Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Oroboros Laboratory"

From Bioblast
(46 intermediate revisions by 9 users not shown)
Line 1: Line 1:
{{Template:OROBOROS header page name}}
{{Template:OROBOROS header page name}}
[[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]]
{{Template:OROBOROS Slogan}}
[[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
__TOC__
== About us ==


The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], starting in June 2013. It comprises 140 sqm which are divided into the office and research areas.
:::: <big>'''Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab'''</big>


=== O2k instruments ===
:::: <big>'''Scientists with a gentle touch of entrepreneurship'''</big>
'''24 O2k-Chambers (twelve O2k)'''
[[File:Lab in action.jpg|right|500px|Oroboros Laboratory]]


* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.


* Two additional O2ks are devoted to instrumental development.


<!--[[File:OROBOROS MitoFit-Lab.jpg|left|240px |Oroboros O2k-Laboratory]]-->


''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]]
__TOC__
 
 
=== OXPHOS analysis ===
 
SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
 
* a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
 
* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).


== Oroboros O2k-Laboratory ==
:::: '''48 O2k-Chambers (24 O2k)'''


=== The MitoFit Centre of Excellence ===
:::: The '''Oroboros O2k-Laboratory''' ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]] in June 2013. It comprises 140 sqm which are divided into the office and research areas.


OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
::::* Twenty four O2k instruments for HRR (48 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.


According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
::::* Two additional O2ks (4 chambers) are devoted to instrumental development.


[[File:ORO Lab 1.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] [[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]]
::::* According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world.


::::» ''More details:'' [[O2k-technical_support_and_open_innovation|Oroboros Support]]




== Current projects and collaborators ==
=== Collaborators in the Oroboros Ecosystem ===
:: '''The [[OROBOROS_Company#The_OROBOROS-Team |OROBOROS-Team]] collaborates with:'''
{| style="color: black;  width: 90%"
|-
| style="text-align: center;  background-color: #EFF5FB" | '''Collaborations'''
| style="text-align: center;  background-color: #ffffff" |
<gallery heights="165px" mode="nolines" perrow="6" widths="192px">
Image:LiebscherG.JPG|'''[[Liebscher G|Gudrun Liebscher]]''', MSc., PhD. student
Image:LemanG.JPG|'''[[Leman G|Géraldine Leman]]''', Postdoctoral researcher
File:Solmaz.png|'''[[Etemad S|Solmaz Etemad]]''', Postdoctoral researcher
Image:FischerC.JPG|'''[[Fischer C| Christine Fischer]]''', MSc., PhD. student
File:Lisa Bergmeister.jpg|'''[[Bergmeister L|Lisa Bergmeister]]''', PhD. student - [[SPIN]]
</gallery>
|}


=== MitoFit project ===


[[Image:HoppelF.jpg|left|90px]]


:::: '''[[Hoppel F|Florian Hoppel]]''', MSc. - [mailto:florian.hoppel@oroboros.at florian.hoppel@oroboros.at]
:::: PhD student
:::: Institute for Sports Science and OROBOROS: [[O2k-MitoFit]]
[[Image:O2k-MitoFit.jpg|100px|link=http://www.mitofit.org/index.php/O2k-MitoFit|O2k-MitoFit]]
<br/><br/>


== The DSL-Oroboros Research Laboratory ==
:::: '''16 O2k-Chambers (8 O2k)'''
:::: {{Template:Oroboros Ecosystem Partner}}


=== Oncotyrol ===
:::: The '''DSL-Oroboros Research Laboratory''' at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells.
:::: '''Prostate cancer'''
:::: '''Oroboros Instruments''' collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.
:::: The [[Oncotyrol| Oncotyrol]] research project has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue, and comparison of primary tissue with cultured cells. - [http://oroboros.at/index.php?id=oncotyrol Oncotyrol]
:::: Project leader: A.Univ. Prof. Mag. Dr. Helmut Klocker, Dept. Urology, Medical University Innsbruck, Austria




[[Image:WeberB.JPG|left|90px]]
=== Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory ===
::::'''[[Weber A| Anja Weber]]''', Mag., PhD student - [mailto:anja.weber@i-med.ac.at  anja.weber@i-med.ac.at]


