Tu'''
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] An amazing number of new [[O2k-Publications: Topics |O2k-Publications]] from China. - Ā»'''[[O2k-Network Labs China]]'''Ā«
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] High quality mitochondrial research is increasing rapidly in South Korea. - Ā»'''[[O2k-Network Labs Republic of Korea]]'''Ā«
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<br />
<br />
<br />


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''An active lifestyle protects cardiac mitochondria''' Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' Ā 
::::::: - from the O2k-Network: [[KR Busan Han J]], [[KR Incheon Kwak HB]]
::::::: - from the O2k-Network Ā»[[CN Chongqing Zhu Z]]Ā«
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |Ā»Bioblast linkĀ«]] Ā 
::::::* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |Ā»Bioblast linkĀ«]] Ā 


<br />


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[[File:2020-02-26 MitoEAGLE WG4 Innsbruck AT.jpg|right|400px|thumb|MitoEAGLE WG4 retreat; left to right: [[Garcia-Souza Luiz F |Luiz]], [[Gnaiger Erich |Erich]], [[Aasander Frostner Eleonor |Eleonor]], [[Michalak Slawomir |Slawomir]]]]
* '''Mar-03 Tu'''
* '''Feb-26 We''' Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Rapamycin suppresses senescence in galactose versus glucose cell culture''' Ā 
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. - Ā»'''[[Noncoupled respiration]]'''Ā«
::::::: - from the O2k-Network Ā»[[US PA Philadelphia Orynbayeva Z]]Ā«
<br />
::::::* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |Ā»Bioblast linkĀ«]]


:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] '''[[MitoEAGLE WG4 Innsbruck AT]]''' (Feb 26-28)


----
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? - Ā»'''[[Rapamycin]]'''Ā«
* '''Feb-27 Th'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase'''
::::::: - from the O2k-Network: [[CZ Prague Houstek J]]
::::::* Pajuelo-Reguera David, ČunĆ”tovĆ” KristĆ½na, VrbackĆ½ Marek, PecinovĆ” Alena, HouÅ”těk Josef, MrĆ”Äek TomĆ”Å”, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |Ā»Bioblast linkĀ«]] Ā 


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* '''Feb-28 Fr'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Ā»[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]Ā«


Ā 
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Advancing Open Science practices: stakeholder perspectives on incentives and disincentives.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Advancing Open Science practices |Ā»Bioblast linkĀ«]] - The concept of [[Gnaiger 2019 MitoFit Preprint Arch Editorial |Preprints]] needs full attention in Open Science.
2020 Week 8
<br />
Ā 
* '''Feb-17 Mo'''
:::: [[File:EBEClogo2020.jpg|80px|link=https://www.mitoglobal.org/index.php/EBEC2020_Marseille_FR]] '''Registration is open: [[EBEC2020_Marseille_FR |European Bioenergetics Conference 2020]]''', Marseille, France, 2020


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* '''Feb-18 Tu''' Ā 
* '''Mar-04 We'''
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? Ā» Dive deep into '''[[Oxygen solubility]]'''
[[File:Platelet.jpg|right|200px]]
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets'''
::::::: - from the O2k-Network Ā»[[US FL Orlando Goodpaster BH]]Ā«
::::::* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |Ā»Bioblast linkĀ«]]
Ā 
[[File:Softdrink.jpg|right|100px]]
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Sugar and fat - initial mitochondrial aging in the brain'''
::::::: - from the O2k-Network Ā»[[IT Naples Iossa S]]Ā«
::::::* Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - [[Crescenzo 2019 Mol Neurobiol |Ā»Bioblast linkĀ«]]
[[File:OroboPOS-Holder002.jpg|right|100px]]
<br />


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' Ā 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''
::::::: - from the O2k-Network Ā»[[US MI Ann Arbor Beard DA]]Ā«
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - Ā»[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]Ā«
::::::* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298ā€“314. - [[Tyrrell 2019 Circ Res |Ā»Bioblast linkĀ«]]
<br />




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* '''Feb-19 We''' Ā 
* '''Mar-05 Th'''
:::: [[Image:MitoFit Preprint Arch.png|80px|link=MitoFit Preprint Arch]] - Ā»'''[[MitoFit Preprint Arch]]'''Ā« was initiated one year ago. We invite submissions of mitochondrial preprints.
::::* '''Abstract submission opens''' - Ā»[[Europhysiology 2020 Berlin DE]]Ā«
Ā 


:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - Ā»'''[[Spermidine]]'''Ā«
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] With a '''preprint''' you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - Ā»'''[[Preprint]]'''Ā«




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* '''Feb-20 Th'''
* '''Mar-06 Fr'''
:::: [[Image:Logo MitoFit s.jpg|40px|link=MitoFit Open Seminars]] - '''[[MitoFit Open Seminars|MitoFit Open Seminar]]''': [[Stefan_E|Eduard Stefan]]: ā€œDecoding and Perturbation of ONCO-Kinase functionsā€ and [[Torres-Quesada_O|Omar Torres Quesada]]: ā€œ'''[[MitoKIN]]''' - Systematic barcoding of kinase-controlled mitochondrial functionā€
:::: [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''Explore metabolic profiles and specific modultations of mitochondrial pathways''' in health and disease with SUIT reference protocols. - Ā»'''[[SUIT-001 |RP1]]Ā« and Ā»[[SUIT-002 |RP2]]'''Ā«
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* '''Mar-08 Su''' - '''[https://www.internationalwomensday.com/ International Women's Day]'''


2020 Week 7
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Addressing the underrepresentation of women |Ā»Bioblast linkĀ«]]
<br />


*'''Feb-10 Mo'''
Ā  2020 Week 11
::::* '''Early Bird Registration Deadline''' for [[ESSR 2020 Innsbruck AT]]


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* '''Mar-09 Mo''' Ā 
*'''Feb-12 We''' Ā 
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] Ā»<big>'''[[MitoEAGLE WG2 Innsbruck AT]]'''</big>Ā« (Mar 09-13)
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] What do metformin, melatonin and other bioactive mitObesity compounds have in common? - Ā»'''[[MitObesity drugs]]'''Ā«




:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]''' participaing in [[MiPNet25.03 IOC144 Innsbruck AT]]
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Protein-specific mitochondrial respiratory capacity is higher in women than men''' Ā 
::::::: - from the O2k-Network Ā»[[SE Stockholm Cardinale DA]]Ā«
::::::* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |Ā»Bioblast linkĀ«]]




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*'''Feb-13 Th'''
* '''Mar-10 Tu'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Muscular exercise for training adipose tissue mitochondria'''
::::::: - from the O2k-Network Ā»[[DK Copenhagen Larsen S]]Ā«
::::::* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |Ā»Bioblast linkĀ«]]


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* '''Mar-11 We'''
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. - Ā»'''[[Blebbistatin]]'''Ā«


2020 Week 6


*'''Feb-03 Mo'''
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Let's talk COVID-19 - a new culture of communication in science.''' - [https://www.sciencemag.org/news/2020/02/completely-new-culture-doing-research-coronavirus-outbreak-changes-how-scientists Kay Kupferschmidt in Science]
:::[[File:FFG-logo.pngā€Ž|150px|FFG]] '''<big>Ā»Official start of the FFG funded project Ā»[[MitoKIN|'''MitoKIN''']]Ā«</big>'''Ā 
<br />


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' Ā 
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while operating the O2k with biological sample?'''
::::::: - from the O2k-Network Ā»[[US AR Little Rock Boersheim E]]Ā«
::::::: - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before [[uncoupler titrations]]. - Ā»[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]Ā«
::::::* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |Ā»Bioblast linkĀ«]]


::::* Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, [https://wiki.oroboros.at/index.php/Tremblay_N Dr Nelly Tremblay] is using for the first time the [[SUIT-022]] protocol to study alternative oxidases in the marine model organism ''Phaeodactylum tricornutum''.
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* '''Mar-12 Th'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis'''
::::::: - from the O2k-Network Ā»[[UK London Duchen MR]]Ā«
::::::* Warne J, Pryce G, Hill J, Shi X, LennerĆ„s F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |Ā»Bioblast linkĀ«]]


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* '''Mar-13 Fr'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Men and women with Type 1 diabetes and skeletal muscle mitochondria'''
::::::: - from the O2k-Network Ā»[[CA Toronto Perry CG]]Ā«
::::::* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab |Ā»Bioblast linkĀ«]]


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Ā  2020 Week 12Ā 
*'''Feb-04 Tu''' Ā 
* '''Mar-16 Mo''' Ā 
:::: [[Image:NextGenO2k logo.jpg|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.10 IOC and Distributor engagement Tokyo JP |'''MiPNet25.10 IOC and Distributor engagement Tokyo JP''']] (Feb 4-7)
::::: [[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|60px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]] '''Oroboros operates from now on in home office mode - we stay connected.'''
:::: [[File:O2k-brief.png|50px|link=https://wiki.oroboros.at/images/7/7b/Cronin_2018_Nature_O2k-brief.pdf|O2k-brief]] '''Cronin'''Ā  (2018)Ā  The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - '''Lymphocytes''', '''Cancer''' - [http://wiki.oroboros.at/index.php/O2k-brief O2k-brief repository]




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:::: [[Image:SUIT icon.jpg|80px|left|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''The standardized coupling control protocol to explore the bioenergetics of living cells''' Ā 
*'''Feb-05 We'''
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - Ā»'''[[SUIT-003 |SUIT-003]]'''Ā«
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimerā€™s disease'''
<br />
::::::: - from the O2k-Network Ā»[[US FL Boca Raton Khamoui AV]]Ā«
::::::* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |Ā»Bioblast linkĀ«]]
Ā 


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*'''Feb-06 Th'''
:::: '''MitoFit Open Seminar'''
:::::*Ā  16:00-16:30Ā  [[Beleza J|Jorge Beleza]]: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. [[Media:MitoFitOpenSeminar_JorgeBeleza_20200206.pdf|Ā»AbstractĀ«]]
:::::*Ā  16:30-17:00Ā  [[Pereira R|Rita Pereira]]: The Handball4Health Project : A community-based intervention programme


* '''Mar-17 Tu'''


== Jan 2020 ==
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ''' Zika virus infection leads to mitochondrial dysfunction'''
::::::: - from the O2k-Network Ā»[[BR Rio de Janeiro Galina A]]Ā«
::::::* Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - [[Ledur 2020 Sci Rep |Ā»Bioblast linkĀ«]]


2020 Week 5
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*'''Jan-27 Mo''' Ā 
* '''Mar-18 We''' Ā 
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.06 IOC145 Innsbruck AT |<big>'''IOC145 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 27-29)
Ā 
:::: DSL-Seminar, 16:00
:::::: [[Gnaiger E]]: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.
:::::: Univ.-Klinik fĆ¼r Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room


:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]Ā  '''Exercise protects against the effects of aging on mitochondrial function'''
::::::: - from the O2k-Network Ā»[[KR Busan Han J]]Ā« Ā»[[KR Incheon Kwak HB]]Ā«
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93.Ā  - [[No 2020 Pflugers Arch |Ā»Bioblast linkĀ«]]
<br />


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*'''Jan-28 Tu'''
* '''Mar-19 Th''' Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration of blood cells and cardiovascular disease''' Ā 
:::: [[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis '''Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home'''.
::::::: - from the O2k-Network Ā»[[FR Strasbourg Zoll J]]Ā«
::::::: - Ā» For the download link and password, please email [mailto:instruments@oroboros.at instruments@oroboros.at]
::::::* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |Ā»Bioblast linkĀ«]]
<br />
Ā 


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*'''Jan-30 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Karabatsiakis_A|Alexander Karabatsiakis]]: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.
* '''Mar-20 Fr'''
::::* '''Abstract submission deadline''' - Ā»[[SHVM 2020 Halifax CA]]Ā«
Ā  2020 Week 13


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''
* '''Mar-23 Mo''' Ā 
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - Ā»[[Oxygen calibration - DatLab]]Ā«


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ā€˜R1ā€™ and complete the air calibration correctly?'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''
::::::: '''(1) By adjusting the dithionite concentration toĀ  2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - Ā»'''[[Dithionite|Dithionite]]'''Ā« <br>
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - Ā»'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''Ā«


::::::: Your air calibration is not effective until you set mark ā€˜R1ā€™ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - Ā»[[Oxygen calibration - DatLab]]Ā«
Ā  Ā 


::::* '''Open Innovation''' in the NextGen-O2k software development DatLab 8. Here is a summary of [[MitoPedia: DatLab |MitoPedia]] pages including [[:Category:DatLab 8 perspectives |'''DatLab 8 perspectives''']]
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* '''Mar-24 Tu'''
:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection''' Ā 
::::::: - from the O2k-Network Ā»[[US NC Durham Li PA]]Ā«
::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |Ā»Bioblast linkĀ«]]
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* '''Mar-25 We'''
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''
::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? ā€“ An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]




:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā  [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: Ā»'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''Ā«
----
----
*'''Jan-31 Fr'''
* '''Mar-26 Th'''
::::* Deadline for [[APS2020_Chicago_US|APS2020]] Abstract submission for poster presentation.'''
::::* [[2020 PaduaMuscleDays Padova IT |Call for EJTM Communications]] - deadline for manuscript submissions
::::* Registration deadline for [[6th EU-Cardioprotection Meeting 2020 Riga LV]]


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''
----
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - Ā»[[Oxygen calibration - DatLab]]Ā«
* '''Mar-27 Fr'''
2020 Week 14


Ā  2020 Week 4
* '''Mar-30 Mo'''
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' Ā»'''[[Enable DL-Protocol editing]]'''Ā«


*'''Jan-20 Mo'''
----
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.03 IOC144 Innsbruck AT |<big>'''IOC144 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 20-22)
* '''Mar-31 Tu'''




----
*'''Jan-21 Tu'''
::::* Mr Hai Wu (CEO of [[CN Beijing Huawei |Huawei]], Oroboros distributor in China) and Dr. Rupert Ecker (CEO of [https://www.tissuegnostics.com/en/TissueGnostics Vienna TissueGnostics]) join the O2k-Coaching Days for extending collaborative projects.


</div>
</div>
== Feb 2020 ==


----
[[File:Expand.png|right|35px |Click to expand or collaps]]
*'''Jan-23 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Gnaiger E|Erich Gnaiger]]: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.


<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%">Ā  >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span>
<div class="mw-collapsible-content">
Ā  2020 Week 6


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*'''Feb-03 Mo''' Ā 
*'''Jan-24 Fr'''
:::[[File:FFG-logo.pngā€Ž|150px|FFG]] '''<big>Ā»Official start of the FFG funded project Ā»[[MitoKIN|'''MitoKIN''']]Ā«</big>'''Ā 
::::* Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - [[MitoEAGLE Summit Obergurgl 2020]]


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā  [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ā€˜O2k configurationā€™ window?'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' Ā 
::::::: Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - Ā»[[O2k configuration]]Ā«
::::::: - from the O2k-Network Ā»[[US AR Little Rock Boersheim E]]Ā«
::::::* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |Ā»Bioblast linkĀ«]]


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''
::::* Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, [https://wiki.oroboros.at/index.php/Tremblay_N Dr Nelly Tremblay] is using for the first time the [[SUIT-022]] protocol to study alternative oxidases in the marine model organism ''Phaeodactylum tricornutum''.
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - Ā»[[O2k-Chamber MitoPedia]]Ā«


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ā€˜O2 calibrationā€™ window in DatLab?
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - Ā»[[Oxygen solubility factor]]Ā«


2020 Week 3
----
Ā 
*'''Feb-04 Tu''' Ā 
*'''Jan-14 Tu''' Ā 
:::: [[Image:NextGenO2k logo.jpg|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.10 IOC and Distributor engagement Tokyo JP |'''MiPNet25.10 IOC and Distributor engagement Tokyo JP''']] (Feb 4-7)
::::* Circular ITC Grants for [[2020 PaduaMuscleDays Padova IT]], [[6th EU-Cardioprotection Meeting 2020 Riga LV]] and [[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]] - [https://wiki.oroboros.at/images/a/af/ITC_Newsletter.pdf pdf]
:::: [[File:O2k-brief.png|50px|link=https://wiki.oroboros.at/images/7/7b/Cronin_2018_Nature_O2k-brief.pdf|O2k-brief]] '''Cronin'''Ā  (2018)Ā  The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - '''Lymphocytes''', '''Cancer''' - [http://wiki.oroboros.at/index.php/O2k-brief O2k-brief repository]




----
----
*'''Jan-15 We''' Ā 
*'''Feb-05 We'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimerā€™s disease'''
::::::: - from the O2k-Network Ā»[[AU Melbourne Stepto NK]]Ā« Ā 
::::::: - from the O2k-Network Ā»[[US FL Boca Raton Khamoui AV]]Ā«
::::::* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - [[Jacques 2020 FASEB J |Ā»Bioblast linkĀ«]]
::::::* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |Ā»Bioblast linkĀ«]]




----
----
*'''Jan-16 Th'''
*'''Feb-06 Th'''
::::[[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Oxygen flux]]''' - which SI units should be used?
:::: '''MitoFit Open Seminar'''
Ā 
:::::*Ā  16:00-16:30Ā  [[Beleza J|Jorge Beleza]]: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. [[Media:MitoFitOpenSeminar_JorgeBeleza_20200206.pdf|Ā»AbstractĀ«]]
::::[[File:MitoPedia.jpg|60px|MitoPedia]] Did you ever think that units for body mass could be an issue? - Ā»'''[[Body mass]]'''Ā«
:::::*Ā  16:30-17:00Ā  [[Pereira R|Rita Pereira]]: The Handball4Health Project : A community-based intervention programme
Ā  2020 Week 7


*'''Feb-10 Mo'''
::::* '''Early Bird Registration Deadline''' for [[ESSR 2020 Innsbruck AT]]


----
----
*'''Jan-17 Fr''' Ā 
*'''Feb-12 We''' Ā 
::::[[Image:O2k-close.jpg|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]] <big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. - [[Scandurra_2010_Adv_Exp_Med_Biol |Ā»Bioblast linkĀ«]]
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] What do metformin, melatonin and other bioactive mitObesity compounds have in common? - Ā»'''[[MitObesity drugs]]'''Ā«


:::: [[Image:TRANSMIT Acronym.jpg|60px|TRANSMIT]] <big>'''[[TRANSMIT Symposium 2020 Brussels BE]]'''</big> (Jan 17-18)


:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]''' participaing in [[MiPNet25.03 IOC144 Innsbruck AT]]


::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations'''
::::::: - from the O2k-Network Ā»[[CZ Prague Krajcova A]]Ā«
::::::* KrajčovĆ” A, Urban T, Megvinet D, Waldauf P, BalĆ­k M, Hlavička J, Budera P, JanouÅ”ek L, PokornĆ” E, DuÅ”ka F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |Ā»Bioblast linkĀ«]]


2020 Week 2
----
*'''Feb-13 Th'''


*'''Jan-07 Tu''' The Oroboros Office opens in the New Year 2020
2020 Week 8
[[File:EBEClogo2022.jpg|right|300px|right|link=EBEC22 Innsbruck AT]]
::::* From the '''Austrian Bioenergetics Group''' to '''Mitochondrial Physiology and Bioenergetics DACH''' (D: Germany; A: Austria; CH: Switzerland)
:::::: [[EBEC22 Innsbruck AT |EBEC22 - the 22nd European Bioenergetics Conference]] will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the ''EBEC22 National Scientific Committee''. This provides an excellent opportunity to establish an ''Austrian Bioenergetics Group'' towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming [[1st Myocardial Function Symposium 2020 Graz AT]], and further at the [[MitoEAGLE Summit Obergurgl 2020]]. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at


* '''Feb-17 Mo'''
:::: [[File:EBEClogo2020.jpg|80px|link=https://www.mitoglobal.org/index.php/EBEC2020_Marseille_FR]] '''Registration is open: [[EBEC2020_Marseille_FR |European Bioenergetics Conference 2020]]''', Marseille, France, 2020


----
----
* '''Jan-08 We'''
* '''Feb-18 Tu''' Ā 
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? Ā» Dive deep into '''[[Oxygen solubility]]'''


:::: [[Image:Quality.png|left|60px|link=http://wiki.oroboros.at/index.php/MitoPedia:_Oroboros_QM |Quality]] Ā 
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis'''
::::::: - from the O2k-Network Ā»[[US MI Ann Arbor Beard DA]]Ā«
::::::* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298ā€“314. - [[Tyrrell 2019 Circ Res |Ā»Bioblast linkĀ«]]




----
----
* '''Jan-09 Th'''
* '''Feb-19 We''' Ā 
[[File:MITOEAGLE-representation.jpg|right|200px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]]
:::: [[Image:MitoFit Preprint Arch.png|80px|link=MitoFit Preprint Arch]] - Ā»'''[[MitoFit Preprint Arch]]'''Ā« was initiated one year ago. We invite submissions of mitochondrial preprints.
::::* Planning the [[MitoEAGLE Summit Obergurgl 2020]]
Ā 
:::::: Members of the [[Management Committee MitoEAGLE]] and [[MitoEAGLE Working Groups |Working Group Leaders]]: please submit your suggestions for [[MitoEAGLE Summit Obergurgl 2020]]:
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - Ā»'''[[Spermidine]]'''Ā«
::::::# Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output
::::::# Suggested scientists (titles) for presentations, according to the MoU




