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Difference between revisions of "SUIT-002 O2 ce-pce D007a"

From Bioblast
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|abbr=FNSGp(PGM)
|abbr=FNSGp(PGM)
|description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]]
|description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]]
|SUIT number=D07a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
|SUIT number=D07a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd Β 
|info='''A: SUIT 2 is the RP2''' - [[MiPNet21.06 SUIT RP]]
|info='''A: SUIT RP2 specific for PBMC and platelets cells''' - [[MiPNet21.06 SUIT RP]]
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts

Revision as of 13:11, 22 August 2018


high-resolution terminology - matching measurements at high-resolution


SUIT-002 O2 ce-pce D007a

Description

1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png

Abbreviation: FNSGp(PGM)

Reference: A: SUIT RP2 specific for PBMC and platelets cells - MiPNet21.06 SUIT RP

SUIT number: D07a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

O2k-Application: O2


MitoPedia concepts: SUIT protocol, SUIT A 

MitoPedia: SUIT - SUIT reference protocol RP2
SUIT-category: FNSGp(PGM)
SUIT protocol pattern: diametral



Harmonized SUIT protocols

RP1 and RP2

SUIT RP1 SUIT-RP1 SUIT RP2 SUIT-RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

RP1&RP2.png Harmonization between RP1 and RP2

RP1 and RP2 in cells

RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.

SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Reference protocol RP1&RP2 pce.png Harmonization between RP1 and RP2 in cells


RP1 and RP2 in PBMCs and PTLs

In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs

SUIT RP1 in PBMCs and PTLsSUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
SUIT RP2 PBMCs and PLTs: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Reference protocol RP1&RP2 pce PBMCs PTLs.png Harmonization between RP1 and RP2 in PBMC and PTL cells


References