[[Image:SchoepfB.jpg|left|90px]]
{| style="color: black;  width: 90%"
:: '''[[Schoepf B|Bernd Schoepf]]''', Mag., PhD student - [mailto:bernd.schoepf@student.i-med.ac.at bernd.schoepf@student.i-med.ac.at]
|-
<br/>
| style="text-align: center;  background-color: #ffffff" | '''[[Oroboros Laboratories |DSL-Oroboros Research Laboratory]]'''  
<br/>
<br/>


'''Medical University of Innsbruck'''
| style="text-align: center;  background-color: #EFF5FB" | <gallery heights="165px" mode="nolines" perrow="6" widths="192px">
Image:MeszarosA.JPG|'''[[Meszaros A|András Mészáros]]''', MD., PhD.<br/> - Mitochondrial Medicine Visionary
Image:David.jpg|'''[[Harrison DK| David Harrison]]''', Doz., PhD. - Independent scientific consultant
Image:MittelbergerC.JPG|'''[[Mittelberger C| Christa Mittelberger]]'''<br/> Student
Image:UnterwegerJ.JPG|'''[[Unterweger J| Jasmin Unterweger]]'''<br/> Student
Image:ReyerB.JPG|'''[[Reyer BA| Beatrix Ann Reyer]]'''<br/> Student
Image:HofmannJ.jpg|'''[[Hofmann J| Julia Hofmann]]'''<br/> Student
Image:Profile-icon-9.png|'''[[Buch M| Madita Buch]]'''<br/> Student
</gallery>


:::: '''Oncotyrol phasing out'''
:::: Project leader: Univ.-Prof. Dr. Zlatko Trajanoski, Biozentrum Innsbruck - Sektion für Bioinformatik, Medical University Innsbruck, Austria


::::'''[[Weber B| Benno Weber]]''', Mag., Scientific assistant - [mailto:benno.weber@oncotyrol.at benno.weber@oncotyrol.at]
|}
::::'''[[Laschober G| Gerhard Laschober]]''', Mag., PhD, Postdoc - [mailto:gerhard.laschober@i-med.ac.at gerhard.laschober@i-med.ac.at]


== OXPHOS analysis ==


=== Pharmacology: mitochondrial function in brain tissues ===
:::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
[[Image:Johannes.jpg|left|90px|Johannes Burtscher]]
:: '''[[Burtscher J|Johannes Burtscher]]''', Mag., PhD student
:: FWF projects W1206-B05 and I-977-B24.
:: Project leader: A.Univ. Prof. Dr. Christian Schwarzer, Dept. Pharmacology, Medical University Innsbruck, Austria
<br />


::::* coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).


=== Internal Medicine ===
:::: '''[[Volani C| Chiara Volani]]''', [mailto:c_volani@yahoo.com c_volani@yahoo.com]
:::: Project leader: Prof. Dr. Günther Weiss, Dept. of Internal Medicine, Medical University Innsbruck, Austria


=== Visiting scientists ===
::::» [[Projects|'''Current projects''']]
:::: [[OROBOROS_MitoFit_lab:_visiting_scientists |'''» Visiting scientists in the MitoFit lab''']]
::::» '''[[Partners in the Oroboros Ecosystem]]'''
::::» [[Oroboros Laboratories: visiting scientists |'''Visiting scientists''']]

Revision as of 14:04, 17 February 2020

                



Oroboros Laboratory

Mitochondria and Cell Research - the Oroboros Ecosystem
Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab
Scientists with a gentle touch of entrepreneurship
Oroboros Laboratory



Oroboros O2k-Laboratory

48 O2k-Chambers (24 O2k)
The Oroboros O2k-Laboratory ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project MitoCom Tyrol in June 2013. It comprises 140 sqm which are divided into the office and research areas.
  • Twenty four O2k instruments for HRR (48 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
  • Two additional O2ks (4 chambers) are devoted to instrumental development.
  • According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world.
» More details: Oroboros Support


Collaborators in the Oroboros Ecosystem

Collaborations



The DSL-Oroboros Research Laboratory

16 O2k-Chambers (8 O2k)
- a partner in the Oroboros Ecosystem
The DSL-Oroboros Research Laboratory at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells.
Oroboros Instruments collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.


Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory

DSL-Oroboros Research Laboratory

Medical University of Innsbruck


OXPHOS analysis

SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
  • coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
  • various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).


» Current projects
» Partners in the Oroboros Ecosystem
» Visiting scientists