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Difference between revisions of "Oxygen solubility factor"

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{{MitoPedia
{{MitoPedia
|abbr=''F''<sub>M</sub>
|abbr=''F''<sub>M</sub>
|description=The '''oxygen solubility factor''' of the incubation medium, ''F''<sub>M</sub>, expresses the effect of the salt concentration on [[oxygen solubility]] relative to pure water.  In mitochondrial respiration medium [[MiR06]], ''F''<sub>M</sub> is 0.92 determined at 30 and 37 °C, and ''F''<sub>M</sub> is 0.89 in serum at 37 °C. ''F''<sub>M</sub> for other media may be estimated using Table 4 in [[MiPNet06.03]]. For this purpose KCl based media can be described as "seawater" of varying salinity.
|description=The '''oxygen solubility factor''' of the incubation medium, ''F''<sub>M</sub>, expresses the effect of the salt concentration on [[oxygen solubility]] relative to pure water.  In mitochondrial respiration medium [[MiR05]], [[MiR05-Kit]] and [[MiR06]], ''F''<sub>M</sub> is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). ''F''<sub>M</sub> varies depending on the temperature and composition of the medium. To calculate the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]. For other media, FM may be estimated using Table 4 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]. For this purpose KCl based media can be described as "seawater" of varying salinity.
|info=[[MiPNet06.03]]
 
|info=[[MiPNet06.03 POS-calibration-SOP]]
 
}}
}}
== Keywords ==
{{Keywords: Concentration and pressure}}
{{Template:Keywords: Oxygen signal}}
[[Image:MiPNet06.03_Table.jpg|right|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. ''F''<sub>M</sub> has been determined for [[MiR05]] at 30 °C and 37 °C, and is assumed to be identical at 25 °C, and identical in [[MiR06]].]]
{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry
|mitopedia method=Respirometry
}}
{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=DatLab
}}
{{MitoPedia topics
|mitopedia topic=Medium
}}
}}
{{Labeling
{{Labeling
|topics=Oxygen
|instruments=Theory
|instruments=Theory
}}
}}
[[Category:MitoPedia]]
[[Category:MitoPedia]]
{{#set:Technical service=POS calibration}}

Revision as of 06:56, 5 March 2020


high-resolution terminology - matching measurements at high-resolution


Oxygen solubility factor

Description

The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. In mitochondrial respiration medium MiR05, MiR05-Kit and MiR06, FM is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). FM varies depending on the temperature and composition of the medium. To calculate the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in MiPNet06.03. For other media, FM may be estimated using Table 4 in MiPNet06.03. For this purpose KCl based media can be described as "seawater" of varying salinity.

Abbreviation: FM

Reference: MiPNet06.03 POS-calibration-SOP

Keywords


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cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C, and identical in MiR06.


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab 


MitoPedia topics: Media for respirometry 


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HRR: Theory