Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

SUIT-022

From Bioblast


high-resolution terminology - matching measurements at high-resolution


SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf Β»Versions


O2k-Application: O2

SUIT-category: ce
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023).


Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-022 O2 ce D051

Ce1;ce2KCN;ce3SHAM.png D051 O2 traces.png


Strengths and limitations

  • SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome c pathway, it must be carried out in parallel to SUIT-023.
+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry