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Difference between revisions of "Pasdois 2003 J Bioenerg Biomembr"

From Bioblast
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|organism=Rat
|organism=Rat
|tissues=Neurons; Brain
|tissues=Neurons; Brain
|preparations=Permeabilized Cell or Tissue; Homogenate
|preparations=Intact Cell; Cultured; Primary
|enzymes=TCA Cycle and Matrix Dehydrogenases, Complex I
|enzymes=TCA Cycle and Matrix Dehydrogenases, Complex I
|topics=Respiration; OXPHOS; ETS Capacity
|topics=Respiration; OXPHOS; ETS Capacity
}}
}}

Revision as of 10:39, 6 September 2011

Publications in the MiPMap
Pasdois P, Deveaud C, Voisin P, Bouchaud V, Rigoulet M, Beauvoit B (2003) Contribution of the phosphorylable complex I in the growth phase-dependent respiration of C6 glioma cells in vitro. J. Bioenerg. Biomembr. 35: 439-450.

Β» PMID: 14740892

Pasdois P, Deveaud C, Voisin P, Bouchaud V, Rigoulet M, Beauvoit B (2003) J. Bioenerg. Biomembr.

Abstract: The energy metabolism of rat C6 glioma cells was investigated as a function of the growth phases. Three-dimensional cultures of C6 cells exhibited diminished respiration and respiratory capacity during the early growth phase, before reaching confluence. This decrease in respiration was neither due to changes in the respiratory complex content nor in the mitochondrial mass per se. Nevertheless, a quantitative correlation was found between cellular respiration and the rotenone-sensitive NADH ubiquinone oxidoreductase (i.e. complex I) activity. Immunoblot analysis showed that phosphorylation of the 18 kDa-subunit of this complex was associated with the growth-phase dependent modulation of complex I and respiratory activity in C6 cells. In addition, by using forskolin or dibutyryl cAMP, short-term activation of protein kinases A of C6 cells correlated with increased phosphorylation of the 18-kDa subunit of complex I, activated NADH ubiquinone oxidoreductase activity and stimulated cellular respiration. These findings suggest that complex I of C6 glioma cells is a key regulating step that modulates the oxidative phosphorylation capacity during growth phase transitions. β€’ Keywords: Complex I, NADH ubiquinone oxidoreductase, cAMP-dependent phosphorylation, Respiration, C6 glioma

β€’ O2k-Network Lab: FR_Bordeaux_Rigoulet M, FR_Pessak_Beauvoit B


Labels:


Organism: Rat  Tissue;cell: Neurons; Brain"Neurons; Brain" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: TCA Cycle and Matrix Dehydrogenases"TCA Cycle and Matrix Dehydrogenases" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Complex I  Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k