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SF6847

From Bioblast


high-resolution terminology - matching measurements at high-resolution


SF6847

Description

SF 6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.

Abbreviation: SF6847

Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta


MitoPedia topics: Uncoupler 


Application in HRR

Preparation

SF 6847/tyrphostin A9/malonoben (C18H22N2O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: FW = 282.38. Alternative source: Enzo, BML-EI215.
Caution: Toxic!
Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):