https://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&feed=atom&action=historyOxygen calibration - DatLab - Revision history2024-03-29T11:58:42ZRevision history for this page on the wikiMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=221103&oldid=prevSpitzer Gudrun at 08:15, 7 October 20212021-10-07T08:15:30Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Calibration parameters are automatically transferred to experimental DatLab files'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Calibration parameters are automatically transferred to experimental DatLab files'''</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Step <span style="background:red">4</span>''': When a new DatLab file is opened any time later, the latest calibration parameters are automatically applied in the new DatLab file — independent of maintaining the O2k in operation or switching it off, and independent of maintaining DatLab active or re-opening it after exit.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Step <span style="background:red">4</span>''': When a new DatLab file is <ins style="font-weight: bold; text-decoration: none;">recorded <!--</ins>opened<ins style="font-weight: bold; text-decoration: none;">--> </ins>any time later, the latest calibration parameters are automatically applied in the new DatLab file — independent of maintaining the O2k in operation or switching it off, and independent of maintaining DatLab active or re-opening it after exit.</div></td></tr>
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</table>Spitzer Gudrunhttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=217334&oldid=prevKomlodi Timea at 13:58, 27 April 20212021-04-27T13:58:41Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Instrumental background oxygen flux - general ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Instrumental background oxygen flux - general ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental background oxygen flux derives from oxygen fluxes into or out of the respirometer chamber that are not directly related to respiratory activity of the sample. These fluxes are caused by back diffusion into the chamber at low oxygen pressure, oxygen diffusion out of the chamber at elevated oxygen levels, and oxygen consumption by the polarographic oxygen sensor (OroboPOS). Correction for instrumental background oxygen flux is a standard in high-resolution respirometry, <del style="font-weight: bold; text-decoration: none;">automatically </del>performed by DatLab. Background measurements provide quality control of instrument function. In the Oroboros O2k, background corrections are usually within a few % of experimental flux over the entire experimental oxygen range. At minimum activities, however, even the small background effects become significant and require compliance to standard operating procedures described here.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental background oxygen flux derives from oxygen fluxes into or out of the respirometer chamber that are not directly related to respiratory activity of the sample. These fluxes are caused by back diffusion into the chamber at low oxygen pressure, oxygen diffusion out of the chamber at elevated oxygen levels, and oxygen consumption by the polarographic oxygen sensor (OroboPOS). Correction for instrumental background oxygen flux is a standard in high-resolution respirometry, performed by DatLab. Background measurements provide quality control of instrument function. In the Oroboros O2k, background corrections are usually within a few % of experimental flux over the entire experimental oxygen range. At minimum activities, however, even the small background effects become significant and require compliance to standard operating procedures described here.</div></td></tr>
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</table>Komlodi Timeahttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=217324&oldid=prevKomlodi Timea at 13:18, 27 April 20212021-04-27T13:18:12Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (<ins style="font-weight: bold; text-decoration: none;">''</ins>1<ins style="font-weight: bold; text-decoration: none;">''</ins>) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (<ins style="font-weight: bold; text-decoration: none;">''</ins>2<ins style="font-weight: bold; text-decoration: none;">''</ins>) zero oxygen calibration; (<ins style="font-weight: bold; text-decoration: none;">''</ins>3<ins style="font-weight: bold; text-decoration: none;">''</ins>) high stability of the POS signal tested for sufficiently long periods of time; (<ins style="font-weight: bold; text-decoration: none;">''</ins>4<ins style="font-weight: bold; text-decoration: none;">''</ins>) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (<ins style="font-weight: bold; text-decoration: none;">''</ins>5<ins style="font-weight: bold; text-decoration: none;">''</ins>) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Calibration window steps.png|right|500px]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Calibration window steps.png|right|500px]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Instrumental calibration'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Instrumental calibration'''</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: It is common practice