Difference between revisions of "Oroboros Laboratory"
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{{Template:OROBOROS header page name}} | {{Template:OROBOROS header page name}} | ||
{{Template:OROBOROS Slogan}} | |||
:::: <big>'''Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab'''</big> | |||
:::: <big>'''Scientists with a gentle touch of entrepreneurship'''</big> | |||
''' | [[File:Lab in action.jpg|right|500px|Oroboros Laboratory]] | ||
<!--[[File:OROBOROS MitoFit-Lab.jpg|left|240px |Oroboros O2k-Laboratory]]--> | |||
__TOC__ | |||
== Oroboros O2k-Laboratory == | |||
:::: '''48 O2k-Chambers (24 O2k)''' | |||
:::: The '''Oroboros O2k-Laboratory''' ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]] in June 2013. It comprises 140 sqm which are divided into the office and research areas. | |||
::::* Twenty four O2k instruments for HRR (48 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations. | |||
* | ::::* Two additional O2ks (4 chambers) are devoted to instrumental development. | ||
* | ::::* According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world. | ||
::::» ''More details:'' [[O2k-technical_support_and_open_innovation|Oroboros Support]] | |||
=== Collaborators in the Oroboros Ecosystem === | |||
{| style="color: black; width: 90%" | |||
|- | |||
| style="text-align: center; background-color: #EFF5FB" | '''Collaborations''' | |||
| style="text-align: center; background-color: #ffffff" | | |||
<gallery heights="165px" mode="nolines" perrow="6" widths="192px"> | |||
Image:LiebscherG.JPG|'''[[Liebscher G|Gudrun Liebscher]]''', MSc., PhD. student | |||
Image:LemanG.JPG|'''[[Leman G|Géraldine Leman]]''', Postdoctoral researcher | |||
File:Solmaz.png|'''[[Etemad S|Solmaz Etemad]]''', Postdoctoral researcher | |||
Image:FischerC.JPG|'''[[Fischer C| Christine Fischer]]''', MSc., PhD. student | |||
File:Lisa Bergmeister.jpg|'''[[Bergmeister L|Lisa Bergmeister]]''', PhD. student - [[SPIN]] | |||
</gallery> | |||
|} | |||
== The DSL-Oroboros Research Laboratory == | |||
:::: '''16 O2k-Chambers (8 O2k)''' | |||
:::: {{Template:Oroboros Ecosystem Partner}} | |||
:::: The '''DSL-Oroboros Research Laboratory''' at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells. | |||
:: ''' | :::: '''Oroboros Instruments''' collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions. | ||
=== | === Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory === | ||
{| style="color: black; width: 90%" | |||
|- | |||
| style="text-align: center; background-color: #ffffff" | '''[[Oroboros Laboratories |DSL-Oroboros Research Laboratory]]''' | |||
=== | '''Medical University of Innsbruck''' | ||
| style="text-align: center; background-color: #EFF5FB" | <gallery heights="165px" mode="nolines" perrow="6" widths="192px"> | |||
Image:MeszarosA.JPG|'''[[Meszaros A|András Mészáros]]''', MD., PhD.<br/> - Mitochondrial Medicine Visionary | |||
Image:David.jpg|'''[[Harrison DK| David Harrison]]''', Doz., PhD. - Independent scientific consultant | |||
Image:MittelbergerC.JPG|'''[[Mittelberger C| Christa Mittelberger]]'''<br/> Student | |||
Image:UnterwegerJ.JPG|'''[[Unterweger J| Jasmin Unterweger]]'''<br/> Student | |||
Image:ReyerB.JPG|'''[[Reyer BA| Beatrix Ann Reyer]]'''<br/> Student | |||
Image:HofmannJ.jpg|'''[[Hofmann J| Julia Hofmann]]'''<br/> Student | |||
Image:Profile-icon-9.png|'''[[Buch M| Madita Buch]]'''<br/> Student | |||
</gallery> | |||
|} | |||
=== | == OXPHOS analysis == | ||
:::: | :::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using | ||
::::* coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions; | |||
:: | ::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]). | ||
::::» [[Projects|'''Current projects''']] | |||
:::: [[ | ::::» '''[[Partners in the Oroboros Ecosystem]]''' | ||
::::» [[Oroboros Laboratories: visiting scientists |'''Visiting scientists''']] | |||
Revision as of 15:04, 17 February 2020
Oroboros Laboratory
- Mitochondria and Cell Research - the Oroboros Ecosystem
- Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab
- Scientists with a gentle touch of entrepreneurship
Oroboros O2k-Laboratory
- 48 O2k-Chambers (24 O2k)
- The Oroboros O2k-Laboratory ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project MitoCom Tyrol in June 2013. It comprises 140 sqm which are divided into the office and research areas.
- Twenty four O2k instruments for HRR (48 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
- Two additional O2ks (4 chambers) are devoted to instrumental development.
- According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world.
- » More details: Oroboros Support
Collaborators in the Oroboros Ecosystem
| Collaborations |
|
The DSL-Oroboros Research Laboratory
- 16 O2k-Chambers (8 O2k)
- - a partner in the Oroboros Ecosystem
- The DSL-Oroboros Research Laboratory at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells.
- Oroboros Instruments collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.
Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory
| DSL-Oroboros Research Laboratory
Medical University of Innsbruck |
|
OXPHOS analysis
- SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
- coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
- various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).
