Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "SUIT-003 O2 ce D009"

From Bioblast
Line 12: Line 12:


== References ==
== References ==
{{#ask:[[Category:Publications]] [[Additional label:SUIT-003 O2 ce D009]]
{{#ask:[[Category:Publications]] [[Additional label::SUIT-003 O2 ce D009]]
|?Was published in year=Year
|?Was published in year=Year
|?Has title=Reference
|?Has title=Reference
Line 23: Line 23:
|order=descending
|order=descending
}}
}}


== ''R'';''L'';''E'' format_ROX ==
== ''R'';''L'';''E'' format_ROX ==

Revision as of 09:37, 21 January 2019


high-resolution terminology - matching measurements at high-resolution


SUIT-003 O2 ce D009

Description

Ce1;ce2Omy;ce3U-.png

Abbreviation: ce

Reference: A Huetter 2004 Biochem J - SUIT-003

SUIT number: D009_ce1;ce2Omy;ce3U;ce4Rot;ce5Ama

O2k-Application: O2

SUIT-category: ce
SUIT protocol pattern: linear cell (ce) coupling control protocol 1ce;2ceOmy;3ceU

Template:SUIT-003 O2 O2 ce D009

References

 YearReferenceOrganismTissue;cell
MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
HumanBlood cells
Lymphocyte
Gnaiger 2008 POS2008Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.MouseBlood cells
Huetter 2004 Biochem J2004Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. https://doi.org/10.1042/BJ20040095HumanFibroblast


R;L;E format_ROX

R;L;E format ROX.jpg
  • The ceCCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
  • Rot and Ama
In this ceCCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma).
Ce1;ce2Omy;ce3U;ce4Rot;ce5Ama.png

ce1;ce2Omy;ce3U;ce4Rot;ce5Ama

  • ce1;ce2Omy;ce3U;ce4Rot;ce5Ama
In this ceCCP, ROX is measured after stepwise titration of rotenone (ce4Rot) and antimycin A (ce5Ama). In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of animycin A (ce5Ama).
ceCCP (fibroblasts)


Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
  • 1ce: ROUTINE (R; routine state in cell-culture medium) in intact cells (ce);
  • 2ceOmy: LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin);
  • 3ceU: ET-pathway (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration);
  • 4ceRot: ROX (rotenone, Rot; residual oxygen consumption).
  • 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
  • Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«


MitoPedia concepts: SUIT protocol, SUIT A, Find 


MitoPedia methods: Respirometry