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Difference between revisions of "Oroboros Laboratory"

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{{Template:OROBOROS header page name}}
{{Template:OROBOROS header page name}}
[[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]]
{{Template:OROBOROS Slogan}}
[[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
 
__TOC__
:::: <big>'''Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab'''</big>
== About us ==


The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas.
:::: <big>'''Scientists with a gentle touch of entrepreneurship'''</big>
[[File:Lab in action.jpg|right|500px|Oroboros Laboratory]]


=== O2k instruments ===
[[File:OROBOROS MitoFit Lab IMG 7638.JPG|right|500px |Lisi & Yvonne, MitoFit Laboratory]]
'''28 O2k-Chambers (14 O2k)'''


* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.


* Two additional O2ks (4 chambers) are devoted to instrumental development.
<!--[[File:OROBOROS MitoFit-Lab.jpg|left|240px |Oroboros O2k-Laboratory]]-->


__TOC__


''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]]
== Oroboros O2k-Laboratory ==
:::: '''40 O2k-Chambers (20 O2k)'''


:::: The '''Oroboros O2k-Laboratory''' ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]] in June 2013. It comprises 140 sqm which are divided into the office and research areas.


=== OXPHOS analysis ===
::::* Twenty O2k instruments for HRR (40 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.


SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
::::* Two additional O2ks (4 chambers) are devoted to instrumental development.


* a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
::::* According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world.


* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).
::::» ''More details:'' [[O2k-technical_support_and_open_innovation|Oroboros Support]]




=== The MitoFit Centre of Excellence ===
=== Collaborators in the Oroboros Ecosystem ===
{| style="color: black;  width: 90%"
|-
| style="text-align: center;  background-color: #EFF5FB" | '''Collaborations'''
| style="text-align: center;  background-color: #ffffff" |
<gallery heights="165px" mode="nolines" perrow="6" widths="192px">
Image:FischerC.JPG|'''[[Fischer C| Christine Fischer]]''', MSc., PhD. student
File:Lisa Bergmeister.jpg|'''[[Bergmeister L|Lisa Bergmeister]]''', PhD. student - [[SPIN]]
File:Profile-icon-9.png|'''[[Torres-Quesada O|Omar Torres-Quesada, PhD]]''', PostDoc - [[MitoKIN]]
</gallery>
|}


OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.


According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.


[[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]]


== The DSL-Oroboros Research Laboratory ==
:::: '''16 O2k-Chambers (8 O2k)'''
:::: {{Template:Oroboros Ecosystem Partner}}


:::: The '''DSL-Oroboros Research Laboratory''' at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells.
:::: '''Oroboros Instruments''' collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.


== Current projects and collaborators ==
:: '''The [[OROBOROS_Company#The_OROBOROS-Team |OROBOROS-Team]] collaborates with:'''


=== Pharmacology: mitochondrial function in brain tissues ===
=== Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory ===
[[Image:Johannes.jpg|left|90px|Johannes Burtscher]]
:: '''[[Burtscher J|Johannes Burtscher]]''', Mag. PhD
:: FWF projects W1206-B05 and I-977-B24.
:: Project leader: A.Univ. Prof. Dr. Christian Schwarzer, Dept. Pharmacology, Medical University Innsbruck, Austria
<br />


=== Internal Medicine ===
{| style="color: black;  width: 90%"
[[Image:VolaniC.JPG|left|90px|Chiara Volani]]
|-
:::: '''[[Volani C| Chiara Volani]]''', [mailto:c_volani@yahoo.com c_volani@yahoo.com]
| style="text-align: center;  background-color: #ffffff" | '''[[Oroboros Laboratories |DSL-Oroboros Research Laboratory]]'''  
:::: Project leader: Prof. Dr. Günther Weiss, Dept. of Internal Medicine, Medical University Innsbruck, Austria
<br />


'''Medical University of Innsbruck'''
| style="text-align: center;  background-color: #EFF5FB" | <gallery heights="165px" mode="nolines" perrow="6" widths="192px">
Image:MeszarosA.JPG|'''[[Meszaros A|András Mészáros]]''', MD., PhD.<br/> - Mitochondrial Medicine Visionary
Image:David.jpg|'''[[Harrison DK| David Harrison]]''', Doz., PhD. - Independent scientific consultant
Image:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva Ana Carolina| Ana Carolina Bastos Sant'Anna Silva ]]'''<br/> PhD Student
Image:MittelbergerC.JPG|'''[[Mittelberger C| Christa Mittelberger]]'''<br/> Student


=== MitoFit project ===
Image:UnterwegerJ.JPG|'''[[Unterweger J| Jasmin Unterweger]]'''<br/> Student
Image:ReyerB.JPG|'''[[Reyer BA| Beatrix Ann Reyer]]'''<br/> Student
Image:HofmannJ.jpg|'''[[Hofmann J| Julia Hofmann, MSc]]'''<br/> PhD student
File:Van Appeldorn P.jpg|'''[[Van Appeldorn Paula]]''' <br/> Student
File:Hoelzl K.jpg|'''[[Hoelzl Katharina]]''' <br/> Student
File:RehseN.jpg|'''[[Rehse Nikolas]]''' <br/> Student
Image:Profile-icon-9.png|'''[[Buch M| Madita Buch]]'''<br/> Student
Image:Profile-icon-9.png|'''[[Schipplick F| Florian Schipplick]]'''<br/> Student
Image:Profile-icon-9.png|'''[[Nardin F| Florian Nardin]]'''<br/> PhD Student
Image:Profile-icon-9.png|'''[[Sonnberger Lorenz| Lorenz Sonnberger]]'''<br/> Student
</gallery>


:::: '''[[Hoppel F|Florian Hoppel]]''', MSc.
|}
:::: PhD student (discontinued)
:::: Institute for Sports Science and OROBOROS: [[K-Regio MitoFit]]
[[Image:MitoFit.jpg|100px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]
<br/><br/>


== OXPHOS analysis ==


=== Oncotyrol ===
:::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
:::: '''Prostate cancer'''
:::: The [[Oncotyrol| Oncotyrol]] research project has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue, and comparison of primary tissue with cultured cells. - [http://oroboros.at/index.php?id=oncotyrol Oncotyrol]
:::: Project leader: A.Univ. Prof. Mag. Dr. Helmut Klocker, Dept. Urology, Medical University Innsbruck, Austria


::::'''[[Weber A| Anja Weber]]''', Mag., PhD student - [mailto:anja.weber@i-med.ac.at  anja.weber@i-med.ac.at]
::::* coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]).


[[Image:SchoepfB.jpg|left|90px]]
:: '''[[Schoepf B|Bernd Schoepf]]''', Mag., PhD student -
<br/>
<br/>
<br/>


::::» [[Projects|'''Current projects''']]
::::» '''[[Partners in the Oroboros Ecosystem]]'''
::::» [[Oroboros Laboratories: visiting scientists |'''Visiting scientists''']]


=== Visiting scientists ===
== Keywords ==
:::: [[OROBOROS_MitoFit_lab:_visiting_scientists |'''» Visiting scientists in the MitoFit lab''']]
{{Keywords: Oroboros Laboratories}}

Revision as of 12:13, 25 September 2020

                



Oroboros Laboratory

Mitochondria and Cell Research - the Oroboros Ecosystem
Two labs - one team: Oroboros O2k-Laboratory and DSL-Oroboros Research Lab
Scientists with a gentle touch of entrepreneurship
Oroboros Laboratory



Oroboros O2k-Laboratory

40 O2k-Chambers (20 O2k)
The Oroboros O2k-Laboratory ('Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, and likely the best equipped lab for high-resolution respirometry (HRR) worldwide. The O2k-Lab was opened during the course of the K-Regio project MitoCom Tyrol in June 2013. It comprises 140 sqm which are divided into the office and research areas.
  • Twenty O2k instruments for HRR (40 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
  • Two additional O2ks (4 chambers) are devoted to instrumental development.
  • According to the concept of open innovation, the Oroboros O2k-Laboratory welcomes visiting researchers from all over the world.
» More details: Oroboros Support


Collaborators in the Oroboros Ecosystem

Collaborations



The DSL-Oroboros Research Laboratory

16 O2k-Chambers (8 O2k)
- a partner in the Oroboros Ecosystem
The DSL-Oroboros Research Laboratory at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particularly devoted to mitochondrial research on human biopsies, cells isolated from blood samples, and cultured cells.
Oroboros Instruments collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.


Bioenergetics Adventurers in the DSL-Oroboros Research Laboratory

DSL-Oroboros Research Laboratory

Medical University of Innsbruck

OXPHOS analysis

SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
  • coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
  • various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).


» Current projects
» Partners in the Oroboros Ecosystem
» Visiting scientists

Keywords


Questions.jpg


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