Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Respiratory acceptor control ratio"

From Bioblast
Line 1: Line 1:
{{MitoPedia
{{MitoPedia
|abbr=RCR
|abbr=RCR
|description=The '''respiratory acceptor control ratio''' (RCR) is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). Considering an index of [[uncoupling]], RCR is equivalent to the inverse ''L/E'' ratio if ''P/E''=1.0, and should be replaced by the ''[[L/E]]'' ratio, if ''[[P/E]]''<1.0 [2]. In intact cells, RCR has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''L/E'' ratio [3].
|description=The '''respiratory acceptor control ratio''' (RCR) is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). RCR is directly but non-linearly related to the [[phosphorylation control factor]], ''≈P/P'' = 1-''L/P''. Whereas ''≈P/P'' has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, RCR has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the [[biochemical coupling efficiency]], ''j<sub>E-L</sub>''= 1-''L/E'' [4].  
|info=[[Chance 1955 JBC-III]], [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]
|info=[[Chance 1955 JBC-III]], [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]
|type=Respiration
|type=Respiration
Line 30: Line 30:
== References ==
== References ==
# [[Chance 1955 JBC-III|Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217: 409-427.]]
# [[Chance 1955 JBC-III|Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217: 409-427.]]
# [[Huetter_2004_Biochem J|Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]]
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]
# [[Huetter_2004_Biochem J|Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]]
# [[Gnaiger 2014 MitoPathways |Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck]]

Revision as of 16:13, 9 August 2014


high-resolution terminology - matching measurements at high-resolution


Respiratory acceptor control ratio

Description

The respiratory acceptor control ratio (RCR) is defined as State 3/State 4 [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, L/P (when State 3 is equivalent to the OXPHOS state, P). RCR is directly but non-linearly related to the phosphorylation control factor, ≈P/P = 1-L/P. Whereas ≈P/P has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, RCR has been used for the ratio State 3u/State 4o, i.e. for the inverse L/E ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the biochemical coupling efficiency, jE-L= 1-L/E [4].

Abbreviation: RCR

Reference: Chance 1955 JBC-III, Gnaiger 2012 MitoPathways


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

Discussion

The RCR is discussed in the context of OXPHOS versus ETS capacity.


Problem: The RCR is low in isolated mitochondria

At a high mitochondrial concentration (0.5 mg protein/ml), ADP is exhausted very rapidly if added at a concentration typically used for State 3 measurements (200-300 µM), which may stimulate flux below the ADP-saturated state of OXPHOS capacity. The slope increases sharply and returns to State 4 (LEAK), even before the plot of flux shows the true maximum, resulting in a low apparent RCR.

Tests and Solutions

  1. Reduce the data recording interval (standard is 2 seconds) to the minimum of 0.2 s (setting in the Oxygraph Control window; see MiPNet12.06 O2k-Start). As the data recording interval is reduced, the flux appears more noisy, but represents transitions more accurately and reduces the apparent time-delay. At high flux per volume, the increased noise level presents no problem. Flux then shows a period of constant OXPHOS capacity rather than a sharp peak.
  2. Reduce the mitochondrial concentration, thus prolonging the duration of ADP-saturated respiration (OXPHOS capacity) over 120 seconds. If flux reaches a constant maximum value, then analysis is possible without application of signal deconvolution (standard plot of flux in DatLab).
  3. Otherwise, export the data into DatLab 2, and apply a time correction (signal deconvolution), as described by Gnaiger 2001 Respir Physiol.
  4. See also ‘Notes on Time resolution’.

References

  1. Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217: 409-427.
  2. Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.
  3. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.
  4. Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck