Difference between revisions of "SUIT-023"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=Living cells AOX (SHAM+CN) | ||
|description= [[File:ce1;ce2SHAM;ce3KCN.png|400px]]| | |description= [[File:ce1;ce2SHAM;ce3KCN.png|400px]]| | ||
|info='''A: Determination of the respiration due to the cytochrome ''c'' oxidase pathway''' | |info='''A: Determination of the respiration due to the cytochrome ''c'' oxidase pathway''' | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' ce1;ce2SHAM;ce3KCN | |||
::: '''[[SUIT protocol pattern]]:''' | |||
The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[Complex_IV| cytochrome ''c'' oxidase (CIV)]] pathway, which is non-resistant to [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the [[alternative oxidase]] pathway (see [[SUIT-022]]). | The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[Complex_IV| cytochrome ''c'' oxidase (CIV)]] pathway, which is non-resistant to [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the [[alternative oxidase]] pathway (see [[SUIT-022]]). | ||
__TOC__ | __TOC__ | ||
Communicated by [[Huete-Ortega M]] | Communicated by [[Huete-Ortega M]], [[Gnaiger E]] (last update 2019-06-05) | ||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
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[[File:ce1;ce2SHAM;ce3KCN.png|400px]] [[File:ce1;ce2SHAM;ce3KCN.v2.png|400px]] | [[File:ce1;ce2SHAM;ce3KCN.png|400px]] [[File:ce1;ce2SHAM;ce3KCN.v2.png|400px]] | ||
[[File:D053_O2_traces.png|400px]] | [[File:D053_O2_traces.png|400px]] | ||
* [[SUIT-023 O2 ce D053]] for | * [[SUIT-023 O2 ce D053]] for living microalgal cells (ce) | ||
{{Template:SUIT-023 O2 ce D053}} | {{Template:SUIT-023 O2 ce D053}} | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] | ::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] living cells | ||
Revision as of 12:20, 5 June 2019
Description
Abbreviation: Living cells AOX (SHAM+CN)
Reference: A: Determination of the respiration due to the cytochrome c oxidase pathway
- SUIT protocol pattern: ce1;ce2SHAM;ce3KCN
The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome c oxidase (CIV) pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M, Gnaiger E (last update 2019-06-05)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for living microalgal cells (ce)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROUTINE OR ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c oxidase (CIV) pathway in cells. To complement the study with the estimation of the mitochondrial respiration due to the alternative oxidase pathway, it must be carried out in parallel to SUIT-022.
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 for microalgal living cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry