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PBI-Shredder O2k-Set

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PBI-Shredder O2k-Set

O2k-Catalogue

Description PBI-Shredder HRR-Set: Auxiliary HRR-Tool for tissue homogenate preparation; the Shredder-Kit Box contains the heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger, two lithium ion batteries, Shredder-Tube Cap Tool and Shredder-Tube Ram Tool. The PBI-Shredder HRR-Set includes the Shredder-Kit Box with 1 box of 100 Shredder-Tubes FT500-PS, 1 box of 100 Shredder-Tubes FT500-PMS100, a pair of sharp forceps for tissue dissection and a pair of scissors.

OROBOROS INSTRUMENTS: world-wide distributor.

Product ID 13200-04
Type O2k, PBI-Shredder, Catalogue
Link PBI-Shredder @OROBOROS, Manual_PBI-Shredder, O2k-Catalogue: PBI-Shredder, Purchase Order @OROBOROS, Shredder vs. Fibres
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PBI-Shredder HRR-Set.JPG

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PBI-Shredder HRR-Set consists of

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>>> Go to O2k-Catalogue OROBOROS, or Order @OROBOROS

Instructions

Using the Shredder-Tube Cap Tool, a serrated [[Shredder-Ram}} is inserted into one of the Shredder-Tubes, pressing the sample between the serratged surface of the Shredder-Ram and the lysis disk of the tube. Ice-cold mitochondrial medium (200-700 µl MiR06 or MiR06Cr) is then added to the opposite Cap side of the tube, which is closed by a Shredder-Screw Cap, again using the Shredder-Tube Cap Tool. The filled Shredder-Tube is placed into the pre-cooled Shredder Base, with the Ram side facing downwards, and the SG3 driver is placed onto the Cap. The force setting lever is placed into the position (1-low, 2-medium), and shredding for 20-30 s is sufficient for several different tissues. The processed homogenate is sampled from the Shredder-Tube by unscrewing the Shredder-Screw Cap using the Shredder-Tube Cap Tool and removing the cap, for transfer of the sample into the O2k-Chamber.


Advantages of the PBI-Shredder

  • Low shear mechanical device for gentle, rapid and safe disruption of tissues;
  • Three position lever for setting reproducible force to the sample during the shredding process;
  • Gentle enough for isolating intact, functional mitochondria;
  • Powerful enough to rapidly break apart difficult samples;
  • Standardized preparations of high quality;
  • Enables reproducible results;
  • Easy handling, especially for beginners;
  • Processing containers:
> Standard tubes for ambient pressure processing
> Closed containers help to ensure safety throughout the entire sample preparation process;
> Excellent for collection, storage, transport and processing
  • High quality of functional mitochondria is obtained from skeletal muscel and kidney as evaluated by high resolution respirometry:
Gross VS, Greenberg HK, Baranov SV, Carlson GM, Stavrovskaya IG, Lazarev AV, Kristal BS (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Analyt Biochem 418: 213-223.

Traditional methods for homogenization

  • Homogenizer-based protocols that require extensive operator experience (highly skilled personnel) to obtain reproducible high-quality preparations
  • These methods limit dissemination, impede scale-up, and contribute to difficulities in reproducing experimental results over time and across laboratories
  • During manual homogenization it is difficult to know when sufficient tissue disruption has occured - overhomogenization!
  • The current protocols rely on Dounce, Potter-Elvehjem, and rotor/stator shearing homogenization:
Pallotti F, Lenaz G (2007) Isolation and subfractionation of mitochondria from animal cells and tissue culture lines. Methods Cell Biol 80: 3-44.
Frezza C, Cipolat S, Scorrano L (2007) Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat Protoc 2: 287-295.
Kaufmann P, Török M, Zahno A, Waldhauser KM, Brecht K, Krähenbühl S (2006) Toxicity of statins on rat skeletal muscle mitochondria. Cell Mol Life Sci 63: 2415-2425.