Pesta 2023 MiP2023

Pesta Dominik (2023) Respiratory capacity of skeletal muscle and peripheral blood mononuclear cells of male and female individuals with type 2 diabetes. MiP2023 Obergurgl AT.

Link: MiP2023 Obergurgl AT

Buescher F-M, Schrage-Knoll Irmtrud, Bohmeier Maria, Kaiser-Stolz C, Kramme J, Rittweger J, Pesta Dominik (2023)

Event: MiP2023 Obergurgl AT

Introduction: Skeletal muscle mitochondrial function is altered in insulin resistant states. Its assessment, however, requires invasive muscle biopsies to obtain viable tissue for functional mitochondrial analysis. Blood cell-based bioenergetics potentially reflects systemic mitochondrial function. Here, we characterized respiratory capacity of skeletal muscle mitochondria and peripheral blood mononuclear cells (PBMCs) from patients with type 2 diabetes and assessed whether the latter reflect muscle mitochondrial respirometric measures. Methods: For that purpose, 20 patients with type 2 diabetes (30% female, 57±9 years, BMI 28±4 kg/m2) participated in this study. We obtained muscle biopsies from the M. vastus lateralis and venous blood samples to isolate PBMCs. High-resolution respirometry was performed in duplicate to assess mitochondrial respiration from permeabilized muscle fibers and PBMCs using an established SUIT-protocol. Results and Discussion: Combined NADH-linked (N) electron transfer and succinate-linked (S) OXPHOS capacity was 59.4±13.0 pmol/(s*mg) for muscle and 16.6±5.3 pmol/(s*106 cells) for PBMCs. NS-OXPHOS capacity was not different between females and males for muscle (66.5±9.5 vs 56.3±13.0 pmol/(s*mg), p=0.10) or PBMCs (19.5±5.3 vs 15.3±5.0 pmol/(s*106), p=0.10), respectively. While PBMC mitochondrial function was not correlated with skeletal muscle respiratory function across several respiratory states (all p>0.05), muscle NS-OXPHOS capacity correlated negatively with diabetes disease duration (r=-0.50, p=0.02). These results suggest that there are no sex-specific differences with regard to muscle and PBMC mitochondrial function in individuals with type 2 diabetes. While bioenergetic phenotypes in PBMCs do not reflect muscle mitochondrial function in this cohort, diabetes disease duration negatively associates with muscle mitochondrial function.

• O2k-Network Lab: DE Cologne Pesta D

Labels: Pathology: Diabetes 

Tissue;cell: Skeletal muscle, Blood cells 

Event: Oral 

Acknowledgements: We wish to thank all patients and staff that participated in or helped with this study. Author contributions: Data collection was performed by FMB, IS, MB, CKS, JK, JR and DP. Data analysis and evaluation was performed by FMB and DP. All authors contributed to writing the abstract. Conflicts of interest: The authors have no conflict of interest to declare. Data availability: Data are available from the authors upon reasonable request after publication.

Büscher F-M1, Schrage I1, Bohmeier M1, Kaiser-Stolz C1, Kramme J1,2, Rittweger J1, Pesta D1,2,3 1German Aerospace Center (DLR), Institute of Aerospace Medicine, D-51147 Cologne, Germany. 2Center for Endocrinology, Diabetes and Preventive Medicine (CEDP), University Hospital Cologne, D-50937 Cologne, Germany. 3Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, D-50931 Cologne, Germany. Corresponding author: Dominik.Pesta@dlr.de