Rasmussen 2003 Eur J Physiol

Rasmussen UF, Krustrup P, Kjaer M, Rasmussen HN (2003) Human skeletal muscle mitochondrial metabolism in youth and senescence: no signs of functional changes in ATP formation and mitochondrial oxidative capacity. Pflugers Arch – Eur J Physiol 446:270-78.

» PMID: 12739165

Rasmussen UF, Krustrup P, Kjaer M, Rasmussen HN (2003) Eur J Physiol

Abstract: The mitochondrial theory of ageing was tested. Isolated mitochondria from the quadriceps muscle from normal, healthy, young (age 20+ years, n=12) and elderly (70+ years, n=11) humans were studied in respiratory experiments and the data expressed as activities of the muscle. In each group, the subjects exhibited a variation of physical activity but, on average, the groups were representative for their age with maximum O(2) consumption rate of 50+/-9 and 34+/-13 ml min(-1) kg(-1) (mean+/-SD), respectively. Thirteen different activities were assayed. alpha-Glycerophosphate oxidation was lower in the 70+ group (38%, P~0.001), as was the respiratory capacity for fatty acids (19%, P~0.03). The remaining eleven activities, including those of the central bioenergetic reactions, were not lower in the 70+ group. Pyruvate and alpha-ketoglutarate dehydrogenase activities (i.e. the tricarboxylic acid cycle turnover) and the respiratory chain activity could all account for ~14 mmol O(2) min(-1) kg(-1) muscle (37 degrees C). The capacity for aerobic ATP synthesis was ~35 mmol ATP min(-1) kg(-1). The mitochondrial capacities were far in excess of whole-body performance. They were related to physical activity, but not to age. The mitochondrial theory of ageing, which attributes the age-related decline of muscle performance to decreased mitochondrial function, is incompatible with these results. • Keywords: Age effects, Ageing, Human skeletal muscle, Isolated mitochondria, Oxidative phosphorylation, Oxygen uptake, Quadriceps muscle, Respiration • Bioblast editor: Gnaiger E

Labels: MiParea: Exercise physiology;nutrition;life style  Pathology: Aging;senescence 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex V;ATP synthase  Regulation: Substrate 

Pathway: N, NS 

MitoEAGLE BME 

MitoEAGLE V_O2max/BME data base

• Human vastus lateralis
• 12 males
• 24 years
• Range of differenct endurance activities
• h = 1.79 m
• m = 75 kg
• BME = 1.12
• BMI = 23.4 kg·m^-2
• V_O2max/BM = 50.0 mL·min^-1·kg^-1
• Isolated mitochondria; 25 °C; GS_P; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 120.6 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• 10.3 µM mt-protein/mg m_w

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• Human vastus lateralis
• 1 female & 10 males
• 72 years
• Range of differenct endurance activities
• h = 1.75 m
• m = 80 kg
• BME = 1.28
• BMI = 26.1 kg·m^-2
• V_O2max/BM = 34.0 mL·min^-1·kg^-1
• Isolated mitochondria; 25 °C; GS_P; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 115.1 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• 10.3 µM mt-protein/mg m_w

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