to save instrumental calibration tests in separate DatLab files, which include (''1'') air calibration and zero oxygen calibration in the same calibration file, or (''2'') air calibration and zero oxygen calibration in different calibration files. '''Step <span style="background:red">1</span>''': Select calibration sections from [[Marks - DatLab |Marks]] (R1, R0) while DatLab is connected to the O2k during an instrumental calibration run. '''Step <span style="background:red">2</span>''': <del style="font-weight: bold; text-decoration: none;">click </del>on <span style="background:lightgrey">Calibrate and copy to clipboard</span> in the O2 calibration window in menu 'Calibration' > 'Oxygen, O2'). '''Step <span style="background:red">3</span>''': <del style="font-weight: bold; text-decoration: none;">the </del>calibration file is saved, and DatLab disconnects from the O2k. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: It is common practice to save instrumental calibration tests in separate DatLab files, which include (''1'') air calibration and zero oxygen calibration in the same calibration file, or (''2'') air calibration and zero oxygen calibration in different calibration files. '''Step <span style="background:red">1</span>''': Select calibration sections from [[Marks - DatLab |Marks]] (R1, R0) while DatLab is connected to the O2k during an instrumental calibration run. '''Step <span style="background:red">2</span>''': <ins style="font-weight: bold; text-decoration: none;">Click </ins>on <span style="background:lightgrey">Calibrate and copy to clipboard</span> in the O2 calibration window <ins style="font-weight: bold; text-decoration: none;">(</ins>in menu 'Calibration' > 'Oxygen, O2'). '''Step <span style="background:red">3</span>''': <ins style="font-weight: bold; text-decoration: none;">Click on <span style="background:lightgrey">Save and disconnect</span> in menu File. The </ins>calibration file is saved, and DatLab disconnects from the O2k. </div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''A calibration can be performed and calibration parameters saved during an experimental run'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''A calibration can be performed and calibration parameters saved during an experimental run'''</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: In the specific case of an experiment that involves an aerobic-anoxic transition, zero oxygen calibration is performed within the experimental file, setting a Mark (R0) on the oxygen signal in the anoxic section, and clicking on <span style="background:lightgrey">Calibrate and copy to clipboard</span> in the O2 calibration window in menu 'Calibration' > 'Oxygen, O2').</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: In the specific case of an experiment that involves an aerobic-anoxic transition, zero oxygen calibration is performed within the experimental file, setting a Mark (R0) on the oxygen signal in the anoxic section, and clicking on <span style="background:lightgrey">Calibrate and copy to clipboard</span> in the O2 calibration window <ins style="font-weight: bold; text-decoration: none;">(</ins>in menu 'Calibration' > 'Oxygen, O2').</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Calibration parameters can be selectively imported from any DatLab file'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''Calibration parameters can be selectively imported from any DatLab file'''</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Step <span style="background:red">5</span>''': In addition, the calibration parameter for air calibration, zero oxygen calibration, or both can be imported from a selected DatLab file. This is particularly important, if a zero calibration is performed after completion and saving an experimental file. '''Step <span style="background:red">6</span>''': A single calibration value (R1 or R0) can be edited manually. Take '''Step <span style="background:red">7</span>''' to apply the selected calibration <del style="font-weight: bold; text-decoration: none;">paramters</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Step <span style="background:red">5</span>''': In addition, the calibration parameter for air calibration, zero oxygen calibration, or both can be imported from a selected DatLab file. This is particularly important, if a zero calibration is performed after completion and saving an experimental file. '''Step <span style="background:red">6</span>''': A single calibration value (R1 or R0) can be edited manually. Take '''Step <span style="background:red">7</span>''' to apply the selected calibration <ins style="font-weight: bold; text-decoration: none;">parameters</ins>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Air and zero calibration: O2-calibration_air and zero.DLP ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Air and zero calibration: O2-calibration_air and zero.DLP ===</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::* O2 sensor test and calibration at air saturation and zero oxygen: <del style="font-weight: bold; text-decoration: none;">O2 sensor test and calibration at air saturation and zero oxygen.</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::* O2 sensor test and calibration at air saturation and zero oxygen:</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: This DL-Protocol is identical in the section of air calibration, and continues with zero calibration as follows:</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: This DL-Protocol is identical in the section of air calibration, and continues with zero calibration as follows:</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Air calibration.png|thumb|right|600px|'''Air calibration:''' Oxygen concentration (blue plot; 180 µM; scale from 0 to 250 nmol/mL or 0 to 250 µM) over 1 h after switching on the O2k (Power-O2k P4, chamber A) and setting the experimental temperature at 37 °C, using medium stirred for equilibration with a gas phase of air at 575 m altitude. The red plot is the negative slope of oxygen concentration over time [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] on the right Y-axis, with zero in the middle position. A slope of zero (for uncorrected ‘O2 Slope neg.’) indicates a constant O<sub>2</sub> signal over time. (2014-07-24 P4-01.DLD)]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Air calibration.png|thumb|right|600px|'''Air calibration:''' Oxygen concentration (blue plot; 180 µM; scale from 0 to 250 nmol/mL or 0 to 250 µM) over 1 h after switching on the O2k (Power-O2k P4, chamber A) and setting the experimental temperature at 37 °C, using medium stirred for equilibration with a gas phase of air at 575 m altitude. The red plot is the negative slope of oxygen concentration over time [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] on the right Y-axis, with zero in the middle position. A slope of zero (for uncorrected ‘O2 Slope neg.’) indicates a constant O<sub>2</sub> signal over time. (2014-07-24 P4-01.DLD)]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Zero oxygen calibration may be achieved by chemical or biological depletion of oxygen. (1) Chemical: Following chamber assembly or exchange of the POS membrane, zero solution (Na-dithionite, OroboPOS-Service Kit) is titrated into the chamber, which is part of the automatic TIP2k-supported instrumental background test (MiPNet14.06). (2) Biological: Zero calibration can be performed with mitochondria or cell suspensions that allow complete oxygen depletion.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Zero oxygen calibration may be achieved by chemical or biological depletion of oxygen. (<ins style="font-weight: bold; text-decoration: none;">''</ins>1<ins style="font-weight: bold; text-decoration: none;">''</ins>) Chemical: Following chamber assembly or exchange of the POS membrane, zero solution (Na-dithionite, OroboPOS-Service Kit) is titrated into the chamber, which is part of the automatic TIP2k-supported instrumental background test (MiPNet14.06). (<ins style="font-weight: bold; text-decoration: none;">''</ins>2<ins style="font-weight: bold; text-decoration: none;">''</ins>) Biological: Zero calibration can be performed with mitochondria or cell suspensions that allow complete oxygen depletion.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Air calibration in DatLab ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Air calibration in DatLab ===</div></td></tr>
</table>Komlodi Timeahttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=217320&oldid=prevKomlodi Timea at 12:50, 27 April 20212021-04-27T12:50:38Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the <del style="font-weight: bold; text-decoration: none;">automatic </del>calibration routine using instrumental calibration DL-Protocols in [[DatLab]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
</table>Komlodi Timeahttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=216915&oldid=prevCecatto Cristiane at 07:27, 9 April 20212021-04-09T07:27:40Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:27, 9 April 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental O<sub>2</sub> background tests can be perfomed using the TIP2k or with manual injections. Detailed instructions are provided here: [[MiPNet14.06_Instrumental_O2_background]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental O<sub>2</sub> background tests can be perfomed using the TIP2k or with manual injections. Detailed instructions are provided here: [[MiPNet14.06_Instrumental_O2_background]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Following the preparation of necessary solutions as described in [[MiPNet14.06_Instrumental_O2_background]] and loading and mounting of the syringes (when the TIP2k is used) the appropriate DatLab protocol should be used: [[DL-<del style="font-weight: bold; text-decoration: none;">Protocols#DL-Protocol_collection</del>]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Following the preparation of necessary solutions as described in [[MiPNet14.06_Instrumental_O2_background]] and loading and mounting of the syringes (when the TIP2k is used) the appropriate DatLab protocol should be used: [[<ins style="font-weight: bold; text-decoration: none;">Run </ins>DL-<ins style="font-weight: bold; text-decoration: none;">Protocol/Set O2 limit</ins>]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
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</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=216914&oldid=prevCecatto Cristiane at 06:58, 9 April 20212021-04-09T06:58:31Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# After stabilization of the POS signal, the recorded signal at air saturation, R1, is about 1-3 V (at Gain 1) and a temperature of 25-37 °C. A signal of 1 V corresponds to a signal current of the POS of 1 µA (corresponding to 2 V at Gain 2). Under all experimental conditions, the raw signal must be <10 V. Continue recording for 3-10 min to check for signal stability. You may proceed at this point with an O<sub>2</sub> background test (see below).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# After stabilization of the POS signal, the recorded signal at air saturation, R1, is about 1-3 V (at Gain 1) and a temperature of 25-37 °C. A signal of 1 V corresponds to a signal current of the POS of 1 µA (corresponding to 2 V at Gain 2). Under all experimental conditions, the raw signal must be <10 V. Continue recording for 3-10 min to check for signal stability. You may proceed at this point with an O<sub>2</sub> background test (see below).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:Air calibration.png|thumb|right|600px|'''Air calibration:''' Oxygen concentration (blue plot; 180 µM; <del style="font-weight: bold; text-decoration: none;">full </del>scale <del style="font-weight: bold; text-decoration: none;">50 </del>nmol/<del style="font-weight: bold; text-decoration: none;">ml </del>or <del style="font-weight: bold; text-decoration: none;">50 </del>µM) over 1 h after switching on the O2k (Power-O2k <del style="font-weight: bold; text-decoration: none;">P6</del>, chamber A) and setting the experimental temperature at 37 °C, using medium stirred for equilibration with a gas phase of air at 575 m altitude. The red plot is the negative slope of oxygen concentration over time [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] on the right Y-axis, with zero in the middle position. A slope of zero (for uncorrected ‘O2 Slope neg.’) indicates a constant O<sub>2</sub> signal over time. (2014-07-24 P4-01.DLD)]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:Air calibration.png|thumb|right|600px|'''Air calibration:''' Oxygen concentration (blue plot; 180 µM; scale <ins style="font-weight: bold; text-decoration: none;">from 0 to 250 </ins>nmol/<ins style="font-weight: bold; text-decoration: none;">mL </ins>or <ins style="font-weight: bold; text-decoration: none;">0 to 250 </ins>µM) over 1 h after switching on the O2k (Power-O2k <ins style="font-weight: bold; text-decoration: none;">P4</ins>, chamber A) and setting the experimental temperature at 37 °C, using medium stirred for equilibration with a gas phase of air at 575 m altitude. The red plot is the negative slope of oxygen concentration over time [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] on the right Y-axis, with zero in the middle position. A slope of zero (for uncorrected ‘O2 Slope neg.’) indicates a constant O<sub>2</sub> signal over time. (2014-07-24 P4-01.DLD)]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Zero oxygen calibration may be achieved by chemical or biological depletion of oxygen. (1) Chemical: Following chamber assembly or exchange of the POS membrane, zero solution (Na-dithionite, OroboPOS-Service Kit) is titrated into the chamber, which is part of the automatic TIP2k-supported instrumental background test (MiPNet14.06). (2) Biological: Zero calibration can be performed with mitochondria or cell suspensions that allow complete oxygen depletion.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Zero oxygen calibration may be achieved by chemical or biological depletion of oxygen. (1) Chemical: Following chamber assembly or exchange of the POS membrane, zero solution (Na-dithionite, OroboPOS-Service Kit) is titrated into the chamber, which is part of the automatic TIP2k-supported instrumental background test (MiPNet14.06). (2) Biological: Zero calibration can be performed with mitochondria or cell suspensions that allow complete oxygen depletion.</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l148">Line 148:</td>
<td colspan="2" class="diff-lineno">Line 148:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: <ins style="font-weight: bold; text-decoration: none;">[[</ins>MiPNet06.03 POS-calibration-SOP<ins style="font-weight: bold; text-decoration: none;">]]</ins>. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "Template O2 calibration.xlsx".</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "Template O2 calibration.xlsx".</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l167">Line 167:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:lightgreen">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:lightgreen">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: <ins style="font-weight: bold; text-decoration: none;">[[</ins>MiPNet06.03 POS-calibration-SOP<ins style="font-weight: bold; text-decoration: none;">]]</ins>. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "Template O2 calibration.xlsx".</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "Template O2 calibration.xlsx".</div></td></tr>
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</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=216910&oldid=prevCecatto Cristiane at 15:21, 8 April 20212021-04-08T15:21:38Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:21, 8 April 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: Mark M: J°1 - O2 BG flux ~ air saturation (instrumental background flux after air calibration)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: Mark M: J°1 - O2 BG flux ~ air saturation (instrumental background flux after air calibration)</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::: 1 - Set a mark (J°1) after event <del style="font-weight: bold; text-decoration: none;">2C </del>at a section, where the O2 slope is stable, and observe the signal. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::: 1 - Set a mark (J°1) after event <ins style="font-weight: bold; text-decoration: none;">1C </ins>at a section, where the O2 slope is stable, and observe the signal. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* 'O2 slope neg.' should be within the range of 2 to 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* 'O2 slope neg.' should be within the range of 2 to 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* 'O2 slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> may indicate a biological contamination in the medium or O2k-chamber.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* 'O2 slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> may indicate a biological contamination in the medium or O2k-chamber.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: 3 - Proceed with event 2Dith*.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: 3 - Proceed with event 2Dith*.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::: Event <span style="background:yellow">E: <del style="font-weight: bold; text-decoration: none;">2Dit</del>*</span style> - Manual dithionite titration</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::: Event <span style="background:yellow">E: <ins style="font-weight: bold; text-decoration: none;">2Dith</ins>*</span style> - Manual dithionite titration</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: 1 - Events 2Dith*: Inject fresh Na-dithionite solution ("zero solution") until the O2 signal is reduced close to 0 µM.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::: 1 - Events 2Dith*: Inject fresh Na-dithionite solution ("zero solution") until the O2 signal is reduced close to 0 µM.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* A few minutes are required until a stable "zero" signal is obtained </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::* A few minutes are required until a stable "zero" signal is obtained </div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l147">Line 147:</td>
<td colspan="2" class="diff-lineno">Line 147:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull<ins style="font-weight: bold; text-decoration: none;">-</ins>down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "<ins style="font-weight: bold; text-decoration: none;">Template </ins>O2 calibration.xlsx".</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Zero calibration in DatLab ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Zero calibration in DatLab ===</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l161">Line 161:</td>
<td colspan="2" class="diff-lineno">Line 161:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Only if <span style="background:lightgrey">Mouse Control: Zoom</span style> mode has been activated: Select <span style="background:lightgrey">Mouse Control: Mark</span style> in the Graph menu or press [Ctrl+M].</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Only if <span style="background:lightgrey">Mouse Control: Zoom</span style> mode has been activated: Select <span style="background:lightgrey">Mouse Control: Mark</span style> in the Graph menu or press [Ctrl+M].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Set a mark: Hold [Shift] and click the left mouse button, move the cursor along the time axis, release the left mouse button at the end of the section to be marked. Remove a section of the mark or the total mark: Move the cursor with [Shift+right-click] along the time axis, release the mouse button at the end of the section of the mark to be deleted.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Set a mark: Hold [Shift] and click the left mouse button, move the cursor along the time axis, release the left mouse button at the end of the section to be marked. Remove a section of the mark or the total mark: Move the cursor with [Shift+right-click] along the time axis, release the mouse button at the end of the section of the mark to be deleted.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# Rename the mark: Left mouse click on the bar of the mark. Rename the mark for <del style="font-weight: bold; text-decoration: none;">air </del>calibration as “R0”.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# Rename the mark: Left mouse click on the bar of the mark. Rename the mark for <ins style="font-weight: bold; text-decoration: none;">zero </ins>calibration as “R0”.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''2. Calibrate'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::: '''2. Calibrate'''</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l168">Line 168:</td>
<td colspan="2" class="diff-lineno">Line 168:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull-down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If the temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the corresponding field.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "<ins style="font-weight: bold; text-decoration: none;">Template </ins>O2 calibration.xlsx".</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=216769&oldid=prevCecatto Cristiane at 11:16, 2 April 20212021-04-02T11:16:05Z<p></p>
<a href="https://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=216769&oldid=209266">Show changes</a>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=209266&oldid=prevCecatto Cristiane at 15:44, 5 November 20202020-11-05T15:44:59Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the automatic calibration routine using instrumental <del style="font-weight: bold; text-decoration: none;">calibraiton </del>DL-Protocols in [[DatLab]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|description='''O2 calibration''' is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the automatic calibration routine using instrumental <ins style="font-weight: bold; text-decoration: none;">calibration </ins>DL-Protocols in [[DatLab]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l148">Line 148:</td>
<td colspan="2" class="diff-lineno">Line 148:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:yellow">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull down button and select the appropriate mark <span style="background:lightgrey">R1</span style>. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the <del style="font-weight: bold; text-decoration: none;">coresponding </del>field.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the <ins style="font-weight: bold; text-decoration: none;">corresponding </ins>field.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Click on <span style="background:lightgrey">Calibrate and copy to clipboard</span> before closing the calibration window. Thereby, calibration is activated and calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l166">Line 166:</td>
<td colspan="2" class="diff-lineno">Line 166:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:lightgreen">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Open the DatLab calibration window by double clicking on <span style="background:lightgreen">O2 calib.</span style> for O2k-chamber A or B in the O2k-status line (bottom left and right). Alternatively, press [F5] to open the calibration window for the active plot.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on the pull<ins style="font-weight: bold; text-decoration: none;">-</ins>down button and select the appropriate mark <span style="background:lightgrey">R0</span style>. Many times the zero calibration value is used from a previous experiment. The displayed temperature and pressure are without influence on the calibration calculations for zero oxygen. The stable zero signal, R0, should be <2 % of the signal at air saturation, but <5 % is acceptable. Most importantly, the zero signal must be stable. The average voltage (Raw signal [V]) recorded over the marked section is shown in the corresponding field on the right. The corresponding signal stability is displayed as the uncorrected negative slope of the signal during calibration in [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>]. Temperature and barometric pressure are displayed as measured over the marked section. Calibration values R1 and R0 can be edited numerically, without exerting an influence on c1. If temperature or barometric pressure are edited, then c1 is recalculated for the changed conditions.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the <del style="font-weight: bold; text-decoration: none;">coresponding </del>field.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# If not set previously, enter the oxygen solubility factor of the medium, ''F''<sub>M</sub>, relative to pure water. For more info on this factor: MiPNet06.03 POS-calibration-SOP. For documentation purposes, enter the name of the experimental incubation medium in the <ins style="font-weight: bold; text-decoration: none;">corresponding </ins>field.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Left click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style>. After clicking on this button all changes in the entire calibration window are applied. The entire plot of oxygen concentration is re-calibrated [µM = nmol/mL], and the corresponding negative slope or volume-specific oxygen flux [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] is now based on this new calibration. Calibration parameters are automatically copied to clipboard for entry into the spreadsheet "O2 calibration.xlsx".</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l188">Line 188:</td>
<td colspan="2" class="diff-lineno">Line 188:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# After switching on the O2k, every day: air calibration, stirrer test, quality control ([[O2_calibration_-_DatLab#Calibration_and_quality_control |Calibration and quality control]]).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# After switching on the O2k, every day: air calibration, stirrer test, quality control ([[O2_calibration_-_DatLab#Calibration_and_quality_control |Calibration and quality control]]).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Zero oxygen calibration: from time to time over weeks; bracketing zero oxygen calibrations when working at low oxygen ([[O2_calibration_-_DatLab#Zero_calibration_-_general_description |Zero calibration]]).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Zero oxygen calibration: from time to time over weeks; bracketing zero oxygen calibrations when working at low oxygen ([[O2_calibration_-_DatLab#Zero_calibration_-_general_description |Zero calibration]]).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::# After application of a new membrane and O2-sensor service: in some cases, the signal of the OroboPOS improves (higher signal stability, less noise, shorter response time)<del style="font-weight: bold; text-decoration: none;">, </del>when the O2k remains switched on <del style="font-weight: bold; text-decoration: none;">over night </del>(O2k-Chambers filled with 70 % EtOH).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::# After application of a new membrane and O2-sensor service: in some cases, the signal of the OroboPOS improves (higher signal stability, less noise, shorter response time) when the O2k remains switched on <ins style="font-weight: bold; text-decoration: none;">overnight </ins>(O2k-Chambers filled with 70 % EtOH <ins style="font-weight: bold; text-decoration: none;">or H<sub>2</sub>O if the traces will be evaluated in the morning</ins>).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# For [[O2_calibration_-_DatLab#Calibration_and_quality_control |O2k-Quality Control]] (O2k-QC) of instrumental performance.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# For [[O2_calibration_-_DatLab#Calibration_and_quality_control |O2k-Quality Control]] (O2k-QC) of instrumental performance.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Before an O2k-chamber test ([[O2_calibration_-_DatLab#Instrumental_background_oxygen_flux_-_general |Instrumental O2 background test]]).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::# Before an O2k-chamber test ([[O2_calibration_-_DatLab#Instrumental_background_oxygen_flux_-_general |Instrumental O2 background test]]).</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l203">Line 203:</td>
<td colspan="2" class="diff-lineno">Line 203:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental O<sub>2</sub> background tests can be perfomed using the TIP2k or with manual injections. Detailed instructions are provided here: [[MiPNet14.06_Instrumental_O2_background]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Instrumental O<sub>2</sub> background tests can be perfomed using the TIP2k or with manual injections. Detailed instructions are provided here: [[MiPNet14.06_Instrumental_O2_background]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Following the preparation of necessary solutions as <del style="font-weight: bold; text-decoration: none;">decribed </del>in [[MiPNet14.06_Instrumental_O2_background]] and loading and mounting of the syringes (when the TIP2k is used) the appropriate DatLab protocol should be used: [[DL-Protocols#DL-Protocol_collection]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Following the preparation of necessary solutions as <ins style="font-weight: bold; text-decoration: none;">described </ins>in [[MiPNet14.06_Instrumental_O2_background]] and loading and mounting of the syringes (when the TIP2k is used) the appropriate DatLab protocol should be used: [[DL-Protocols#DL-Protocol_collection]]</div></td></tr>
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</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Oxygen_calibration_-_DatLab&diff=207845&oldid=prevKomlodi Timea at 09:13, 5 October 20202020-10-05T09:13:19Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:13, 5 October 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Answer 1: Different initial oxygen levels after switching on the instrument are due to missing calibration. Sensors need to be [[MiPNet06.03_POS-calibration-SOP | air calibrated]] on each experimental day to account for changes in barometric pressure or to <del style="font-weight: bold; text-decoration: none;">accomodate </del>changes in experimental temperature or medium.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Answer 1: Different initial oxygen levels after switching on the instrument are due to missing calibration. Sensors need to be [[MiPNet06.03_POS-calibration-SOP | air calibrated]] on each experimental day to account for changes in barometric pressure or to <ins style="font-weight: bold; text-decoration: none;">accommodate </ins>changes in experimental temperature or medium.</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: Answer 2: The zero voltage in chamber A is at 0.8996 V, which is too high. Zero voltage should not exceed <del style="font-weight: bold; text-decoration: none;">10 </del>% (usually lower than <del style="font-weight: bold; text-decoration: none;">5 </del>%) of the voltage obtained at air saturation (R1). You should perform a complete [[MiPNet19.18B_POS-service | POS service]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Answer 2: The zero voltage in chamber A is at 0.8996 V, which is too high. Zero voltage should not exceed <ins style="font-weight: bold; text-decoration: none;">5 </ins>% (usually lower than <ins style="font-weight: bold; text-decoration: none;">2 </ins>%) of the voltage obtained at air saturation (R1). You should perform a complete [[MiPNet19.18B_POS-service | POS service]].</div></td></tr>
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</table>Komlodi Timea