----
----
* '''Jan-10 Fr''' Ā 
* '''Feb-20 Th'''
::::Ā» <big>'''[[1st Myocardial Function Symposium 2020 Graz AT]]'''</big> (Jan 10-11)
:::: [[Image:Logo MitoFit s.jpg|40px|link=MitoFit Open Seminars]] - '''[[MitoFit Open Seminars|MitoFit Open Seminar]]''': [[Stefan_E|Eduard Stefan]]: ā€œDecoding and Perturbation of ONCO-Kinase functionsā€ and [[Torres-Quesada_O|Omar Torres Quesada]]: ā€œ'''[[MitoKIN]]''' - Systematic barcoding of kinase-controlled mitochondrial functionā€
::::* 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)




----
2020 Week 9
* '''Jan-12 So'''
::::* One month ago
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[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]
* '''Feb-23 Su'''
:: '''Cluster Awards 2019'''
:::::::Ā» '''[[2020 PaduaMuscleDays Padova IT]]''' - ''postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy''


<hr>
----


{|
* '''Feb-24 Mo'''
|[[File:Cluster Awards 2019.jpg|right|500px|Cluster Awards 2019]]
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] More research is needed on the impact of viral infection on mitochondrial function. - Ā»'''[[Viruses and mitochondrial medicine]]'''Ā«
On December 12<sup>th</sup>, the '''Cluster Awards 2019''' were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the '''Life Sciences Cluster''' for our EU Horizon 2020-funded '''[[NextGen-O2k |NextGen-O2k project]]'''.
<br />


'''Life Sciences'''
----
::: Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"
* '''Feb-25 Tu'''
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] High quality mitochondrial research is increasing rapidly in South Korea. - Ā»'''[[O2k-Network Labs Republic of Korea]]'''Ā«
<br />
<br />


'''Renewable Energies'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''An active lifestyle protects cardiac mitochondria''' Ā 
::: Heliotherm WƤrmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"
::::::: - from the O2k-Network: [[KR Busan Han J]], [[KR Incheon Kwak HB]]
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |Ā»Bioblast linkĀ«]]


'''Forest and wood'''
<br />
::: Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"


'''Information Technologies'''
----
::: Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"
[[File:2020-02-26 MitoEAGLE WG4 Innsbruck AT.jpg|right|400px|thumb|MitoEAGLE WG4 retreat; left to right: [[Garcia-Souza Luiz F |Luiz]], [[Gnaiger Erich |Erich]], [[Aasander Frostner Eleonor |Eleonor]], [[Michalak Slawomir |Slawomir]]]]
* '''Feb-26 We''' Ā 
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. - Ā»'''[[Noncoupled respiration]]'''Ā«
<br />


'''Mechatronics''': ALPEX Technologies GmbH, Mils: "EVEREST 4.0"
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] '''[[MitoEAGLE WG4 Innsbruck AT]]''' (Feb 26-28)


'''Wellness''': Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"
----
* '''Feb-27 Th'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase'''
::::::: - from the O2k-Network: [[CZ Prague Houstek J]]
::::::* Pajuelo-Reguera David, ČunĆ”tovĆ” KristĆ½na, VrbackĆ½ Marek, PecinovĆ” Alena, HouÅ”těk Josef, MrĆ”Äek TomĆ”Å”, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |Ā»Bioblast linkĀ«]]


Ā» More details (in German): '''[https://www.standort-tirol.at/page.cfm?vpath=newsroom/news&genericpageid=26328 Standortagentur Tirol]'''.
----
<div style="text-align: right; direction: ltr; margin-left: 1em;">Photo Ā© Standortagentur Tirol</div>
* '''Feb-28 Fr'''
|}
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''
|}
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Ā»[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]Ā«
Ā 
Ā 
Ā 
</div>
</div>
Ā 
== Jan 2020 ==
[[File:Expand.png|right|35px |Click to expand or collaps]]


Ā  2020 Week 1 '''The Oroboros team enjoys ''holidays'' from 2019 Dec 24 to 2020 Jan 06'''
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Ā  2020 Week 1 '''The Oroboros team enjoys ''holidays'' from 2019 Dec 24 to 2020 Jan 06'''
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Ā  2020 Week 2
*'''Jan-07 Tu''' The Oroboros Office opens in the New Year 2020
[[File:EBEClogo2022.jpg|right|300px|right|link=EBEC22 Innsbruck AT]]
::::* From the '''Austrian Bioenergetics Group''' to '''Mitochondrial Physiology and Bioenergetics DACH''' (D: Germany; A: Austria; CH: Switzerland)
:::::: [[EBEC22 Innsbruck AT |EBEC22 - the 22nd European Bioenergetics Conference]] will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the ''EBEC22 National Scientific Committee''. This provides an excellent opportunity to establish an ''Austrian Bioenergetics Group'' towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming [[1st Myocardial Function Symposium 2020 Graz AT]], and further at the [[MitoEAGLE Summit Obergurgl 2020]]. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at
----
* '''Jan-08 We'''
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.
:::: [[Image:Quality.png|left|60px|link=http://wiki.oroboros.at/index.php/MitoPedia:_Oroboros_QM |Quality]]
----
* '''Jan-09 Th'''
[[File:MITOEAGLE-representation.jpg|right|200px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]]
::::* Planning the [[MitoEAGLE Summit Obergurgl 2020]]
:::::: Members of the [[Management Committee MitoEAGLE]] and [[MitoEAGLE Working Groups |Working Group Leaders]]: please submit your suggestions for [[MitoEAGLE Summit Obergurgl 2020]]:
::::::# Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output
::::::# Suggested scientists (titles) for presentations, according to the MoU
----
* '''Jan-10 Fr'''
::::Ā» <big>'''[[1st Myocardial Function Symposium 2020 Graz AT]]'''</big> (Jan 10-11)
::::* 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)
----
* '''Jan-12 So'''
::::* One month ago
{| style="width:95%;
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<table>
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]
:: '''Cluster Awards 2019'''
<hr>
{|
|[[File:Cluster Awards 2019.jpg|right|500px|Cluster Awards 2019]]
On December 12<sup>th</sup>, the '''Cluster Awards 2019''' were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the '''Life Sciences Cluster''' for our EU Horizon 2020-funded '''[[NextGen-O2k |NextGen-O2k project]]'''.
'''Life Sciences'''
::: Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"
'''Renewable Energies'''
::: Heliotherm WƤrmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"
'''Forest and wood'''
::: Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"
'''Information Technologies'''
::: Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"
'''Mechatronics''': ALPEX Technologies GmbH, Mils: "EVEREST 4.0"
'''Wellness''': Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"
Ā» More details (in German): '''[https://www.standort-tirol.at/page.cfm?vpath=newsroom/news&genericpageid=26328 Standortagentur Tirol]'''.
<div style="text-align: right; direction: ltr; margin-left: 1em;">Photo Ā© Standortagentur Tirol</div>
|}
|}
Ā  2020 Week 3
*'''Jan-14 Tu'''
::::* Circular ITC Grants for [[2020 PaduaMuscleDays Padova IT]], [[6th EU-Cardioprotection Meeting 2020 Riga LV]] and [[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]] - [https://wiki.oroboros.at/images/a/af/ITC_Newsletter.pdf pdf]
----
*'''Jan-15 We'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers'''
::::::: - from the O2k-Network Ā»[[AU Melbourne Stepto NK]]Ā«
::::::* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - [[Jacques 2020 FASEB J |Ā»Bioblast linkĀ«]]
----
*'''Jan-16 Th'''
::::[[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Oxygen flux]]''' - which SI units should be used?
::::[[File:MitoPedia.jpg|60px|MitoPedia]] Did you ever think that units for body mass could be an issue? - Ā»'''[[Body mass]]'''Ā«
----
*'''Jan-17 Fr'''
::::[[Image:O2k-close.jpg|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]] <big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. - [[Scandurra_2010_Adv_Exp_Med_Biol |Ā»Bioblast linkĀ«]]
:::: [[Image:TRANSMIT Acronym.jpg|60px|TRANSMIT]] <big>'''[[TRANSMIT Symposium 2020 Brussels BE]]'''</big> (Jan 17-18)
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations'''
::::::: - from the O2k-Network Ā»[[CZ Prague Krajcova A]]Ā«
::::::* KrajčovĆ” A, Urban T, Megvinet D, Waldauf P, BalĆ­k M, Hlavička J, Budera P, JanouÅ”ek L, PokornĆ” E, DuÅ”ka F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |Ā»Bioblast linkĀ«]]
2020 Week 4
*'''Jan-20 Mo'''
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.03 IOC144 Innsbruck AT |<big>'''IOC144 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 20-22)
----
*'''Jan-21 Tu'''
::::* Mr Hai Wu (CEO of [[CN Beijing Huawei |Huawei]], Oroboros distributor in China) and Dr. Rupert Ecker (CEO of [https://www.tissuegnostics.com/en/TissueGnostics Vienna TissueGnostics]) join the O2k-Coaching Days for extending collaborative projects.
----
*'''Jan-23 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Gnaiger E|Erich Gnaiger]]: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.
----
*'''Jan-24 Fr'''
::::* Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - [[MitoEAGLE Summit Obergurgl 2020]]
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā  [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ā€˜O2k configurationā€™ window?'''
::::::: Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - Ā»[[O2k configuration]]Ā«
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - Ā»[[O2k-chamber]]Ā«
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ā€˜O2 calibrationā€™ window in DatLab?
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - Ā»[[Oxygen solubility factor]]Ā«
2020 Week 5
*'''Jan-27 Mo'''
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.06 IOC145 Innsbruck AT |<big>'''IOC145 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 27-29)
:::: DSL-Seminar, 16:00
:::::: [[Gnaiger E]]: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.
:::::: Univ.-Klinik fĆ¼r Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room
----
*'''Jan-28 Tu'''
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration of blood cells and cardiovascular disease'''
::::::: - from the O2k-Network Ā»[[FR Strasbourg Zoll J]]Ā«
::::::* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311.Ā  - [[Alfatni 2020 J Clin Med |Ā»Bioblast linkĀ«]]
----
*'''Jan-30 Th'''Ā  - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Karabatsiakis_A|Alexander Karabatsiakis]]: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - Ā»[[Oxygen calibration - DatLab]]Ā«
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ā€˜R1ā€™ and complete the air calibration correctly?'''
::::::: Your air calibration is not effective until you set mark ā€˜R1ā€™ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - Ā»[[Oxygen calibration - DatLab]]Ā«
Ā  Ā 
::::* '''Open Innovation''' in the NextGen-O2k software development DatLab 8. Here is a summary of [[MitoPedia: DatLab |MitoPedia]] pages including [[:Category:DatLab 8 perspectives |'''DatLab 8 perspectives''']]
----
*'''Jan-31 Fr'''
::::* Deadline for [[APS2020_Chicago_US|APS2020]] Abstract submission for poster presentation.'''
::::* [[2020 PaduaMuscleDays Padova IT |Call for EJTM Communications]] - deadline for manuscript submissions
::::* Registration deadline for [[6th EU-Cardioprotection Meeting 2020 Riga LV]]
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - Ā»[[Oxygen calibration - DatLab]]Ā«




</div>
</div>
== Stay alert ==
== Stay alert ==
{{Oroboros Ecosystem Agenda list}}
{{Oroboros Ecosystem Agenda list}}

Revision as of 08:13, 30 November 2020

                



Oroboros Ecosystem Agenda 2020 Archive

Oroboros Ecosystem Agenda 2020
Mitochondria and Cell Research - the Oroboros Ecosystem
Ā» Current Oroboros Ecosystem Agenda 2020

Last week

2020 Week 48
  • Nov-23 Mo
O2k-Open Support Take care Cell culture medium and dithionite titrations ā€“ a bad combination for an instrumental O2 background test?

Cell culture media are not ideal for instrumental O2 background test when using dithionite titrations because side reactions affecting the O2 concentration and thus interfering with the test. Instead of cell culture media, use MiR05, MiR06 or a strongly buffered alkaline phosphate solution.
-Ā»MiPNet14.06 Instrumental O2 backgroundĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cecatto CristianeĀ« 


  • Nov-24 Tu
ISCaM 2020 Virtual Event

  • Nov-25 We
O2k-Publications in the MiPMap Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.
from the O2k-Network Ā»CA Guelph Holloway GPĀ«
  • Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. Ā»Bioblast linkĀ«

  • Nov-26 Th

  • Nov-27 Fr
O2k-brief repository HSP60 knockdown and its effect on mitochondriaā€™s role in pancreatic ductal adenocarcinoma
  • Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161. Ā»Bioblast linkĀ«

Nov 2020

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2020 Week 45
  • Nov-02 Mo
MitoPedia
How can you measure mitochondrial membrane potential with O2k-Fluorometry?

The extrinsic fluorophores safranin, TMRM or Rhodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations.
- Ā»SafraninĀ«, Ā»TMRMĀ«, Ā»Rhodamine 123Ā« 
- communicated by Ā»Komlodi TimeaĀ«, Ā»Iglesias-Gonzalez JavierĀ« and Ā»Cecatto CristianeĀ«



  • Nov-04 We
O2k-Publications in the MiPMap Six-month caloric restriction causes tissue-specific bioenergetic changes in mitochondria isolated from rat heart and skeletal muscle
from the O2k-Network Ā»BR Sao Paulo Kowaltowski AJĀ«
  • Serna JDC, Caldeira da Silva CC, Kowaltowski AJ (2020) Functional changes induced by caloric restriction in cardiac and skeletal muscle mitochondria. J Bioenerg Biomembr 52:269-77.Ā»Bioblast linkĀ«

  • Nov-05 Th

  • Nov-06 Fr
Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event
2020 Week 46
O2k-Open SupportTake care Have you observed a high signal at zero oxygen?

A high signal at zero oxygen might be due to a technical issue with the O2k-Main Unit, the OroboPOS-Connector, the OroboPOS or a bad electrical connection between these components. The high zero current test helps you to locate the cause.
- Ā»High signal at zero oxygenĀ« 
- communicated by Ā»Schmitt SabineĀ« and Ā»Komlodi TimeaĀ«


  • Nov-10 Tu

  • Nov-11 We

  • Nov-12 Th

  • Nov-13 Fr

  • Nov-14 Sa - World Diabetes Day
O2k-Publications in the MiPMap Gastric bypass surgery improves diabetic nephropathy by reducing NADPH production via restoration of AMPK activity in type 1 diabetic rats
from the O2k-Network Ā»CN Chongqing Zhu ZĀ«
  • Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207. Ā»Bioblast linkĀ«
2020 Week 47
  • Nov-16 Mo
O2k-Publications in the MiPMap Mitochondrial function is impaired in human iPSC-derived astrocytes infected with Zika virus.
from the O2k-Network Ā»BR Rio de Janeiro Galina AĀ« and Ā»BR Rio de Janeiro Institute Biomedical ChemistryĀ«
  • Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. Ā»Bioblast linkĀ«


  • Nov-18 We

  • Nov-19 Th
Ā» 2020 PaduaMuscleDays Padua IT / IOC Padua IT
MitoPedia
What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?

For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- Ā»Optimum ADP concentration in pfiĀ« 
- Ā» Komlodi TimeaĀ« and Ā» Doerrier CarolinaĀ« 


  • Nov-20 Fr
2020 PaduaMuscleDays Padua IT
O2k-Events
Dr. Carolina Doerrier and Dr. Erich Gnaiger will be presenting at the virtual 2020 PaduaMuscleDays Padua IT
  • 09:30 - Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers - Ā»IOC Padua ITĀ« 
  • 15:40 - Body mass excess, muscle mass, obesity and mitochondrial fitness

  • Nov-21 Sa
2020 PaduaMuscleDays Padua IT

Oct 2020

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>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

  • Oct-01 Th
Abstract submission deadline for: Integrative Physiology of Exercise 2020 Virtual Event

  • Oct-02 Fr


2020 Week 41
  • Oct-05 Mo
    O2kHandOver 20201002.jpg
FFG Dr. Markus Keller and his team have now received the O2ks for their measurements in the FFG-funded TissueModels project!

The project aims to establish physiological models for specific tissue types. Oroboros Instruments is a consortium partner, providing infrastructure and expertise.

- Read more Ā»TissueModelsĀ«


O2k-Publications in the MiPMap Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?
from the O2k-Network Ā»BR Sao Paulo Kowaltowski AJĀ«
  • Menezes-Filho SL, Amigo I, LuĆ©vano-MartĆ­nez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. Ā»Bioblast linkĀ«

  • Oct-07 We
MitoPedia
Background H2O2 calibration: what is it and why perform it when using Amplex UltraRed assay?

Background H2O2 calibration refers to a set of H2O2 titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay.
- Ā»Background H2O2 calibrationĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Schmitt SabineĀ« 


  • Oct-08 Th
O2k-Publications in the MiPMap Childhood maltreatment is associated with increased mitochondrial bioenergetic function of immune cells in mothers, but not in their newborns
from the O2k-Network Ā»DE Ulm Gumpp AĀ«
  • Gumpp AM, Boeck C, Behnke A, Bach AM, Ramo-FernĆ”ndez L, Welz T, GĆ¼ndel H, Kolassa IT, Karabatsiakis A (2020) Childhood maltreatment is associated with changes in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84. Ā»Bioblast linkĀ«

  • Oct-09 Fr
Ā»Long Night of Research 2020 Innsbruck ATĀ«


2020 Week 42
O2k-Open SupportInfo: improve If using the Amplex UltraRed assay, when should the background H2O2 calibration be performed?

Background H2O2 calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H2O2 is prepared, or if a different respiration medium is used. As the H2O2 stock solution needs to be freshly prepared, it is recommended to perform a background H2O2 calibration before each experiment and at a minimum once per experimental day.
- Ā»How to find the background H2O2 calibration protocol in DatLab Ā« select ā€œAmR calibrationā€ for the background H2O2 calibration protocol
- communicated by Ā»Komlodi TimeaĀ« and Ā»Schmitt SabineĀ« 


  • Oct-13 Tu
FAT4BRAIN Ā»FAT4BRAIN School IOC147 Virtual EventĀ«

  • Oct-14 We
O2k-Publications in the MiPMap Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction?
from the O2k-Network Ā»US VA Richmond Lesnefsky EJĀ« 
  • Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. Ā»Bioblast linkĀ«

  • Oct-15 Th
MitoPedia
How can you reduce the amount of biological sample needed for high-resolution respirometry?

The O2k-sV-Module provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.
- Ā»O2k-sV-ModuleĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Schmitt SabineĀ« 


  • Oct-16 Fr
MitoPedia: SUIT Explore mitochondrial pathways inPBMC and platelets

The following SUIT protocols are specifically optimized for PBMC and platelets
- Ā»SUIT-001 O2 ce-pce D004Ā« and Ā»SUIT-002 O2 ce-pce D007aĀ« 
- communicated by Ā»Cecatto CristianeĀ« and Ā»Garcia-Souza Luiz FĀ« 


  • Oct-17 Sa

  • Oct-18 Su
Ā»ISCaM 2020 Virtual EventĀ«
 2020 Week 43
  • Oct-19 Mo
O2k-Open SupportInfo: improve Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?

Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 ĀµM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: Ā»Krumschnabel 2014 Methods EnzymolĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cecatto CristianeĀ«



  • Oct-21 We
O2k-Publications in the MiPMap Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies
from the O2k-Network Ā»US NC Winston-Salem Molina AJAĀ«
  • Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.Ā»Bioblast linkĀ«
MitoPedia
Human PBMC and Platelets - samples for high-resolution respirometry obtained by minimally invasive procedures.

We compared isolation procedures for blood cells.
- Ā»MiPNet21.17 Blood Cells IsolationĀ« 
- communicated by Ā»Cecatto CristianeĀ« and Ā»Garcia-Souza Luiz FĀ« 


  • Oct-22 Th
Bioenergetics Communications
LEAK respiration relates to intrinsic uncoupling.

Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.
- Ā»BEC2020.1: Mitochondrial physiologyĀ« - Table 2. Terms on respiratory coupling and uncoupling
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cecatto CristianeĀ« and Ā»Gnaiger ErichĀ«


  • Oct-23 Fr
O2k-brief repository Simultaneous measurement of O2 flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system
from the O2k-Network Ā»AT Innsbruck OroborosĀ« and Ā»AT Innsbruck Gnaiger EĀ«
  • Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. Ā»Bioblast linkĀ«
2020 Week 44
  • Oct-26 Mo
TRANSMIT
"TRANSMIT: THE ROAD TO MEET THE MITOS" ā€“ the interactive TRANSMIT booklet for children is available for download in multiple languages

- Ā»https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/

O2k-Open SupportInfo: improve Did you know that oxygen flux can be converted to flux control ratios (FCRs) directly in DatLab?

Use this function to easily plot your experimental traces as FCRs, or to analyze your data.
- Ā»FCR in DatLabĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cecatto CristianeĀ«


  • Oct-27 Tu
O2k-Network
Gentle Science
The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab
from the O2k-Network Ā»CH Lausanne Place NĀ« 
  • Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. Ā»Bioblast linkĀ«

  • Oct-28 We
Bioenergetics Communications
Flow or flux - what is the difference?

Rate can be expressed both as flow (I) and flux (J). Flow is used if the sample X can be quantified as count (NX; the number of cells, organisms), and changes proportionally with the size of X. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.
- Ā»BEC2020.1: Mitochondrial physiologyĀ« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalar
- communicated by Ā»Cecatto CristianeĀ« and Ā»Komlodi TimeaĀ« and Ā»Gnaiger ErichĀ«


  • Oct-29 Th
MitoPedia
Would you like to monitor O2 flux in response to light?

Read about the PB-Module, the new module in the NextGen-O2k that will enable experiments in PhotoBiology!
- Ā»PB-ModuleĀ«, Ā»PhotoBiologyĀ« 
- communicated by Ā»Huete-Ortega MariaĀ« and Ā»Schmitt SabineĀ« and Ā»Tindle-Solomon LisaĀ«


  • Oct-30 Fr
O2k-brief repository Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)
from the O2k-Network Ā»HU Budapest Chinopoulos CĀ« 
  • Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. Ā»Bioblast linkĀ«

Sep 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

2020 Week 36
  • Sep-01 Tu
O2k-Publications in the MiPMap A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction
from the O2k-Network Ā»US PA Philadelphia Kilbaugh TĀ«, Ā»US PA Philadelphia Jang DHĀ« and Ā»SE Lund Elmer EĀ«
  • Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. Ā»Bioblast linkĀ«

  • Sep-02 We



  • Sep-03 Th

  • Sep-04 Fr

  • Sep-05 Sa

  • Sep-06 So
2020 Week 37
  • Sep-07 Mo
O2k-Open Support Take care Why it is necessary to perform multiple H2O2 calibrations when using the Amplex UltraRed (AmR) assay?

The sensitivity to H2O2 of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H2O2 calibration before sample addition, after sample addition and multiple times during the experiment.
- Ā»Komlodi 2018 Methods Mol BiolĀ« 

The DLPs for AmR SUIT protocols already come with multiple steps of H2O2 calibrations implemented.
- Ā» SUITbrowserĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cardoso LuizaĀ«


  • Sep-08 Tu
O2k-Publications in the MiPMap Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung
from the O2k-Network Ā»DE Giessen Weissmann NĀ«, Ā»FI Tampere Dufour EĀ«, Ā»FI Helsinki Jacobs HTĀ« and Ā»DE Jena Szibor MĀ«
  • Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. Ā»Bioblast linkĀ«

  • Sep-09 We

  • Sep-10 Th
MitoPedia
What is the limitation of State 3 respiration compared to OXPHOS capacity?

OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, Pi, fuel substrates and O2. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of excess E-P capacity due to kinetic limitations of the phosphorylation system.
- Ā»BEC: Mitochondrial physiologyĀ« - Table 3 and several figures.
- communicated by Ā» Cecatto Cristiane Ā« and Ā» Komlodi Timea Ā«



  • Sep-11 Fr
2020 Week 38
  • Sep-14 Mo
MitoGlobal Events World Mitochondrial Disease Week ā€“ Sep 13-19

World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.

See what is happening near you - Ā»https://mitochondrialdiseaseweek.org/events/


  • Sep-15 Tu - World Lymphoma Awareness Day
O2k-Publications in the MiPMap OXPHOS as a potential therapeutic target in classical Hodgkin lymphoma
from the O2k-Network Ā»DE Frankfurt Droese SĀ«, Ā»DE Frankfurt Wittig IĀ« and Ā»NL Nijmegen Brandt UĀ«
  • Birkenmeier K, Droese S, Wittig I, Winkelmann R, KƤfer V, Doering C, Hartmann S, Wenz T, Reichert AS, Brandt U, Hansmann ML (2016) Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma are highly dependent on oxidative phosphorylation. Int J Cancer 138:2231-46. Ā»Bioblast linkĀ«

  • Sep-16 We
MitoPedia
Why are permeabilized muscle fibers (pfi) a poor sample type for studying reactive oxygen species (ROS) production?

In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation. However, the high oxygen pressures used may artificially increase ROS, including H2O2 production, making pfi a less optimal model for ROS production studies.
- Ā»Oxygen dependence of ROS production in pfiĀ« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cardoso LuizaĀ«


  • Sep-17 Th
MitoPedia: SUIT Did you know that you can measure the respiratory activity available for phosphorylation of ADP to ATP in living cells?

SUIT protocols from the SUIT-003 family will guide you through the titrations needed to analyze free ROUTINE activity.
- Ā»SUIT-003 O2 ce D009Ā« and Ā»SUIT-003 O2 ce-pce D020Ā« 
- communicated by Ā»Cardoso LuizaĀ« and Ā»Komlodi TimeaĀ«


  • Sep-18 Fr

  • Sep-19 Sa

  • Sep-20 So
ESCI.JPG ESCI 2020 Virtual Event Sep 20-30

2020 Week 39
ƖGMBT Graz AT 12th ƖGMBT Annual Meeting 2020 Virtual Event Sep 21-23

- World Alzheimer's Day

O2k-Publications in the MiPMap Olive polyphenols prove promising in the protection against mitochondrial dysfunction due to Alzheimer's disease and brain aging
from the O2k-Network Ā»DE Giessen Eckert GPĀ«
  • Grewal R, Reutzel M, Dilberger B, Hein H, Zotzel J, Marx S, Tretzel J, Sarafeddinov A, Fuchs C, Eckert GP (2020) Purified oleocanthal and ligstroside protect against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing. Exp Neurol 328:113248. Ā»Bioblast linkĀ«
O2k-brief repository Resistance training effects in Alzheimerā€™s disease mouse model
from the O2k-Network Ā»US FL Boca Raton Khamoui AVĀ« 
  • Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Dis 2020:Article ID 5919501. Ā»Bioblast linkĀ«

MitoPedia: SUIT Did you know the reverse electron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using Amplex UltraRed assay?

The reverse electron flow, also called reverse electron transfer (RET), happens when electrons are transferred from Complex II to the Q-junction and then back to Complex I at high mt-membrane potential. This phenomenon results in high hydrogen peroxide production in some mitochondrial preparations. The SUIT-026 protocol was designed to investigate this effect.
- Ā»SUIT-026Ā« 
- communicated by Ā»Komlodi TimeaĀ« and Ā»Cardoso LuizaĀ« 




2020 Week 40
O2k-Open SupportInfo: improve Are whitish deposits forming on the glass wall of the O2k-Chamber?

These deposits might be an accumulation of proteins and subsequent embedding of other biological material or small glass splinters, and a sign that the O2k-Chamber requires cleaning.
For further details on how to clean the O2k-Chamber properly, see:
- Ā»Cleaning the O2k-chamberĀ«
- communicated by Ā»Komlodi TimeaĀ«, Ā»Sabine SchmittĀ«, and Ā»Lisa Tindle-SolomonĀ«


World Heart Day
O2k-Publications in the MiPMap Succinate dehydrogenase inhibition protects myocardium from ischemia reperfusion injury but efficacy may be influenced by diabetes
from the O2k-Network Ā»DK Aarhus Boetker HEĀ« and Ā»DK Copenhagen Larsen SĀ«
  • Jespersen NR, Hjortbak MV, Lassen TR, StĆøttrup NB, Johnsen J, Tonnesen PT, Larsen S, Kimose HH, BĆøtker HE (2020) Cardioprotective effect of succinate dehydrogenase inhibition in rat hearts and human myocardium with and without diabetes mellitus. Sci Rep 10:10344. Ā»Bioblast linkĀ«
Projects As a Horizon 2020 beneficiary, Oroboros signed the Manifesto for EU COVID-19 Research, showing our support for the accessibility of research results in the fight against COVID-19.



Aug 2020

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2020 Week 32
  • Aug-03 Mo
O2k-Open SupportInfo: improve What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?

It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings.
- Ā» Troubleshooting for problems with text in DatLabĀ«


  • Aug-04 Tu
O2k-Publications in the MiPMap Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system
from the O2k-Network Ā»US PA Pittsburgh Jurczak MJĀ«
  • Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. Ā»Bioblast linkĀ«

  • Aug-05 We

  • Aug-06 Th
MitoPedia
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system. This coupling control factor ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (L=P). It is suggested to use OXPHOS-coupling efficiency instead of the respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.

- Ā»OXPHOS-coupling efficiencyĀ«


  • Aug-07 Fr
2020 Week 33

  • Aug-10 Mo

  • Aug-11 Tu
O2k-Publications in the MiPMap Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring
from the O2k-Network Ā»DE Bremerhaven Mark FCĀ«
  • Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. Ā»Bioblast linkĀ«

  • Aug-12 We
MitoFit pdf Some terms in ā€œMitochondrial physiologyā€ Bioenergetics Communications 2020.1 (States and rates) did not look familiar ā€• what is the elementary unit [x]?

Fundamental answers, a critical discussion on the SI unit for count, and the meaning of Euclidā€™s unit and Euclidā€™s number are presented in an unusual ā€˜canonical reviewā€™:


Bioenergetics Communications Join the discussion on the MitoEAGLE Consortium Communication: ā€œMitochondrial physiologyā€ Bioenergetics Communications 2020.1

Ā» On a Comment in Nature Metabolism Ā«


  • Aug-13 Th
MitoPedia
Photosynthesis efficiency is reduced by 25 % in plants and algae due the metabolic process called photorespiration.

- Ā»PhotorespirationĀ«


  • Aug-14 Fr
O2k-brief repository Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis
from the O2k-Network Ā»NZ Auckland Hickey AJĀ« 
  • Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. Ā»Bioblast linkĀ«
2020 Week 34
  • Aug-17 Mo
  • Aug-18 Tu
O2k-Publications in the MiPMap Mitochondrial respiration in PBMCs from patients with major depression
from the O2k-Network Ā»DE Ulm Karabatsiakis AĀ« and Ā»DE Ulm Radermacher PĀ«
  • Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. Ā»Bioblast linkĀ«

  • Aug-19 We

  • Aug-20 Th
MitoPedia
Would you like to simultaneously monitor O2 flux and the redox state of the Q-junction in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the H2020 SME NextGen-O2k project:


- Ā»Q-ModuleĀ« 


  • Aug-21 Fr
2020 Week 35
  • Aug-24 Mo
MitoPedia
Can NADH be used as an externally added substrate to obtain the NADH electron transfer-pathway state in mitochondrial respiration?

No, in most types of intact mitochondria NADH cannot pass the mitochondrial inner membrane (mtIM). Yeast mitochondria are an exception.
- Ā»Nicotinamide adenine dinucleotideĀ« and Ā»Substrates and metabolitesĀ« 


  • Aug-25 Tu
O2k-Publications in the MiPMap Low-intensity exercise increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults
from the O2k-Network Ā»LV Riga Makrecka-Kuka MĀ«
  • Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. ā€” Ā»Bioblast linkĀ«

  • Aug-26 We
MitoPedia
Non-mitochondrial respiration or residual oxygen consumption (Rox)? ā€” Concept-driven constructive terminology frames our perception.

After inhibition of electron-transfer (ET) pathways or in the absence of fuel substrates, both mitochondrial non-ET reactions and non-mitochondrial reactions contribute to residual oxygen consumption in mt-preparations and living cells.
- Ā»BEC: Mitochondrial physiologyĀ« ā€” Table 1: Rox induced by non-ET pathway oxidation reactions


  • Aug-27 Th

  • Aug-28 Fr


Jul 2020

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 2020 Week 27
  • Jul-01 We
TRANSMIT Kick-off of the TRANSMIT crowdfunding campaign: NOW
Bring the world of scientific research closer to the world of patients by supporting four cancer patientsā€™ associations.
- Ā»https://www.ideaginger.it/progetti/now.htmlĀ«

  • Jul-02 Th
MitoPedia
Did you know that if ET capacity E.jpg is lower than OXPHOS capacity P.jpg (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:

- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity.

Information from: Gnaiger Erich et al ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»Bioblast linkĀ« 


  • Jul-03 Fr
O2k-brief repository Mitochondrial H2O2 flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration
from the O2k-Network Ā»US CO Fort Collins Chicco AJĀ« 
  • Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. Ā»Bioblast linkĀ«

2020 Week 28 
  • Jul-06 Mo
O2k-Open SupportWarning: loss of data Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature?

During air calibration, temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the temperature control, an error message will appear. For more information, see:
- Ā»Block temperature does not increase while heating with full powerĀ« and Ā»DatLab error messages Ā«


  • Jul-07 Tu
O2k-Publications in the MiPMap Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice
from the O2k-Network Ā»FR Lille Duez HĀ«
  • Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. Ā»Bioblast linkĀ«

  • Jul-08 We

  • Jul-09 Th
MitoPedia
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- Ā»Setting the oxygen concentrationĀ«


  • Jul-10 Fr
 2020 Week 29
  • Jul-13 Mo
O2k-Open SupportInfo: improve Did you know that you can apply instrumental O2 background test parameters (i.e., intercept and slope) from an existing file in DatLab?

In the DatLab file, you can copy the Instrumental O2 background test parameters directly via the ā€˜Copy from fileā€™ option in the ā€˜Slope configurationā€™ window. For more information, see: - Ā»Copy from fileĀ«


  • Jul-14 Tu
O2k-Publications in the MiPMap Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured Jatropha curcas cells
from the O2k-Network Ā»BR Ribeirao Preto Alberici LCĀ«
  • Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on Jatropha curcas cell culture. Plant Physiol Biochem 136:92-97. Ā»Bioblast linkĀ«

  • Jul-15 We



  • Jul-16 Th
MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- Ā»Selecting the Smart Fluo-SensorĀ« and Ā»Selecting a Filter SetĀ«


  • Jul-17 Fr

2020 Week 30
  • Jul-20 Mo
O2k-Open SupportInfo: improve How can you compare respirometric experiments using an internal normalization?

Flux control ratios (FCR) are independent of external measurements (e.g. protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the ā€œO2 analysis template DL7.4ā€.
- Ā»Flux control ratioĀ«

Read more: Gnaiger Erich et al ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»Bioblast linkĀ« 


  • Jul-21 Tu
O2k-Publications in the MiPMap Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet
from the O2k-Network Ā»US TN Nashville Wasserman DHĀ« and Ā»US MN Minneapolis Hughey CĀ«
  • Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. Ā»Bioblast linkĀ«

  • Jul-22 We



  • Jul-23 Th
MitoPedia
First O2k-Webinar - O2k-Applications: H2O2 production by Dr. TĆ­mea KomlĆ³di

  • Jul-24 Fr
MitoPedia
Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations?

It is possible to do so using exogenously added cytochrome c:
- Ā»Cytochrome c control factorĀ«


2020 Week 31
  • Jul-27 Mo
O2k-Open SupportInfo: improve Do you know how to prepare a homogenate from tissue samples using the PBI-Shredder O2k-Set?

The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality.
- Ā»O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparationĀ«


  • Jul-28 Tu - World Hepatitis Day
O2k-brief repository Insights into Hepatitis C virus-linked mitochondrial dysfunction
from the O2k-Network Ā»IT Foggia Capitanio NĀ« 
  • Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1Ī±-mediated glycolytic adaptation. J Virol 84:647-60. Ā»Bioblast linkĀ«

  • Jul-29 We

  • Jul-30 Th
MitoPedia
Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:

- Ā»ChlororespirationĀ«


  • Jul-31 Fr


Jun 2020

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2020 Week 23 
  • Jun-01 Mo
O2k-Open SupportWarning: loss of data Did you know that instabilities of the slope of the oxygen signal (O2 slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O2 background test?

- Ā»Unstable 'O2 slope neg.'Ā« and  Ā»TIP2k Filter PapersĀ«


  • Jun-02 Tu
O2k-Publications in the MiPMap Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers
from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« , Ā»CA Rimouski Blier PUĀ« and Ā»CA Edmonton Lemieux HĀ«
  • Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. Ā»Bioblast linkĀ«

  • Jun-03 We

  • Jun-04 Th
MitoPedia: SUIT Mitochondria show variable preferences for glutamate & malate or pyruvate & malate to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.

DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates.
-Ā»SUIT-008Ā« and Ā»SUIT-014Ā«


  • Jun-05 Fr


2020 Week 24
  • Jun-08 Mo
MitoPedia
Celebrating World Oceans Day

Would you like to measure the O2 flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
- Ā»PhotoBiologyĀ«


  • Jun-09 Tu

  • Jun-10 We

  • Jun-11 Th
MitoPedia
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?

- Ā»Incompatible substratesĀ«



  • Jun-15 Th
O2k-Open SupportWarning: loss of data Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?

- Ā» Injection artefactĀ«


  • Jun-12 Fr
O2k-brief repository Oxygen consumption by intact platelets positively correlates with the concentration of CoQ10 in the platelets of CKD patients
from the O2k-Network Ā» SK Bratislava Sumbalova Z Ā« 
  • GvozdjĆ”kovĆ” A, SumbalovĆ” Z, KucharskĆ” J, KomlĆ³si M, RausovĆ” Z, VančovĆ” O, SzĆ”moÅ”ovĆ” M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. Ā»Bioblast linkĀ«
 2020 Week 25
  • Jun-15 Mo
O2k-Open SupportWarning: loss of data Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?

- Ā» Injection artefactĀ«


  • Jun-16 Tu
O2k-Publications in the MiPMap A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling
from the O2k-Network Ā»CA Montreal Hepple RTĀ«
  • Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. Ā»Bioblast linkĀ«

  • Jun-17 We

  • Jun-18 Th
MitoPedia
Did you know the Crabtree effect can affect respiration in living cells?

SUIT-003 is designed to analyze this important mechanism of respiratory control.
- Ā»SUIT-003 O2 ce D037Ā« and Ā»SUIT-003 O2 ce D038Ā«


  • Jun-19 Fr
2020 Week 26
  • Jun-22 Mo
O2k-Open SupportTake care Did you know how a stirrer test can save your experiments?

Get immediate information on how satisfactory your OroboPOS response time is by performing a stirrer test during air calibration.
- Ā»Oxygen calibration - DatLabĀ«


  • Jun-23 Tu
O2k-Publications in the MiPMap ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis
from the O2k-Network Ā»IT Verona Calabria EĀ«
  • Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an in vitro model of ALS. Front Neurosci 13:1070. Ā»Bioblast linkĀ«

  • Jun-24 We

  • Jun-25 Th
MitoPedia
Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?

- Ā» DithioniteĀ«


  • Jun-26 Fr
MitoGlobal
Join and disseminate the ā€œWomen in Mitochondriaā€ initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity.
 2020 Week 27
  • Jun-29 Mo
O2k-Open SupportWarning: loss of data Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?
An 'O2 slope neg.' within Ā± 2 pmolāˆ™sāˆ’1āˆ™mLāˆ’1 is optimal (Ā± 4 pmolāˆ™sāˆ’1āˆ™mLāˆ’1 is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.

- Ā» Air and zero calibrationĀ«


  • Jun-30 Tu
O2k-Publications in the MiPMap The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice
from the O2k-Network Ā»LV Riga Makrecka-Kuka MĀ«
  • Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. Ā»Bioblast linkĀ«


May 2020

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>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

2020 Week 19
  • May-04 Mo
O2k-Open SupportTake care Do you load the correct instrumental background correction according to the actual range of experimental O2 concentrations?

Perform and apply the instrumental background test:
- Ā» O2 background correction in DatLabĀ« and Ā» Instrumental oxygen backgroundĀ«


  • May-05 Tu
O2k-Publications in the MiPMap
Expression of alternative oxidase prevents reverse electron transfer
- from the O2k-Network Ā»FI Helsinki Jacobs HTĀ«  Ā»DE Magdeburg Gellerich FNĀ« Ā»FI Tampere Dufour EĀ« Ā»DE Magdeburg Debska-Vielhaber GĀ« Ā»DE Frankfurt Wittig IĀ« and Ā»UK Brighton Moore ALĀ«
  • Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. Ā»Bioblast linkĀ«

  • May-06 We

  • May-07 Th
MitoPedia: SUIT Quantify fatty acid oxidation capacity (FAO, F-pathway), avoiding a common artefact of overestimation. Malate anaplerotic pathways (see mt-malic enzyme) are active in various tissues and cultures cells, which requires caution: Ā»Malate-anaplerotic pathway control stateĀ«

- DatLab 7.4 provides a step-by-step guide to evaluate the malate anaplerotic pathway - Ā»SUIT-027Ā«

- Since F-pathway capacity can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - Ā»SUIT-002Ā«



  • May-08 Fr
2020 Week 20
  • May-11 Mo
O2k-Open SupportTake care Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.

Learn more about steady states including performance and mark setting for data analysis
- Ā»Steady stateĀ«


  • May-12 Tu
O2k-Publications in the MiPMap Mitochondria - a driving force in adipocyte differentiation
from the O2k-Network Ā»CZ Pilsen Kuncova JĀ« 
  • KladnickĆ” I, ČedĆ­kovĆ” M, KripnerovĆ” M, DvoÅ™Ć”kovĆ” J, KohoutovĆ” M, TÅÆma Z, MĆ¼llerovĆ” D, KuncovĆ” J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. Ā»Bioblast linkĀ«

  • May-13 We

  • May-14 Th
MitoPedia
How can I measure ATP production with the O2k?

Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- Ā»Magnesium GreenĀ«


  • May-15 Fr
O2k-brief repository Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse
from the O2k-Network Ā»FI Helsinki Jacobs HTĀ« , Ā»DE Magdeburg Gellerich FNĀ« , Ā»FI Tampere Dufour EĀ« , Ā»DE Magdeburg Debska-Vielhaber GĀ« and Ā»DE Frankfurt Wittig IĀ«
  • Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. Ā»Bioblast linkĀ«

  • May-17 Su World Hypertension Day
O2k-Publications in the MiPMap Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats
from the O2k-Network Ā»CZ Prague Houstek JĀ« and Ā»CZ Prague Kalous MĀ«
  • Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. Ā»Bioblast linkĀ«
2020 Week 21 
  • May-18 Mo
O2k-Open SupportWarning: loss of data Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?

See how to clean and dry the electrical connections of the OroboPOS-Connector.
- Ā»Cleaning the electrical connectionsĀ«


  • May-19 Tu
O2k-Publications in the MiPMap Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cellsĀ«
from the O2k-Network Ā»Charles Anne-LaureĀ« Ā»Geny BernardĀ«
  • Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard
    (2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. Ā»Bioblast linkĀ«

  • May-20 We
SI Units SI units

One year ago, on World Metrology Day (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of Mitochondrial physiology and with the launch of an innovative journal ā€” Bioenergetics Communications.

Bioenergetics Communications MitoEAGLE Consortium Communication: Mitochondrial physiology ā€• Published online: 2020-05-20.

Gnaiger E et al ā€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1
Ā» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1


  • May-21 Th
MitoPedia
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O2 consumption and production.

-Ā»PhotoBiologyĀ«


  • May-22 Fr
2020 Week 22 
  • May-25 Mo
O2k-Open SupportTake care Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?

Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.
- Ā»Switch components approachĀ«


  • May-26 Tu
O2k-brief repository Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes
from the O2k-Network Ā» AT Innsbruck Gnaiger E Ā« and Ā» AT Innsbruck Oroboros Ā« 
  • Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Santā€™Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. Ā»Bioblast linkĀ«

  • May-27 We
Gentle Science
The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine

To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus


  • May-28 Th
MitoPedia
Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas?

- Ā»Nitrogen injectionĀ«


  • May-29 Fr
O2k-Publications in the MiPMap F1FO-ATP synthase regulates alternative oxidase in Trypanosoma brucei
from the O2k-Network Ā»BR Sao Paulo Silber AMĀ«
  • Alberto LuĆ©vano-MartĆ­nez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. Ā»Bioblast linkĀ«


Apr 2020

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2020 Week 14
  • Apr-01 We
O2k-Publications in the MiPMap Parkinsonā€™s Disease Awareness Month: Dominant mutation in Parkinsonā€™s Disease causes loss of protein function in mitochondria
- from the O2k-Network Ā»FR Angers Gueguen NĀ« and Ā»BE Leuven Spinazzi MĀ«
  • Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. Ā»Bioblast linkĀ«

Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry
  • Apr-02 Th
MitoPedia: SUITDo you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: Ā»SUITbrowserĀ«



O2k-Network Creative solutions coming from the O2k-Network labs - Ā» NZ Auckland Hickey AJ




  • Apr-03 Fr
O2k-brief repository Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation
- from the O2k-Network Ā»DE Nuthetal Klaus SĀ«
  • Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - Ā»Bioblast linkĀ«


 2020 Week 15
  • Apr-06 Mo
The world of humankind is and will be different during and after the COVID-19 crisis ā€“ a fact beyond our competence. How different the world will be ā€“ depends on our ability to learn and communicate.
O2k-Publications in the MiPMap Physical activity has positive effects on mitochondria from patients with occupational burnout
- from the O2k-Network Ā»CH Basel Eckert AĀ«
  • Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - Ā»Bioblast linkĀ«



  • Apr-07 Tu
DatLab Info: improve Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4?'
If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - Ā»SUIT: Browse DL-Protocols and templatesĀ«

  • Apr-08 We
MitoPedia: SUITThe optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - Ā»SUIT-010Ā«

  • Apr-09 Th

  • Apr-10 Fr
 2020 Week 16
  • Apr-13 Mo
MitoPedia: SUIT You can quantify the ATPase activity in your mitochondrial preparation:

- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of mitochondrial preparations - Ā»SUIT-024Ā«



  • Apr-14 Tu
O2k-Open SupportTake care Why are the oxygen levels different between chambers prior to my experimental run?
After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - Ā» Oxygen calibration - troubleshootingĀ«

  • Apr-15 We

  • Apr-16 Th
O2k-brief repository Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease
- from the O2k-Network Ā»DE Frankfurt Eckert GPĀ« Ā»CH Basel Eckert AĀ«
  • Leuner K, Schulz K, SchĆ¼tt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, MĆ¼ller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. - Ā»Bioblast linkĀ«



  • Apr-17 Fr
2020 Week 17
  • Apr-20 Mo
O2k-brief repository Mitochondrial respiration medium MiR05 wins - KCl inhibits
- from the O2k-Network Ā»US TN Nashville Wasserman DHĀ« and Ā»US MI East Lansing Bazil JNĀ«
  • Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - Ā»Bioblast linkĀ«


O2k-Open SupportInfo: improve Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - Ā» Pyruvate - troubleshootingĀ«

  • Apr-21 Tu
Citrate synthase
MitoPedia Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- Ā»MiPNet17.04 CitrateSynthaseĀ«



  • Apr-22 We

  • Apr-23 Th

  • Apr-24 Fr
Electron transport chain
MitoPedia ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- Ā»Hatefi 1962 J Biol Chem-XLIIĀ«


 2020 Week 18
  • Apr-27 Mo
O2k-Open Support
Take care Do you set marks correctly in the instrumental O2 background test when planning to apply the background correction to a SUIT protocol with a high O2 regime?
The range of O2 concentrations selected in the instrumental background test should correspond to the range of experimental O2 concentrations. - Ā» O2 Background correction - troubleshootingĀ«



  • Apr-28 Tu
O2k-Publications in the MiPMap
Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake
- from the O2k-Network Ā»FI Helsinki Jacobs HTĀ«, Ā»DE Magdeburg Gellerich FNĀ« and Ā»DE Magdeburg Debska-Vielhaber GĀ«
  • Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, LeƟmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - Ā»Bioblast linkĀ«

  • Apr-29 We

  • Apr-30 Th



Mar 2020

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2020 Week 10
  • Mar-02 Mo
O2k-Network
An amazing number of new O2k-Publications from China. - Ā»O2k-Network Labs ChinaĀ«



O2k-Publications in the MiPMap Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction
- from the O2k-Network Ā»CN Chongqing Zhu ZĀ«
  • Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - Ā»Bioblast linkĀ«



  • Mar-03 Tu
O2k-Publications in the MiPMap Rapamycin suppresses senescence in galactose versus glucose cell culture
- from the O2k-Network Ā»US PA Philadelphia Orynbayeva ZĀ«
  • Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - Ā»Bioblast linkĀ«


MitoPedia Rapamycin - more differences or more in common with other mitObesity drugs? - Ā»RapamycinĀ«


Gentle Science
Advancing Open Science practices: stakeholder perspectives on incentives and disincentives. National Academies of Sciences, Engineering, and Medicine (2020) - Ā»Bioblast linkĀ« - The concept of Preprints needs full attention in Open Science.



  • Mar-04 We
Platelet.jpg
O2k-Publications in the MiPMap Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets
- from the O2k-Network Ā»US FL Orlando Goodpaster BHĀ«
  • Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - Ā»Bioblast linkĀ«
Softdrink.jpg
O2k-brief repository Sugar and fat - initial mitochondrial aging in the brain
- from the O2k-Network Ā»IT Naples Iossa SĀ«
  • Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - Ā»Bioblast linkĀ«
OroboPOS-Holder002.jpg


O2k-Open Support Warning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact. - Ā»O2k-OroboPOS-HolderĀ«




  • Mar-05 Th


MitoFit
With a preprint you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - Ā»PreprintĀ«



  • Mar-06 Fr
MitoPedia: SUIT Explore metabolic profiles and specific modultations of mitochondrial pathways in health and disease with SUIT reference protocols. - Ā»RP1Ā« and Ā»RP2Ā«

Gentle Science
Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors. National Academies of Sciences, Engineering, and Medicine (2020) - Ā»Bioblast linkĀ«


 2020 Week 11
  • Mar-09 Mo
COST Action MitoEAGLE Ā»MitoEAGLE WG2 Innsbruck ATĀ« (Mar 09-13)


O2k-Publications in the MiPMap Protein-specific mitochondrial respiratory capacity is higher in women than men
- from the O2k-Network Ā»SE Stockholm Cardinale DAĀ«
  • Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - Ā»Bioblast linkĀ«



  • Mar-10 Tu
O2k-Publications in the MiPMap Muscular exercise for training adipose tissue mitochondria
- from the O2k-Network Ā»DK Copenhagen Larsen SĀ«
  • Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - Ā»Bioblast linkĀ«

  • Mar-11 We
MitoPedia Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - Ā»BlebbistatinĀ«


Gentle Science
Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science


O2k-Open Support Info: improve Do you change the slope smoothing to 20 while operating the O2k with biological sample?
- It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before uncoupler titrations. - Ā»Slope smoothingĀ«

  • Mar-12 Th
O2k-Publications in the MiPMap Multiple Sclerosis Month: Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis
- from the O2k-Network Ā»UK London Duchen MRĀ«
  • Warne J, Pryce G, Hill J, Shi X, LennerĆ„s F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - Ā»Bioblast linkĀ«

  • Mar-13 Fr
O2k-Publications in the MiPMap Men and women with Type 1 diabetes and skeletal muscle mitochondria
- from the O2k-Network Ā»CA Toronto Perry CGĀ«
  • Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - Ā»Bioblast linkĀ«
 2020 Week 12  
  • Mar-16 Mo
Oroboros
Oroboros operates from now on in home office mode - we stay connected.


MitoPedia: SUIT
The standardized coupling control protocol to explore the bioenergetics of living cells
- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - Ā»SUIT-003Ā«



  • Mar-17 Tu
O2k-Publications in the MiPMap
Zika virus infection leads to mitochondrial dysfunction
- from the O2k-Network Ā»BR Rio de Janeiro Galina AĀ«
  • Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - Ā»Bioblast linkĀ«

  • Mar-18 We
O2k-brief repository Exercise protects against the effects of aging on mitochondrial function
- from the O2k-Network Ā»KR Busan Han JĀ« Ā»KR Incheon Kwak HBĀ«
  • No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - Ā»Bioblast linkĀ«



  • Mar-19 Th
DatLab
Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home.
- Ā» For the download link and password, please email instruments@oroboros.at



  • Mar-20 Fr
 2020 Week 13
  • Mar-23 Mo
O2k-Open Support Take care Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background test when using the O2k-sV-Module?
(1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background test can be performed with the "BG Feedback" settings. - Ā»DithioniteĀ« 
(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O2 background test can be performed using the O2k-sV-Module. - Ā»O2k-sV-Module ManualĀ«



  • Mar-24 Tu
O2k-Publications in the MiPMap Tuberculosis Day: Mitochondria as target to Mycobacterium tuberculosis infection
- from the O2k-Network Ā»US NC Durham Li PAĀ«
  • Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - Ā»Bioblast linkĀ«

  • Mar-25 We
MitoFit
Preprint: An urgent call for research on COVID-19 and public vitamin D awareness
  • Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? ā€“ An urgent call for research. MitoFit Preprint Arch doi:10.26124/mitofit:200001.


Gentle Science
Research findings made available via preprint servers before journal publication - Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak


O2k-Open Support Info: improveO2 flux analysis with DatLab 7.4. Use our latest O2 analysis template DL7.4: Ā»O2k-Videosupport: O2 flux analysis with DatLab 7.4Ā«

  • Mar-26 Th

  • Mar-27 Fr
2020 Week 14
  • Mar-30 Mo
O2k-Open Support Info: improve DatLab 7.4 offers the flexibility to edit a DL-Protocol: Ā»Enable DL-Protocol editingĀ«

  • Mar-31 Tu


Feb 2020

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 2020 Week 6
  • Feb-03 Mo
FFG Ā»Official start of the FFG funded project Ā»MitoKINĀ«
O2k-Publications in the MiPMap Blood cell mitochondrial function does not provide a general substitute for muscle biopsies
- from the O2k-Network Ā»US AR Little Rock Boersheim EĀ«
  • Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - Ā»Bioblast linkĀ«
  • Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, Dr Nelly Tremblay is using for the first time the SUIT-022 protocol to study alternative oxidases in the marine model organism Phaeodactylum tricornutum.



  • Feb-04 Tu
O2k-Workshops MiPNet25.10 IOC and Distributor engagement Tokyo JP (Feb 4-7)
O2k-brief Cronin (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - Lymphocytes, Cancer - O2k-brief repository



  • Feb-05 We
O2k-Publications in the MiPMap Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimerā€™s disease
- from the O2k-Network Ā»US FL Boca Raton Khamoui AVĀ«
  • Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - Ā»Bioblast linkĀ«



  • Feb-06 Th
MitoFit Open Seminar
  • 16:00-16:30 Jorge Beleza: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. Ā»AbstractĀ«
  • 16:30-17:00 Rita Pereira: The Handball4Health Project : A community-based intervention programme
 2020 Week 7
  • Feb-10 Mo

  • Feb-12 We
MitoPedia What do metformin, melatonin and other bioactive mitObesity compounds have in common? - Ā»MitObesity drugsĀ«


O2k-Feedback
I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner. - Gayathri Narayanappa participaing in MiPNet25.03 IOC144 Innsbruck AT



  • Feb-13 Th
2020 Week 8
  • Feb-17 Mo
EBEClogo2020.jpg Registration is open: European Bioenergetics Conference 2020, Marseille, France, 2020

  • Feb-18 Tu
MitoPedia Why do we fight gas bubbles? Ā» Dive deep into Oxygen solubility
O2k-Publications in the MiPMap Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis
- from the O2k-Network Ā»US MI Ann Arbor Beard DAĀ«
  • Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298ā€“314. - Ā»Bioblast linkĀ«



  • Feb-19 We
MitoFit Preprints.png - Ā»MitoFit Preprint ArchĀ« was initiated one year ago. We invite submissions of mitochondrial preprints.
MitoPedia Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - Ā»SpermidineĀ«



  • Feb-20 Th
Logo MitoFit s.jpg - MitoFit Open Seminar: Eduard Stefan: ā€œDecoding and Perturbation of ONCO-Kinase functionsā€ and Omar Torres Quesada: ā€œMitoKIN - Systematic barcoding of kinase-controlled mitochondrial functionā€


2020 Week 9
  • Feb-23 Su
Ā» 2020 PaduaMuscleDays Padova IT - postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy

  • Feb-24 Mo
MitoPedia
More research is needed on the impact of viral infection on mitochondrial function. - Ā»Viruses and mitochondrial medicineĀ«



  • Feb-25 Tu
O2k-Network
High quality mitochondrial research is increasing rapidly in South Korea. - Ā»O2k-Network Labs Republic of KoreaĀ«



O2k-Publications in the MiPMap An active lifestyle protects cardiac mitochondria
- from the O2k-Network: KR Busan Han J, KR Incheon Kwak HB
  • No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - Ā»Bioblast linkĀ«



MitoEAGLE WG4 retreat; left to right: Luiz, Erich, Eleonor, Slawomir
  • Feb-26 We
MitoPedia
It is essential to titrate uncouplers in multiple steps for respirometric measurement of ET capacity. - Ā»Noncoupled respirationĀ«


COST Action MitoEAGLE MitoEAGLE WG4 Innsbruck AT (Feb 26-28)

  • Feb-27 Th
O2k-Publications in the MiPMap Regulation of mitochondrial oxygen kinetics by cytochrome c oxidase
- from the O2k-Network: CZ Prague Houstek J
  • Pajuelo-Reguera David, ČunĆ”tovĆ” KristĆ½na, VrbackĆ½ Marek, PecinovĆ” Alena, HouÅ”těk Josef, MrĆ”Äek TomĆ”Å”, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - Ā»Bioblast linkĀ«

  • Feb-28 Fr
O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Ā» Oxygen calibration - DatLabĀ«


Jan 2020

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 2020 Week 1 The Oroboros team enjoys holidays from 2019 Dec 24 to 2020 Jan 06
Oroboros
Oroboros picture of the month

Oroboros of the month

Season's Greetings
Our office is closed until 2020-01-06.
We wish you a Happy New Year 2020!
Your Oroboros team



  • Jan-04 Sa
News The Oroboros Ecosystem Agenda 2020 is launched as a signature of the Oroboros Ecosystem.
Gentle Science 2020: a critical year for women, gender equity, and health - a Lancet Editorial
  • One month ago
Oroboros
NextGen-O2k SME2 coaching Orily Pratt part 2

MitoFit Guest Seminar
On December 03/04 the second part of the SME phase 2 Coaching for the NextGen-O2k project with Orily Pratt (Tel Aviv, IL) took place in Innsbruck.



Ā» Wednesday, 4th of December; Oroboros Instruments, Schoepfstrasse 18, MiPArt, Innsbruck, Austria.
 2020 Week 2
  • Jan-07 Tu The Oroboros Office opens in the New Year 2020
EBEClogo2022.jpg
  • From the Austrian Bioenergetics Group to Mitochondrial Physiology and Bioenergetics DACH (D: Germany; A: Austria; CH: Switzerland)
EBEC22 - the 22nd European Bioenergetics Conference will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the EBEC22 National Scientific Committee. This provides an excellent opportunity to establish an Austrian Bioenergetics Group towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming 1st Myocardial Function Symposium 2020 Graz AT, and further at the MitoEAGLE Summit Obergurgl 2020. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at



  • Jan-08 We
MitoPedia Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.
Quality



  • Jan-09 Th
COST Action MitoEAGLE
Members of the Management Committee MitoEAGLE and Working Group Leaders: please submit your suggestions for MitoEAGLE Summit Obergurgl 2020:
  1. Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output
  2. Suggested scientists (titles) for presentations, according to the MoU



  • Jan-10 Fr
Ā» 1st Myocardial Function Symposium 2020 Graz AT (Jan 10-11)
  • 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)



  • Jan-12 So
  • One month ago
Oroboros
Cluster Awards 2019

Cluster Awards 2019

On December 12th, the Cluster Awards 2019 were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the Life Sciences Cluster for our EU Horizon 2020-funded NextGen-O2k project.

Life Sciences

Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"

Renewable Energies

Heliotherm WƤrmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"

Forest and wood

Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"

Information Technologies

Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"

Mechatronics: ALPEX Technologies GmbH, Mils: "EVEREST 4.0"

Wellness: Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"

Ā» More details (in German): Standortagentur Tirol.

Photo Ā© Standortagentur Tirol
 2020 Week 3
  • Jan-14 Tu



  • Jan-15 We
O2k-Publications in the MiPMap Statistical evaluation on replica in HRR with permeabilized skeletal muscle fibers
- from the O2k-Network Ā»AU Melbourne Stepto NKĀ«
  • Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - Ā»Bioblast linkĀ«



  • Jan-16 Th
MitoPedia Oxygen flux - which SI units should be used?
MitoPedia Did you ever think that units for body mass could be an issue? - Ā»Body massĀ«



  • Jan-17 Fr
About Oroboros HRR - high-resolution respirometry and oxygen kinetics 10 years ago: Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010. - Ā»Bioblast linkĀ«
TRANSMIT TRANSMIT Symposium 2020 Brussels BE (Jan 17-18)


O2k-Publications in the MiPMap Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations
- from the O2k-Network Ā»CZ Prague Krajcova AĀ«
  • KrajčovĆ” A, Urban T, Megvinet D, Waldauf P, BalĆ­k M, Hlavička J, Budera P, JanouÅ”ek L, PokornĆ” E, DuÅ”ka F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - Ā»Bioblast linkĀ«
2020 Week 4
  • Jan-20 Mo
O2k-Workshops IOC144 Innsbruck AT - O2k-Coaching Days (Jan 20-22)



  • Jan-21 Tu
  • Mr Hai Wu (CEO of Huawei, Oroboros distributor in China) and Dr. Rupert Ecker (CEO of Vienna TissueGnostics) join the O2k-Coaching Days for extending collaborative projects.



  • Jan-23 Th - MitoFit Open Seminar - Erich Gnaiger: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.



  • Jan-24 Fr
O2k-Open Support Info: improve Do you write the serial number of the polarographic oxygen sensor (POS) in the ā€˜O2k configurationā€™ window?
Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - Ā»O2k configurationĀ«
O2k-Open Support Warning: loss of data Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - Ā»O2k-chamberĀ«
O2k-Open Support Take care Do you use the correct O2 solubility factor (FM) in the ā€˜O2 calibrationā€™ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration. - Ā»Oxygen solubility factorĀ«

2020 Week 5

  • Jan-27 Mo
O2k-Workshops IOC145 Innsbruck AT - O2k-Coaching Days (Jan 27-29)
DSL-Seminar, 16:00
Gnaiger E: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.
Univ.-Klinik fĆ¼r Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room



  • Jan-28 Tu
O2k-Publications in the MiPMap Mitochondrial respiration of blood cells and cardiovascular disease
- from the O2k-Network Ā»FR Strasbourg Zoll JĀ«
  • Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - Ā»Bioblast linkĀ«



  • Jan-30 Th - MitoFit Open Seminar - Alexander Karabatsiakis: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.
O2k-Open Support Take care Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - Ā»Oxygen calibration - DatLabĀ«
O2k-Open Support Info: improve Do you set mark ā€˜R1ā€™ and complete the air calibration correctly?
Your air calibration is not effective until you set mark ā€˜R1ā€™ andclick on Calibrate and copy to clipboard in the O2 calibration window. - Ā»Oxygen calibration - DatLabĀ«




  • Jan-31 Fr
O2k-Open SupportTake care Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - Ā»Oxygen calibration - DatLabĀ«


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