https://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&feed=atom&action=historyRun DL-Protocol/Set O2 limit - Revision history2024-03-28T16:37:25ZRevision history for this page on the wikiMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=234797&oldid=prevCecatto Cristiane at 10:45, 12 December 20222022-12-12T10:45:49Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::'''The DL-Protocol window''' appears on the right side for every chamber. It consists of three buttons at the top, a name field, and a list to display events and marks.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::'''The DL-Protocol window''' appears on the right side for every chamber. It consists of three buttons at the top, a name field, and a list to display events and marks.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Buttons'''. The [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button is used to hide/show the DL-Protocol window (similar to 'Show DL-Protocol' in the 'Protocol' menu). Clicking the [[File:Hide_marks.png|x17px]]/[[File:Show_marks.png|x17px]] button toggles between hiding or showing the marks in the list. When the marks in the DL-Protocol are hidden the user only needs to focus on the sequence of events, facilitating the experimental procedure. The <del style="font-weight: bold; text-decoration: none;">[[File:Edit.png|60px]] </del>button opens the <del style="font-weight: bold; text-decoration: none;">[[#</del>DL-<del style="font-weight: bold; text-decoration: none;">Protocols-anchor2|'</del>Edit <del style="font-weight: bold; text-decoration: none;">DL-Protocol' </del>window<del style="font-weight: bold; text-decoration: none;">]] </del>to make changes in events and marks in the DL-Protocol. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Buttons'''. The [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button is used to hide/show the DL-Protocol window (similar to 'Show DL-Protocol' in the 'Protocol' menu). Clicking the [[File:Hide_marks.png|x17px]]/[[File:Show_marks.png|x17px]] button toggles between hiding or showing the marks in the list. When the marks in the DL-Protocol are hidden the user only needs to focus on the sequence of events, facilitating the experimental procedure. The <ins style="font-weight: bold; text-decoration: none;">"Overview" </ins>button opens the <ins style="font-weight: bold; text-decoration: none;">"</ins>DL-<ins style="font-weight: bold; text-decoration: none;">Protocol: Overview / </ins>Edit <ins style="font-weight: bold; text-decoration: none;">concentrations" </ins>window to make changes in events and marks in the DL-Protocol. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Information'''. Below the buttons, the 'name field' automatically displays the name of the loaded protocol.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Information'''. Below the buttons, the 'name field' automatically displays the name of the loaded protocol.</div></td></tr>
</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=232549&oldid=prevCecatto Cristiane at 12:58, 28 September 20222022-09-28T12:58:13Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia without banner</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]]<del style="font-weight: bold; text-decoration: none;">s </del>provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]] <ins style="font-weight: bold; text-decoration: none;">(SUIT) </ins>provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__TOC__</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__TOC__</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Events and marks basics'''.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Events and marks basics'''.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::**The [[#DL-Protocol window|'DL-Protocol window']] shows the sequence of '''DL-Protocol events''' and '''DL-Protocol marks''' that have to be set to complete an experiment. It is also possible to set '''user-defined marks''' and '''user-defined events''' (menu 'Experiment' -> 'Add event' or F4 or <del style="font-weight: bold; text-decoration: none;">STRG</del>-left-click in plot), which will not affect the DL-Protocol and will be stored together with the DL-Protocol marks and events in the .DLD data file. Extra '''user-defined events''' are of importance for unpredictable occurrences, e.g. "Open" and "Close" events, when a chamber needs to be re-oxygenized. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::**The [[#DL-Protocol window|'DL-Protocol window']] shows the sequence of '''DL-Protocol events''' and '''DL-Protocol marks''' that have to be set to complete an experiment. It is also possible to set '''user-defined marks''' and '''user-defined events''' (menu 'Experiment' -> 'Add event' or F4 or <ins style="font-weight: bold; text-decoration: none;">Ctrl</ins>-left-click in plot), which will not affect the DL-Protocol and will be stored together with the DL-Protocol marks and events in the .DLD data file. Extra '''user-defined events''' are of importance for unpredictable occurrences, e.g. "Open" and "Close" events, when a chamber needs to be re-oxygenized. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**It is possible to edit/change a '''user-defined mark''' or '''user-defined event''' into '''DL-Protocol mark''' or '''DL-Protocol event''', respectively.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**It is possible to edit/change a '''user-defined mark''' or '''user-defined event''' into '''DL-Protocol mark''' or '''DL-Protocol event''', respectively.