- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce-pce D020
Description
Abbreviation: ceP,S
Reference: A SUIT-003 Coupling control protocol and respirometric cell viability test (CCV protocol)
SUIT number: D020_ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;1Dig;1c;2Ama;3AsTm;4Azd
O2k-Application: O2
- SUIT-category: ceP,S
- SUIT protocol pattern: complex cell coupling control protocol ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;1Dig;1c;2Ama;3AsTm;4Azd
Steps and respiratory states
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
ce1
|
ROUTINE
|
|
|
ce1
- ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
|
ce1P
|
ROUTINE
|
|
|
ce1;ce1P
- ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
|
ce2Omy
|
L(Omy)
|
|
|
ce1;ce1P;ce2Omy
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
|
ce3U
|
E
|
|
|
ce1;ce1P;ce2Omy;ce3U
|
ce4Rot
|
ROX
|
|
|
ce1;ce1P;ce2Omy;ce3U;ce4Rot
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
|
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
ce5S
|
|
|
|
ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S
|
1Dig*
|
SE
|
S
|
CII
|
ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig
|
1c
|
SE
|
S
|
CII
|
ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c
- Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
- Noncoupled electron transfer state, ET state, with ET capacity E.
|
2Ama
|
ROX
|
|
|
ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
Step
|
Respiratory state
|
Pathway control
|
ET-Complex
|
Comment
|
## AsTm
|
AsTmE
|
CIV
|
CIV
|
|
## Azd
|
CHB
|
|
|
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- » Coupling control state
- » ET capacity
- » OXPHOS capacity
- » LEAK respiration
- Pathway control
- » Electron transfer pathway
- » Fatty acid oxidation pathway control state, F
- » NADH electron transfer-pathway state, N
- » Succinate pathway control state, S
- » NS-pathway control state, NS
- » Glycerophosphate pathway control state, Gp
- » Complex IV single step, CIV
- » Anaplerotic pathway control state
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Glossary
- » List of SUIT states
- » SUIT concept
Compare SUIT protocols
MitoPedia concepts:
SUIT protocol,
SUIT A,
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MitoPedia methods:
Respirometry