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Difference between revisions of "Respiratory acceptor control ratio"

From Bioblast
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|abbr=RCR
|abbr=RCR
|description=The '''respiratory acceptor control ratio''' (RCR) is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). RCR is directly but non-linearly related to the [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = 1-''L/P''. Whereas the normalized flux ratio ''j<sub>≈P</sub>'' has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses.  In intact cells, the term RCR has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the [[ETS coupling efficiency]], ''j<sub>≈E</sub>''= 1-''L/E'' [4].
|description=The '''respiratory acceptor control ratio''' (RCR) is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). RCR is directly but non-linearly related to the [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = 1-''L/P''. Whereas the normalized flux ratio ''j<sub>≈P</sub>'' has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses.  In intact cells, the term RCR has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the [[ETS coupling efficiency]], ''j<sub>≈E</sub>''= 1-''L/E'' [4].
|info=[[Chance 1955 J Biol Chem-III]], [[Gnaiger 2014 MitoPathways]]
|info=[[Chance 1955 J Biol Chem-I]], [[Gnaiger 2014 MitoPathways]]
|type=Respiration
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=Respiratory control ratio
|mitopedia concept=Respiratory control ratio
|type=Respiration
}}
}}
{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry
|mitopedia method=Respirometry
|type=Respiration
}}
}}
{{MitoPedia O2k and high-resolution respirometry|type=Respiration
{{MitoPedia O2k and high-resolution respirometry|type=Respiration
}}
{{MitoPedia topics|type=Respiration
}}
}}
== Compare ==
== Compare ==
* [[OXPHOS coupling efficiency]]
::::* [[OXPHOS coupling efficiency]]
* ''Discussion:'' OXPHOS versus [[ETS capacity]].
::::* ''Discussion:'' OXPHOS versus [[ETS capacity]].
* ''More details:'' »[[ETS coupling efficiency]]
::::* ''More details:'' »[[ETS coupling efficiency]]
* [[Talk:Respiratory acceptor control ratio |Discussion]]
::::* [[Talk:Respiratory acceptor control ratio |Discussion]]


== References ==
== References ==
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217:409-27. - [[Chance 1955 JBC-III |»Bioblast link«]]
 
# Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J|»Bioblast link«]]
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93. - [[Chance 1955 J Biol Chem-I |»Bioblast link«]] - "No extremely rapid phase of respiration is observed to precede the steady rate caused by the addition of phosphate acceptor; phosphate acceptor sets the respiratory system into operation very rapidly and at a relatively constant rate. The constant-respiration rate of the phosphorylating system is maintained until the concentration of phosphate acceptor has fallen to a low value; then the respiration abruptly falls by a factor of 4- to l0-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxidative phosphorylation system for ADP."
# Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - [[Gnaiger 2009 Int J Biochem Cell Biol|»Bioblast link«]]
:::# Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J|»Bioblast link«]]
# Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - [[Gnaiger 2014 MitoPathways |»Bioblast link«]]
:::# Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - [[Gnaiger 2009 Int J Biochem Cell Biol|»Bioblast link«]]
:::# Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - [[Gnaiger 2014 MitoPathways |»Bioblast link«]]

Revision as of 17:07, 9 October 2017


high-resolution terminology - matching measurements at high-resolution


Respiratory acceptor control ratio

Description

The respiratory acceptor control ratio (RCR) is defined as State 3/State 4 [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, L/P (when State 3 is equivalent to the OXPHOS state, P). RCR is directly but non-linearly related to the OXPHOS coupling efficiency, j≈P = 1-L/P. Whereas the normalized flux ratio j≈P has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, the term RCR has been used for the ratio State 3u/State 4o, i.e. for the inverse L/E ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the ETS coupling efficiency, j≈E= 1-L/E [4].

Abbreviation: RCR

Reference: Chance 1955 J Biol Chem-I, Gnaiger 2014 MitoPathways


MitoPedia concepts: Respiratory control ratio 


MitoPedia methods: Respirometry 



Compare

References

  1. Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93. - »Bioblast link« - "No extremely rapid phase of respiration is observed to precede the steady rate caused by the addition of phosphate acceptor; phosphate acceptor sets the respiratory system into operation very rapidly and at a relatively constant rate. The constant-respiration rate of the phosphorylating system is maintained until the concentration of phosphate acceptor has fallen to a low value; then the respiration abruptly falls by a factor of 4- to l0-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxidative phosphorylation system for ADP."
  2. Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
  3. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - »Bioblast link«
  4. Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - »Bioblast link«