Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Oroboros Laboratory"

From Bioblast
Line 2: Line 2:
[[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]]
[[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]]
[[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
[[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
__TOC__
__TOC__
== About us ==


The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas. Β 
Β 
:::: The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas. Β 


=== O2k instruments ===
=== O2k instruments ===
[[File:OROBOROS MitoFit Lab IMG 7638.JPG|right|500px |Lisi & Yvonne, MitoFit Laboratory]]
[[File:OROBOROS MitoFit Lab IMG 7638.JPG|right|500px |Lisi & Yvonne, MitoFit Laboratory]]
'''28 O2k-Chambers (14 O2k)'''
:::: '''28 O2k-Chambers (14 O2k)'''


* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
::::* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.


* Two additional O2ks (4 chambers) are devoted to instrumental development.
:::: * Two additional O2ks (4 chambers) are devoted to instrumental development.




''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]]
::::Β» ''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]]




=== OXPHOS analysis ===
=== OXPHOS analysis ===


SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
:::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
Β 
* a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;


* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).
::::* coupling control protocols in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).




=== The MitoFit Centre of Excellence ===
=== The MitoFit Centre of Excellence ===


OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
:::: OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.


According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
:::: According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.


[[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]]
[[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]]


::::Β» [[OROBOROS_Company#Current_projects |'''Current projects and collaborators''']]


Β 
::::Β» [[OROBOROS_MitoFit_lab:_visiting_scientists |'''Visiting scientists''']]
== Current projects and collaborators ==
:: '''The [[OROBOROS_Company#The_OROBOROS-Team |OROBOROS-Team]] collaborates with:'''
Β 
=== Pharmacology: mitochondrial function in brain tissues ===
[[Image:Johannes.jpg|left|90px|Johannes Burtscher]]
:: '''[[Burtscher J|Johannes Burtscher]]''', Mag. PhD
:: FWF projects W1206-B05 and I-977-B24.
:: Project leader: A.Univ. Prof. Dr. Christian Schwarzer, Dept. Pharmacology, Medical University Innsbruck, Austria
<br />
Β 
=== Internal Medicine ===
[[Image:VolaniC.JPG|left|90px|Chiara Volani]]
:::: '''[[Volani C| Chiara Volani]]''', [mailto:c_volani@yahoo.com c_volani@yahoo.com]
:::: Project leader: Prof. Dr. GΓΌnther Weiss, Dept. of Internal Medicine, Medical University Innsbruck, Austria
<br />
Β 
Β 
=== MitoFit project ===
Β 
:::: '''[[Hoppel F|Florian Hoppel]]''', MSc.
:::: PhD student (discontinued)
:::: Institute for Sports Science and OROBOROS: [[K-Regio MitoFit]]
[[Image:MitoFit.jpg|100px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]
<br/><br/>
Β 
Β 
=== Oncotyrol ===
:::: '''Prostate cancer'''
:::: The [[Oncotyrol| Oncotyrol]] research project has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue, and comparison of primary tissue with cultured cells. - [http://oroboros.at/index.php?id=oncotyrol Oncotyrol]
:::: Project leader: A.Univ. Prof. Mag. Dr. Helmut Klocker, Dept. Urology, Medical University Innsbruck, Austria
Β 
::::'''[[Weber A| Anja Weber]]''', Mag., PhD student - [mailto:anja.weber@i-med.ac.atΒ  anja.weber@i-med.ac.at]
Β 
[[Image:SchoepfB.jpg|left|90px]]
:: '''[[Schoepf B|Bernd Schoepf]]''', Mag., PhD student -
<br/>
<br/>
<br/>
Β 
Β 
=== Visiting scientists ===
:::: [[OROBOROS_MitoFit_lab:_visiting_scientists |'''Β» Visiting scientists in the MitoFit lab''']]

Revision as of 17:37, 3 November 2016

                



Oroboros Laboratory

MitoFit Laboratory
MitoFit


The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, June 2013. It comprises 140 sqm which are divided into the office and research areas.

O2k instruments

Lisi & Yvonne, MitoFit Laboratory
28 O2k-Chambers (14 O2k)
  • Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
* Two additional O2ks (4 chambers) are devoted to instrumental development.


Β» More details: OROBOROS Support


OXPHOS analysis

SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
  • coupling control protocols in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
  • various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).


The MitoFit Centre of Excellence

OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.

ORO Lab 3.jpg ORO Lab 4.jpg ORO Lab 2.jpg

Β» Current projects and collaborators
Β» Visiting scientists