Difference between revisions of "SUIT reference protocol"
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# SUIT-RP1: [[PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd]] | # SUIT-RP1: [[PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd]] | ||
# SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]] | # SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]] | ||
|info=[[MiPNet21.06 SUIT reference assay]] | |info=[[MiPNet21.06 SUIT reference assay]] | ||
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{{MitoPedia methods | {{MitoPedia methods}} | ||
|mitopedia | {{MitoPedia SUIT | ||
|mitopedia SUIT=SUIT protocol | |||
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{{MitoPedia topics}} | {{MitoPedia topics}} |
Revision as of 15:26, 25 January 2016
Description
The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
- SUIT-RP1: PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd
- SUIT-RP2: D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd
Abbreviation: SUIT-RP
Reference: MiPNet21.06 SUIT reference assay
Cross-linked respiratory states
The cross-linked respiratory states for harmonization between protocols are: