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Difference between revisions of "SUIT reference protocol"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=SUIT-RP
|abbr=SUIT-RP
|description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' (SUIT-RP1 and SUIT-RP2) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
|description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
|info=[[MiPNet21.06 SUIT reference assay]]
|info=[[MiPNet21.06 SUIT reference assay]]
}}
}}
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== Cross-linked respiratory states ==
== Cross-linked respiratory states ==
The cross-linked respiratory states for harmonization between protocols are:
The cross-linked respiratory states for harmonization between protocols are:
* (RP1:6S, RP2:7U) [[PGMSOct]]<sub>''E''</sub>
* ([[SUIT-RP1]]:6S, [[SUIT-RP2]]:7U) [[PGMSOct]]<sub>''E''</sub>
* (RP1:8Gp, RP2:9Rot) [[SGp]]<sub>''E''</sub>
* ([[SUIT-RP1]]:8Gp, [[SUIT-RP2]:9Rot) [[SGp]]<sub>''E''</sub>
* (10Tm or 11Tm) [[Complex IV single step |CIV]]<sub>''E''</sub>
* (10Tm or 11Tm) [[Complex IV single step |CIV]]<sub>''E''</sub>



Revision as of 13:39, 24 January 2016


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.

Abbreviation: SUIT-RP

Reference: MiPNet21.06 SUIT reference assay


MitoPedia methods: "SUIT protocol" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property. SUIT protocol"SUIT protocol" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property. 



Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:


SUIT RP1


SUIT RP2