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Palmitate is a term for the salts and esters of palmitic acid (CH3(CH2)14COOH). Palmitic acid is the first fatty acid produced during fatty acid synthesis and the precursor to longer fatty acids. Palmitate negatively feeds back on acetyl-CoA carboxylase (ACC), which is responsible for converting acetyl-CoA to malonyl-CoA, which in turn is used to add to the growing acyl chain, thus preventing further palmitate generation. In order to dissolve the water-insoluble sodium palmitate, BSA is needed to form the water-soluble compound called palmitate:BSA.

Abbreviation: Paa

Application in HRR

Paa: Sodium palmitate (Sigma P 9767, 5 g, store at 4 Β°C; M = 278.41 gΒ·mol-1)
Preparation of 150 mM NaCl stock solution.
  1. Weigh 438.3 mg NaCl directly into a glass beaker.
  2. Add 50 mL H2O and mix it together.
Preparation of 1.66 mM BSA stock solution.
  1. Weigh 5 g BSA. (66kDa=66000Da=66000g/mol)
  2. Add 45.6 mL 150 mM NaCl solution and mix it.
Preparation of 10 mM sodium palmitate solution.
  1. Weigh 27.84 mg of sodium palmitate directly into a 10 mL glass beaker.
  2. Add 10 mL H2O.
  3. Add 10 mL 1.66 mM BSA stock solution.
  4. Mix it for 1 hour at 70 Β°C.
  5. Store at -20 Β°C in Eppendorf tubes.

MitoPedia topics: Substrate and metabolite 



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