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**In most instances, a '''DL-Protocol event''' is a titration step of a specific substance according to the DL Protocol. Thereby, the DL-Protocol event demarcates a specific 'state' (in particular a [[MitoPedia:_Respiratory_states|respiratory state]], depending on the titrated substance) until the next DL-Protocol event. Within a 'state' a DL-Protocol mark is set to read out the state-specific [[Oxygen_flux|oxygen flux]] (or any other signal such as oxygen or fluorescence - must be defined in the DL-Protocol).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**In most instances, a '''DL-Protocol event''' is a titration step of a specific substance according to the DL Protocol. Thereby, the DL-Protocol event demarcates a specific 'state' (in particular a [[MitoPedia:_Respiratory_states|respiratory state]], depending on the titrated substance) until the next DL-Protocol event. Within a 'state' a DL-Protocol mark is set to read out the state-specific [[Oxygen_flux|oxygen flux]] (or any other signal such as oxygen or fluorescence - must be defined in the DL-Protocol).</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Please note: White and yellow color-coded events are not yet set, yellow only indicates (logically) the next event in the DL-Protocol to be set. Thus, left clicking any white DL-Protocol event in the list will also open the 'Event information window' to set the event. However, setting the white DL-Protocol event will lead to a violation of the DL-Protocol sequence and this incorrect DL-Protocol event will appear in red. This problem is solved by editing the incorrectly set DL-Protocol event (right-click in the 'DL-Protocol window' list to open the 'Event information window' or left clicking in the plot) and deleting it from the plot. Then proceed with the yellow DL-Protocol event. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Please note: White and yellow color-coded events are not yet set, yellow only indicates (logically) the next event in the DL-Protocol to be set. Thus, left clicking any white DL-Protocol event in the list will also open the 'Event information window' to set the event. However, setting the white DL-Protocol event will lead to a violation of the DL-Protocol sequence and this incorrect DL-Protocol event will appear in red. This problem is solved by editing the incorrectly set DL-Protocol event (right-click in the 'DL-Protocol window' list to open the 'Event information window' or left clicking in the plot) and deleting it from the plot. Then proceed with the yellow DL-Protocol event. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**When a multi-event is encountered to be set for the first time, it appears in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>). After that it appears in aqua (<span style="background-color:#00FFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>) and can be set multiple times, according to the experimental needs. Do not be confused that the next DL-Protocol event in the list (after the multi-event) is already displayed in yellow!</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**When a multi-event is encountered to be set for the first time, it appears in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>). After that it appears in aqua (<span style="background-color:#00FFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>) and can be set multiple times, according to the experimental needs. Do not be confused that the next DL-Protocol event in the list (after the multi-event) is already displayed in yellow!</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::** User-defined events can be set during the run at any time point, e.g. for "O" (open) and "C" (close) events when re-oxygenation of the chamber is necessary. Therefore chose menu 'Experiment' -> 'Add event' (press F4) or <del style="font-weight: bold; text-decoration: none;">STRG</del>-left-click in plot. By default, user-defined events will always be named "Event" in the plot and will not appear in the DL-Protocol list. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::** User-defined events can be set during the run at any time point, e.g. for "O" (open) and "C" (close) events when re-oxygenation of the chamber is necessary. Therefore chose menu 'Experiment' -> 'Add event' (press F4) or <ins style="font-weight: bold; text-decoration: none;">Ctrl</ins>-left-click in plot. By default, user-defined events will always be named "Event" in the plot and will not appear in the DL-Protocol list. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** During the run it is also possible to set DL-Protocol marks or user-defined marks, e.g. to check the flux at a certain time-point/position. Keep in mind that only the marks for the active plot (O2 or O2 flux) are shown, DL-Protocol events are always displayed. In general, it is more convenient to set the DL-Protocol marks after the experimental run is completed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** During the run it is also possible to set DL-Protocol marks or user-defined marks, e.g. to check the flux at a certain time-point/position. Keep in mind that only the marks for the active plot (O2 or O2 flux) are shown, DL-Protocol events are always displayed. In general, it is more convenient to set the DL-Protocol marks after the experimental run is completed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** After the last DL-Protocol event has been set correctly, the DL-Protocol run is completed. Save the file (Menu 'File' -> 'Save and disconnect'), the O2k can now be switched off.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** After the last DL-Protocol event has been set correctly, the DL-Protocol run is completed. Save the file (Menu 'File' -> 'Save and disconnect'), the O2k can now be switched off.</div></td></tr>
</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=225538&oldid=prevSpitzer Gudrun: added technical support banner2022-03-21T11:25:21Z<p>added technical support banner</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:25, 21 March 2022</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|abbr=</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia <ins style="font-weight: bold; text-decoration: none;">without banner</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]]s provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]]s provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td></tr>
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</table>Spitzer Gudrunhttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=225415&oldid=prevCecatto Cristiane at 10:37, 15 March 20222022-03-15T10:37:08Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:37, 15 March 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the <del style="font-weight: bold; text-decoration: none;">according </del>chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit <del style="font-weight: bold; text-decoration: none;">breech</del>), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the <ins style="font-weight: bold; text-decoration: none;">accordingly </ins>chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit <ins style="font-weight: bold; text-decoration: none;">breach</ins>), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Show DL-Protocol ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Show DL-Protocol ===</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l59">Line 59:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol window ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol window ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:DL-Protocol window, different protocols.png|thumb|right|200px|link=http://wiki.oroboros.at/images/0/04/DL-Protocol_window.png| The '''DL-Protocol windows''' for chamber A (up) and chamber B (down) are shown with different DL-protocols as indicated by the DL-protocol name in the name field (1, red oval)]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:DL-Protocol window, different protocols.png|thumb|right|200px|link=http://wiki.oroboros.at/images/0/04/DL-Protocol_window.png| The '''DL-Protocol windows''' for chamber A (up) and chamber B (down) are shown with different DL-protocols as indicated by the DL-protocol name in the name field (1, red oval)]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::'''The DL-Protocol window''' appears <del style="font-weight: bold; text-decoration: none;">to </del>the <del style="font-weight: bold; text-decoration: none;">very </del>right for every chamber. It consists of three buttons at the top, a name field and a list to display events and marks.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::'''The DL-Protocol window''' appears <ins style="font-weight: bold; text-decoration: none;">on </ins>the right <ins style="font-weight: bold; text-decoration: none;">side </ins>for every chamber. It consists of three buttons at the top, a name field<ins style="font-weight: bold; text-decoration: none;">, </ins>and a list to display events and marks.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Buttons'''. The [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button is used to hide/show the DL-Protocol window (similar to 'Show DL-Protocol' in the 'Protocol' menu). Clicking the [[File:Hide_marks.png|x17px]]/[[File:Show_marks.png|x17px]] button toggles between hiding or showing the marks in the list. When the marks in the DL-Protocol are hidden the user only needs to focus on the sequence of events, facilitating the experimental procedure. The [[File:Edit.png|60px]] button opens the [[#DL-Protocols-anchor2|'Edit DL-Protocol' window]] to make changes in events and marks in the DL-Protocol. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::*'''Buttons'''. The [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button is used to hide/show the DL-Protocol window (similar to 'Show DL-Protocol' in the 'Protocol' menu). Clicking the [[File:Hide_marks.png|x17px]]/[[File:Show_marks.png|x17px]] button toggles between hiding or showing the marks in the list. When the marks in the DL-Protocol are hidden the user only needs to focus on the sequence of events, facilitating the experimental procedure. The [[File:Edit.png|60px]] button opens the [[#DL-Protocols-anchor2|'Edit DL-Protocol' window]] to make changes in events and marks in the DL-Protocol. </div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l100">Line 100:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''DL-Protocol description'''. This field contains general, DL-Protocol specific information and can be changed/edited by the user.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''DL-Protocol description'''. This field contains general, DL-Protocol specific information and can be changed/edited by the user.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::::*Changes can be accepted [[File:Save-button.png|x17px]] or rejected [[File:Chancel-button.png|x17px]]. Finally, changes in the DL-Protocol can be saved/exported to generate a user modified DL-Protocol (*.DLPU), see [[#DL-Protocols-anchor3|A: Export DL-Protocol]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::::*Changes can be accepted [[File:Save-button.png|x17px]] or rejected [[File:Chancel-button.png|x17px]]. Finally, changes in the DL-Protocol can be saved/exported to generate a user<ins style="font-weight: bold; text-decoration: none;">-</ins>modified DL-Protocol (*.DLPU), see [[#DL-Protocols-anchor3|A: Export DL-Protocol]].</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Considering the experimental timing (e.g. flux has stabilized), left-klick on the first '''DL Protocol event''' in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;E: 1xxx'''</span>) of the DL Protocol and the Event information window will open to set the DL-Protocol event in the plot. When the DL-Protocol event is set correctly according to the protocol sequence it turns into green (<span style="background-color:#377A34; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px; color:#FFFFFF">'''>>&nbsp;E: 1xxx'''</span>) in the DL-Protocol window list. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Considering the experimental timing (e.g. flux has stabilized), left-klick on the first '''DL Protocol event''' in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;E: 1xxx'''</span>) of the DL Protocol and the Event information window will open to set the DL-Protocol event in the plot. When the DL-Protocol event is set correctly according to the protocol sequence it turns into green (<span style="background-color:#377A34; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px; color:#FFFFFF">'''>>&nbsp;E: 1xxx'''</span>) in the DL-Protocol window list. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**Proceed with the next yellow DL-Protocol event <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;E: 2xxx'''</span> at the according time point (after flux has stabilized or experiment specific timing).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**Proceed with the next yellow DL-Protocol event <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;E: 2xxx'''</span> at the according time point (after flux has stabilized or experiment specific timing).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::** Please note: White and yellow color-coded events are not yet set, yellow only <del style="font-weight: bold; text-decoration: none;"> </del>indicates (logically) the next event in the DL-Protocol to be set. Thus, left clicking any white DL-Protocol event in the list will also open the 'Event information window' to set the event. However, setting the white DL-Protocol event will lead to a violation of the DL-Protocol sequence and this incorrect DL-Protocol event will appear in red. This problem is solved by editing the incorrectly set DL-Protocol event (right-click in the 'DL-Protocol window' list to open the 'Event information window' or left clicking in the plot) and deleting it from the plot. Then proceed with the yellow DL-Protocol event. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::** Please note: White and yellow color-coded events are not yet set, yellow only indicates (logically) the next event in the DL-Protocol to be set. Thus, left clicking any white DL-Protocol event in the list will also open the 'Event information window' to set the event. However, setting the white DL-Protocol event will lead to a violation of the DL-Protocol sequence and this incorrect DL-Protocol event will appear in red. This problem is solved by editing the incorrectly set DL-Protocol event (right-click in the 'DL-Protocol window' list to open the 'Event information window' or left clicking in the plot) and deleting it from the plot. Then proceed with the yellow DL-Protocol event. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**When a multi-event is encountered to be set for the first time, it appears in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>). After that it appears in aqua (<span style="background-color:#00FFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>) and can be set multiple times, according to the experimental needs. Do not be confused that the next DL-Protocol event in the list (after the multi-event) is already displayed in yellow!</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::**When a multi-event is encountered to be set for the first time, it appears in yellow (<span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>). After that it appears in aqua (<span style="background-color:#00FFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''<big>∗</big>&nbsp;E: xxx <big>*</big>'''</span>) and can be set multiple times, according to the experimental needs. Do not be confused that the next DL-Protocol event in the list (after the multi-event) is already displayed in yellow!</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::** User-defined events can be set during the run at any time point, e.g. for "O" (open) and "C" (close) events when re-oxygenation of the chamber is necessary. Therefore chose menu 'Experiment' -> 'Add event' (press F4) or STRG-left-click in plot. By default, <del style="font-weight: bold; text-decoration: none;">a </del>user-defined events will always be named "Event" in the plot and will not appear in the DL-Protocol list. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::** User-defined events can be set during the run at any time point, e.g. for "O" (open) and "C" (close) events when re-oxygenation of the chamber is necessary. Therefore chose menu 'Experiment' -> 'Add event' (press F4) or STRG-left-click in plot. By default, user-defined events will always be named "Event" in the plot and will not appear in the DL-Protocol list. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** During the run it is also possible to set DL-Protocol marks or user-defined marks, e.g. to check the flux at a certain time-point/position. Keep in mind that only the marks for the active plot (O2 or O2 flux) are shown, DL-Protocol events are always displayed. In general, it is more convenient to set the DL-Protocol marks after the experimental run is completed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** During the run it is also possible to set DL-Protocol marks or user-defined marks, e.g. to check the flux at a certain time-point/position. Keep in mind that only the marks for the active plot (O2 or O2 flux) are shown, DL-Protocol events are always displayed. In general, it is more convenient to set the DL-Protocol marks after the experimental run is completed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** After the last DL-Protocol event has been set correctly, the DL-Protocol run is completed. Save the file (Menu 'File' -> 'Save and disconnect'), the O2k can now be switched off.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** After the last DL-Protocol event has been set correctly, the DL-Protocol run is completed. Save the file (Menu 'File' -> 'Save and disconnect'), the O2k can now be switched off.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Open the .DLD file (Menu 'File' -> 'Open'; when DatLab is starting up, first choose a 'User' and then click 'Open DLD file' in the start-up window). Keep in mind, only the DL-Protocol marks for the active plot (O2 or O2 flux) are shown in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. DL-Protocol marks that have not yet been set appear in white (<span style="background-color:#FFFFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** Open the .DLD file (Menu 'File' -> 'Open'; when DatLab is starting up, first choose a 'User' and then click 'Open DLD file' in the start-up window). Keep in mind, only the DL-Protocol marks for the active plot (O2 or O2 flux) are shown in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. DL-Protocol marks that have not yet been set appear in white (<span style="background-color:#FFFFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** '''Set a mark''' by holding the 'Shift' key and demarcate the desired area in the plot with the mouse cursor while holding the '''left''' mouse button. When setting the mark in a specific state after the according DL-Protocol event, the mark will be named automatically (as defined in the DL-protocol) and is classified as a DL-Protocol mark. Unlike DL-Protocol events, the DL-Protocol marks can be set in any order. In the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] a correctly set DL-Protocol mark will appear in light green (<span style="background-color:#94FD94; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** '''Set a mark''' by holding the 'Shift' key and demarcate the desired area in the plot with the mouse cursor while holding the '''left''' mouse button. When setting the mark in a specific state after the according DL-Protocol event, the mark will be named automatically (as defined in the DL-protocol) and is classified as a DL-Protocol mark. Unlike DL-Protocol events, the DL-Protocol marks can be set in any order. In the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] a correctly set DL-Protocol mark will appear in light green (<span style="background-color:#94FD94; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::**When setting a second or any further mark in a state (behind a DL-Protocol event), this mark will be recognized as a user-defined mark, automatically named with a single letter (alphabetically, starting with 'a'). A user defined mark will not show up in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::**When setting a second or any further mark in a state (behind a DL-Protocol event), this mark will be recognized as a user-defined mark, automatically named with a single letter (alphabetically, starting with 'a'). A user<ins style="font-weight: bold; text-decoration: none;">-</ins>defined mark will not show up in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** To '''remove a mark''' from a plot, hold the 'Shift' key and completely demarcate the mark-area with the mouse cursor while holding the '''right''' mouse button. When a DL-Protocol mark is removed from the plot its appearance in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] is reverted to white (<span style="background-color:#FFFFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** To '''remove a mark''' from a plot, hold the 'Shift' key and completely demarcate the mark-area with the mouse cursor while holding the '''right''' mouse button. When a DL-Protocol mark is removed from the plot its appearance in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] is reverted to white (<span style="background-color:#FFFFFF; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''>>&nbsp;M: xxx'''</span>).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** To '''edit a mark''', left-click the according mark-bar at the top in the plot-window or right-click the DL-Protocol mark (light green or red) in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. The [[#DL-Protocols-anchor11|'Mark information window' (for editing)]] appears and allows (i) editing the parameters 'Name', 'State', 'Final conc.[mM]', 'Titration vol. [µL]'or 'Comment', (ii) setting 'Outlier index threshold', (iii) checking 'Delete point', 'Interpolate points' or 'Recalc. slope' or (iv) selecting a different DL-Protocol mark from the list(double-left-clicking). Finally, confirm the changes with the 'OK' button.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::** To '''edit a mark''', left-click the according mark-bar at the top in the plot-window or right-click the DL-Protocol mark (light green or red) in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. The [[#DL-Protocols-anchor11|'Mark information window' (for editing)]] appears and allows (i) editing the parameters 'Name', 'State', 'Final conc.[mM]', 'Titration vol. [µL]'or 'Comment', (ii) setting 'Outlier index threshold', (iii) checking 'Delete point', 'Interpolate points' or 'Recalc. slope' or (iv) selecting a different DL-Protocol mark from the list(double-left-clicking). Finally, confirm the changes with the 'OK' button.</div></td></tr>
</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216768&oldid=prevCecatto Cristiane at 11:07, 2 April 20212021-04-02T11:07:46Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:07, 2 April 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{MitoPedia</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abbr=</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abbr=</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]]s provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [<del style="font-weight: bold; text-decoration: none;">https://www.bioblast.at/index.php/</del>MitoPedia:_SUIT library] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|description='''DL-Protocols''' (DLP) can be selected in DatLab 7 in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |Events]] and [[Marks - DatLab |Marks]] and can be assigned to O2k-Chamber A or B, or both. Linked to DL-Protocols are templates for storing exported data in a database and for data analysis. Instrumental DL-Protocols are used for calibrations and instrumental quality control, without experimental sample in the incubation medium. DL-Protocols for [[substrate-uncoupler-inhibitor titration]]s provide a guide through a sequence of [[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [<ins style="font-weight: bold; text-decoration: none;">[</ins>MitoPedia:_SUIT<ins style="font-weight: bold; text-decoration: none;">|</ins>library<ins style="font-weight: bold; text-decoration: none;">]</ins>] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k]] can be programmed for automatic control of titration steps in a DL-Protocol. In DatLab 7.4, it is possible to edit a DL-Protocol and save it as a [[Export_DL-Protocol_User_(*.DLPU)| user-specific DL-Protocol]] (*.DLPU). For more information, see: [[Enable DL-Protocol editing]]. A '''Lower O2 limit [µM]''' can be defined for each chamber, to trigger an automatic warning when the experimental O<sub>2</sub> concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__TOC__</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__TOC__</div></td></tr>
</table>Cecatto Cristianehttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216717&oldid=prevGnaiger Erich at 07:08, 31 March 20212021-03-31T07:08:47Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:08, 31 March 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Protocol menu ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Protocol menu ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Protocol-menu.png|thumb|right|200px|link=http://www.bioblast.at/images/8/8e/Protocol-menu.png|'''Protocol menu''']]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Protocol-menu.png|thumb|right|200px|link=http://www.bioblast.at/images/8/8e/Protocol-menu.png|'''Protocol menu''']]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::'''Show DL-Protocol''' can be check-marked ([[File:Check-mark.png|17px]]) to hide or show the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. Alternatively, the [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button (upper left) in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] can be used to hide or show the window. When DatLab is started for data recording, an empty [[DL-Protocols#DL-Protocol window|DL-Protocol window]] appears because no DL-protocol has been specified at this moment.</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"><div id="DL-Protocols-anchor4"></div></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::'''Synchronous DL-Protocol events'''. This option is only available when the same DL-Protocol is selected (in [[#DL-Protocols-anchor1|'Set DL-Protocol / O2 limit']]) for chamber A and B (protocol names are displayed in the name field of the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]). When checked [[File:Check-mark.png|17px]], the user only needs to follow one protocol sequence of either chamber A or B. For example, when an event is set for chamber A, it will also be automatically set for chamber B - the 'Event Information' window indicates "Set event in both chambers" in bold letters. </del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[File:Set_DL-_Protocol_O2_-_window.png|thumb|right|200px|link=http://wiki.oroboros.at/images/b/bf/Set_DL-_Protocol_O2_-_window.png|'''Set DL-Protocol / O2 limit'''. For chamber A (upper frame) a specific protocol is selected, showing the name ("SUIT-1_02_pce_D03"), the hyperlink to the Biobast in blue and additional information. Furthermore, the 'Lower O2 limit [µM]' alert is checked. In chamber B (lower frame) no DL-protocol is selected.]]</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><div id="DL-Protocols-anchor1"></div></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><div id="DL-Protocols-anchor1"></div></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::'''</del>Run DL-Protocol / Set O2 limit<del style="font-weight: bold; text-decoration: none;">'''. </del>In the 'Run DL-Protocol / Set O2 limit' window a DL-Protocol can be assigned to specific chamber.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== </ins>Run DL-Protocol / Set O2 limit <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: </ins>In the 'Run DL-Protocol / Set O2 limit' window a DL-Protocol can be assigned to specific chamber.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''[[File:SUIT button.png|50 px]]''' and '''[[File:Instrumental button.png|50 px]]''' buttons: specific SUIT protocols or Instrumental DL-protocols (either a *.DLP or *.DLPU file) can be selected for chamber A and B. '.DLP' is the original file format for DL-Protocols provided by Oroboros, '.DLPU' files are user-modified versions (see [[#DL-Protocols-anchor3|A: Export DL-Protocol]] and [[#DL-Protocols-anchor2|'Edit DL-Protocol' window]]) of a specific DL-Protocols ('*.DLP' file). </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''[[File:SUIT button.png|50 px]]''' and '''[[File:Instrumental button.png|50 px]]''' buttons: specific SUIT protocols or Instrumental DL-protocols (either a *.DLP or *.DLPU file) can be selected for chamber A and B. '.DLP' is the original file format for DL-Protocols provided by Oroboros, '.DLPU' files are user-modified versions (see [[#DL-Protocols-anchor3|A: Export DL-Protocol]] and [[#DL-Protocols-anchor2|'Edit DL-Protocol' window]]) of a specific DL-Protocols ('*.DLP' file). </div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l27">Line 27:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Instrumental: Browse DL-Protocols and templates<del style="font-weight: bold; text-decoration: none;">'''</del>: Opens the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Show DL-Protocol ===</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::: '''</del>SUIT: Browse DL-Protocols and templates<del style="font-weight: bold; text-decoration: none;">'''</del>: Opens the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: can be check-marked ([[File:Check-mark.png|17px]]) to hide or show the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]. Alternatively, the [[File:_Button_show-hide_DL-Protocol_window.png|17px]] button (upper left) in the [[DL-Protocols#DL-Protocol window|DL-Protocol window]] can be used to hide or show the window. When DatLab is started for data recording, an empty [[DL-Protocols#DL-Protocol window|DL-Protocol window]] appears because no DL-protocol has been specified at this moment.</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::: '''</del>Install Oroboros protocol packages<del style="font-weight: bold; text-decoration: none;">'''</del>: Enables installation of new SUIT protocol packages.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::: '''</del>Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT<del style="font-weight: bold; text-decoration: none;">'''</del>: Opens the library of SUIT protocols.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::'''[[Enable DL-Protocol editing]]'''</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><div id="DL-Protocols-anchor4"></div></ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Synchronous DL-Protocol events ===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: <ins style="font-weight: bold; text-decoration: none;">This option can be check-marked when the same DL-Protocol is selected (in [[#DL-Protocols-anchor1|'Set DL-Protocol / O2 limit']]) for chamber A and B (protocol names are displayed in the name field of the [[DL-Protocols#DL-Protocol window|DL-Protocol window]]). When checked [[File:Check-mark.png|17px]], the user only needs to follow one protocol sequence of either chamber A or B. For example, when an event is set for chamber A, it will also be automatically set for chamber B - the 'Event Information' window indicates "Set event in both chambers" in bold letters. </ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[File:Set_DL-_Protocol_O2_-_window.png|thumb|right|200px|link=http://wiki.oroboros.at/images/b/bf/Set_DL-_Protocol_O2_-_window.png|</ins>'''<ins style="font-weight: bold; text-decoration: none;">Set DL-Protocol / O2 limit'''. For chamber A (upper frame) a specific protocol is selected, showing the name ("SUIT-1_02_pce_D03"), the hyperlink to the Biobast in blue and additional information. Furthermore, the 'Lower O2 limit [µM]' alert is checked. In chamber B (lower frame) no DL-protocol is selected.]]</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== </ins>Instrumental: Browse DL-Protocols and templates <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::</ins>: Opens the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== </ins>SUIT: Browse DL-Protocols and templates <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::</ins>: Opens the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== </ins>Install Oroboros protocol packages <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::</ins>: Enables installation of new SUIT protocol packages.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== </ins>Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::</ins>: Opens the library of SUIT protocols.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Enable DL-Protocol editing ===</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:</ins>::: <ins style="font-weight: bold; text-decoration: none;">Can be check-marked. - ''Further details''</ins>: '''[[Enable DL-Protocol editing]]'''</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216667&oldid=prevTindle Lisa at 14:47, 29 March 20212021-03-29T14:47:37Z<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:47, 29 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l27">Line 27:</td>
<td colspan="2" class="diff-lineno">Line 27:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), click the red flashing "WARNING" button. A 'Warning window' then opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Instrumental: Browse DL-Protocols and templates''': <del style="font-weight: bold; text-decoration: none;">It opens </del>the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Instrumental: Browse DL-Protocols and templates''': <ins style="font-weight: bold; text-decoration: none;">Opens </ins>the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''SUIT: Browse DL-Protocols and templates''': <del style="font-weight: bold; text-decoration: none;">It opens </del>the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''SUIT: Browse DL-Protocols and templates''': <ins style="font-weight: bold; text-decoration: none;">Opens </ins>the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Install Oroboros protocol packages''': <del style="font-weight: bold; text-decoration: none;">It allows to install </del>of new packages <del style="font-weight: bold; text-decoration: none;">of SUIT protocols</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Install Oroboros protocol packages''': <ins style="font-weight: bold; text-decoration: none;">Enables installation </ins>of new <ins style="font-weight: bold; text-decoration: none;">SUIT protocol </ins>packages.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT''': <del style="font-weight: bold; text-decoration: none;">It opens </del>the library of SUIT protocols.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT''': <ins style="font-weight: bold; text-decoration: none;">Opens </ins>the library of SUIT protocols.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::'''[[Enable DL-Protocol editing]]'''</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::'''[[Enable DL-Protocol editing]]'''</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td></tr>
</table>Tindle Lisahttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216666&oldid=prevTindle Lisa at 14:41, 29 March 20212021-03-29T14:41:02Z<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:41, 29 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l25">Line 25:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), the red flashing "WARNING" button <del style="font-weight: bold; text-decoration: none;">has to be clicked</del>. <del style="font-weight: bold; text-decoration: none;">First, a </del>'Warning window' opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), <ins style="font-weight: bold; text-decoration: none;">click </ins>the red flashing "WARNING" button. <ins style="font-weight: bold; text-decoration: none;">A </ins>'Warning window' <ins style="font-weight: bold; text-decoration: none;">then </ins>opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Instrumental: Browse DL-Protocols and templates''': It opens the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Instrumental: Browse DL-Protocols and templates''': It opens the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''SUIT: Browse DL-Protocols and templates''': It opens the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''SUIT: Browse DL-Protocols and templates''': It opens the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</div></td></tr>
</table>Tindle Lisahttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216637&oldid=prevKomlodi Timea at 13:02, 29 March 20212021-03-29T13:02:46Z<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:02, 29 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l30">Line 30:</td>
<td colspan="2" class="diff-lineno">Line 30:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Install Oroboros protocol packages''': It allows to install of new packages of SUIT protocols.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Install Oroboros protocol packages''': It allows to install of new packages of SUIT protocols.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT''': It opens the library of SUIT protocols.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: '''Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT''': It opens the library of SUIT protocols.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: '''[[Enable DL-Protocol editing]]'''</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::'''[[Enable DL-Protocol editing]]'''</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>Komlodi Timeahttps://wiki.oroboros.at/index.php?title=Run_DL-Protocol/Set_O2_limit&diff=216636&oldid=prevKomlodi Timea at 13:02, 29 March 20212021-03-29T13:02:11Z<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
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<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:02, 29 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l26">Line 26:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Warning-O2.png|thumb|right|200px|link=http://wiki.oroboros.at/images/e/e0/Warning-O2.png|'''O2-limit warning button''']]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), the red flashing "WARNING" button has to be clicked. First, a 'Warning window' opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::*'''Lower O2 limit [µM]'''. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to enable notification of the next lower O<sub>2</sub> limit breech), the red flashing "WARNING" button has to be clicked. First, a 'Warning window' opens to show all warnings during the experiment. After closing the window, the reset is completed.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: '''Instrumental: Browse DL-Protocols and templates''': It opens the folders of instrumental protocols included in the DL protocol package with the corresponding excel analysis templates.</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: '''SUIT: Browse DL-Protocols and templates''': It opens the folders of SUIT protocols included in the DL protocol package with the corresponding excel analysis templates.</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: '''Install Oroboros protocol packages''': It allows to install of new packages of SUIT protocols.</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: '''Browse http://www.bioblast.at/index.php/MitoPedia:_SUIT''': It opens the library of SUIT protocols.</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: '''[[Enable DL-Protocol editing]]'''</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== DL-Protocol export == </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>Komlodi Timea