Difference between revisions of "SUIT reference protocol"
From Bioblast
| Line 3: | Line 3: | ||
|description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis. | |description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis. | ||
: SUIT-RP1: [[ | : SUIT-RP1: [[NFSGpCIV 1PM 2D 3c 4U 5Oct 6G 7S 8Rot 9Gp 10Ama 11Tm 12Azd]] | ||
: SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]] | : SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]] | ||
|info=[[MiPNet21.06 SUIT reference assay]] | |info=[[MiPNet21.06 SUIT reference assay]] | ||
| Line 16: | Line 15: | ||
}} | }} | ||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
[[File:SUIT-ETS-input NFSGp.jpg|right|300px|link=MiPNet21.06 SUIT reference assay |SUIT reference protocols]] | |||
== Cross-linked respiratory states == | == Cross-linked respiratory states == | ||
The cross-linked respiratory states for harmonization between protocols are: | The cross-linked respiratory states for harmonization between protocols are: | ||
Revision as of 10:34, 30 January 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT reference protocol
Description
The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
Abbreviation: SUIT-RP
Reference: MiPNet21.06 SUIT reference assay
Cross-linked respiratory states
The cross-linked respiratory states for harmonization between protocols are:
