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SUIT-010

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high-resolution terminology - matching measurements at high-resolution


SUIT-010

Description

Respirometric test of optimum digitonin concentration

Abbreviation: ceS,S

Reference: A Bioblast pdf »Versions

SUIT-category: ceS,S
SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c

DLP applications


References

 YearReferenceOrganismTissue;cell
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3Human
Mouse
Rat
Saccharomyces cerevisiae
Heart
Skeletal muscle
Endothelial;epithelial;mesothelial cell
Blood cells
HEK
Platelet
MitoPedia: SUIT

Steps and respiratory states

SUIT-010

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2Rot ROX ce1;ce2Rot
ce3S SL(n) S CII ce1;ce2Rot;ce3S
ce4D SP S CII ce1;ce2Rot;ce3S;ce4D
1Dig SP S CII ce1;ce2Rot;ce3S;ce4D;1Dig
1c SP S CII ce1;ce2Rot;ce3S;ce4D;1Dig;1c


Questions.jpg


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Strengths and limitations

  • Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.

Compare SUIT protocols


MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry