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SF6847

From Bioblast


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SF6847

Description

SF6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF6847 concentrations must be titrated carefully to evaluate the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.

Abbreviation: SF6847

Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta

Application in HRR

Preparation

SF 6847/tyrphostin A9/malonoben (C18H22N2O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: M = 282.38 gยทmol-1. Alternative source: Enzo, BML-EI215.
Caution: Toxic!
Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):
  1. With 5 mg in a dark glass vial: dissolve with 354.1 ยตL ethanol.
  2. Store in glass vials at -20 ยฐC.
Preparation of 500 ยตM stock solution (dissolved in 99.9% ethanol):
  1. Add 990 ยตL ethanol and 10 ยตL of the 50 mM stock solution to a dark glass vial.
  2. Store in glass vials at -20 ยฐC.
Preparation of 50 ยตM stock solution (dissolved in 99.9% ethanol):
  1. Add 900 ยตL ethanol and 100 ยตL of the 500 ยตM stock solution to a dark glass vial.
  2. Divide into 0.1 ml aliquotes, in dark glass vials.
  3. Store at -20 ยฐC.
O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 1 ยตl steps 50 ยตM stock solution using a 10 ยตL syringe (2 ml O2k-Chamber).
  • Final concentration: 25 nM steps.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


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Specific
ยป Artefacts by single dose uncoupling
ยป ATP synthase
ยป CCCP
ยป Coupling-control protocol
ยป DNP
ยป Dyscoupled respiration
ยป FCCP
ยป Is respiration uncoupled - noncoupled - dyscoupled?
ยป Noncoupled respiration: Discussion
ยป Uncoupler
ยป Uncoupled respiration - see ยป Noncoupled respiration
ยป Uncoupling proteins
ยป Uncoupling protein 1
ยป Uncoupler titrations - Optimum uncoupler concentration
Respiratory states and control ratios
ยป Biochemical coupling efficiency
ยป Coupling-control state
ยป Electron-transfer-pathway state
ยป Electron-transfer pathway
E.jpg ET capacity
ยป E-L coupling efficiency
ยป Flux control efficiency
ยป Flux control ratio
ยป LEAK-control ratio
ยป LEAK respiration
ยป Noncoupled respiration
ยป OXPHOS
ยป OXPHOS capacity; ยป State 3
ยป OXPHOS-control ratio, P/E ratio
ยป Respiratory acceptor control ratio
ยป ROUTINE-control ratio
ยป ROUTINE respiration
ยป ROUTINE state
ยป State 3u
ยป State 4
ยป Uncoupling-control ratio UCR
General (alphabetical order)
ยป Adenine nucleotide translocase
ยป Adenylates
ยป Electron transfer pathway
ยป Mitochondrial preparations
ยป mt-membrane potential
ยป Oxygen flux
ยป Phosphorylation system
ยป Proton leak
ยป Proton slip
ยป TIP2k
Other keyword lists
ยป Template:Keywords: Force and membrane potential



MitoPedia topics: Uncoupler