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SUIT-010

From Bioblast
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high-resolution terminology - matching measurements at high-resolution


SUIT-010

Description

Respirometric test of optimum digitonin concentration

Abbreviation: ceS,S

Reference: A Optimum digitonin concentration for pce Bioblast pdf »Versions

SUIT-category: ceS,S
SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c

DLP applications


References

 YearReferenceOrganismTissue;cell
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3Human
Mouse
Rat
Saccharomyces cerevisiae
Heart
Skeletal muscle
Endothelial;epithelial;mesothelial cell
Blood cells
HEK
Platelet
MitoPedia: SUIT

Steps and respiratory states

SUIT-010

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2Rot ROX ce1;ce2Rot
ce3S SL(n) S CII ce1;ce2Rot;ce3S
ce4D SP S CII ce1;ce2Rot;ce3S;ce4D
1Dig SP S CII ce1;ce2Rot;ce3S;ce4D;1Dig
1c SP S CII ce1;ce2Rot;ce3S;ce4D;1Dig;1c


Questions.jpg


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Strengths and limitations

Compare SUIT protocols


MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry