Difference between revisions of "SUIT-010"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=S | |abbr=ceS,S | ||
|description=[[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] | |description=[[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] | ||
|info='''A''' | |info='''A''' Optimum [[digitonin]] concentration for [[permeabilized cells |pce]] [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-010.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-010.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' S | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS,S | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ||
__TOC__ | __TOC__ | ||
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{{Template:SUIT-010}} | {{Template:SUIT-010}} | ||
== Strengths and limitations == | |||
== Compare SUIT protocols == | |||
::::* [[Ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama]] | |||
::::* [[SUIT-003]] | |||
::::* [[SUIT-006]] | |||
{{MitoPedia concepts | {{MitoPedia concepts |
Revision as of 09:25, 15 January 2019
Description
Abbreviation: ceS,S
Reference: A Optimum digitonin concentration for pce »Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
DLP applications
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
Compare SUIT protocols
MitoPedia concepts:
SUIT A
MitoPedia methods:
Respirometry