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Difference between revisions of "Respiratory acceptor control ratio"

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{{MitoPedia
{{MitoPedia
|abbr=RCR
|abbr=''RCR''
|description=The '''respiratory control ratio''' (RCR) is defined as [[State 3]]/[[State 4]] ([[Chance 1955 JBC-III|Chance and Williams 1955]]). Considering an index of [[uncoupling]], RCR should be replaced by the ''[[L/E]]'' ratio, if ''[[P/E]]''<1.0 ([[Gnaiger_2009_IJBCB|Gnaiger 2009]]).  In intact cells, RCR has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''L/E'' ratio ([[Hutter_2004_BJ|Huetter et al., 2004]]).
|description=The '''respiratory acceptor control ratio''' (''RCR'') is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], ''RCR'' is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). ''RCR'' is directly but non-linearly related to the [[OXPHOS-coupling efficiency]], ''j<sub>≈P</sub>'' = 1-''L/P''. Whereas the normalized flux ratio ''j<sub>≈P</sub>'' has boundaries from 0.0 to 1.0, ''RCR'' ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses.  In intact cells, the term ''RCR'' has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, ''RCR'' should be replaced by the [[ET-coupling efficiency]], ''j<sub>≈E</sub>''= 1-''L/E'' [4].
|info=[[Chance 1955 JBC-III]]; [[MiPNet12.15]]
|info=[[Chance 1955 J Biol Chem-I]], [[Gnaiger 2020 MitoPathways]], [[BEC 2020.1]]
|type=Respiration
}}
}}
{{MitoPedia topics
== Compare ==
|mitopedia topic=Respiratory state
::::* [[OXPHOS-coupling efficiency]]
|type=Respiration
::::* ''Discussion:'' OXPHOS versus [[ET capacity]].
::::* ''More details:'' »[[ET-coupling efficiency]]
::::* [[Talk:Respiratory acceptor control ratio |Discussion]]
 
{{Keywords: Uncoupling}}
 
== References ==
 
:::# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93. - [[Chance 1955 J Biol Chem-I |»Bioblast link«]] - "No extremely rapid phase of respiration is observed to precede the steady rate caused by the addition of phosphate acceptor; phosphate acceptor sets the respiratory system into operation very rapidly and at a relatively constant rate. The constant-respiration rate of the phosphorylating system is maintained until the concentration of phosphate acceptor has fallen to a low value; then the respiration abruptly falls by a factor of 4- to l0-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxidative phosphorylation system for ADP."
:::# Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J|»Bioblast link«]]
:::# Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - [[Gnaiger 2009 Int J Biochem Cell Biol|»Bioblast link«]]
:::# Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - [[Gnaiger 2014 MitoPathways |»Bioblast link«]]
 
{{MitoPedia concepts
|mitopedia concept=Respiratory control ratio
}}
}}
The RCR is discussed in: [[Talk:ETS capacity]]
{{MitoPedia methods
|mitopedia method=Respirometry
}}
{{MitoPedia O2k and high-resolution respirometry|type=Respiration
}}
 
[[Category:Coupling control]]

Revision as of 13:58, 8 June 2020


high-resolution terminology - matching measurements at high-resolution


Respiratory acceptor control ratio

Description

The respiratory acceptor control ratio (RCR) is defined as State 3/State 4 [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, L/P (when State 3 is equivalent to the OXPHOS state, P). RCR is directly but non-linearly related to the OXPHOS-coupling efficiency, j≈P = 1-L/P. Whereas the normalized flux ratio j≈P has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, the term RCR has been used for the ratio State 3u/State 4o, i.e. for the inverse L/E ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the ET-coupling efficiency, j≈E= 1-L/E [4].

Abbreviation: RCR

Reference: Chance 1955 J Biol Chem-I, Gnaiger 2020 MitoPathways, BEC 2020.1

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Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Specific
» Artefacts by single dose uncoupling
» ATP synthase
» CCCP
» Coupling-control protocol
» DNP
» Dyscoupled respiration
» FCCP
» Is respiration uncoupled - noncoupled - dyscoupled?
» Noncoupled respiration: Discussion
» Uncoupler
» Uncoupled respiration - see » Noncoupled respiration
» Uncoupling proteins
» Uncoupling protein 1
» Uncoupler titrations - Optimum uncoupler concentration
Respiratory states and control ratios
» Biochemical coupling efficiency
» Coupling-control state
» Electron-transfer-pathway state
» Electron-transfer pathway
E.jpg ET capacity
» E-L coupling efficiency
» Flux control efficiency
» Flux control ratio
» LEAK-control ratio
» LEAK respiration
» Noncoupled respiration
» OXPHOS
» OXPHOS capacity; » State 3
» OXPHOS-control ratio, P/E ratio
» Respiratory acceptor control ratio
» ROUTINE-control ratio
» ROUTINE respiration
» ROUTINE state
» State 3u
» State 4
» Uncoupling-control ratio UCR
General (alphabetical order)
» Adenine nucleotide translocase
» Adenylates
» Electron transfer pathway
» Mitochondrial preparations
» mt-membrane potential
» Oxygen flux
» Phosphorylation system
» Proton leak
» Proton slip
» TIP2k
Other keyword lists
» Template:Keywords: Force and membrane potential


References

  1. Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93. - »Bioblast link« - "No extremely rapid phase of respiration is observed to precede the steady rate caused by the addition of phosphate acceptor; phosphate acceptor sets the respiratory system into operation very rapidly and at a relatively constant rate. The constant-respiration rate of the phosphorylating system is maintained until the concentration of phosphate acceptor has fallen to a low value; then the respiration abruptly falls by a factor of 4- to l0-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxidative phosphorylation system for ADP."
  2. Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
  3. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - »Bioblast link«
  4. Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - »Bioblast link«


MitoPedia concepts: Respiratory control ratio 


MitoPedia methods: Respirometry