Difference between revisions of "SUIT reference protocol"
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|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT RP1]] and [[SUIT RP2]]). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis. | |description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT RP1]] and [[SUIT RP2]]). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis. | ||
: [[SUIT RP1]]: | : [[SUIT RP1]]: FNSGpCIV 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp 10Ama 11Tm 12Azd | ||
: [[SUIT RP2]]: | : [[SUIT RP2]]: FNSGpCIV 1D 2Oct 3M 4c 5P 6G 7S 8Gp 9U 10Rot 11Ama 12Tm 13Azd | ||
|info=[[MiPNet21.06 SUIT RP]] | |info=[[MiPNet21.06 SUIT RP]] | ||
}} | }} | ||
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|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol | ||
}} | }} | ||
[[File:SUIT-Catg NFSGp.jpg|right|300px|SUIT-Catg]] | [[File:SUIT-Catg NFSGp.jpg|right|300px|SUIT-Catg]] | ||
== Cross-linked respiratory states == | == Cross-linked respiratory states == |
Revision as of 22:25, 21 August 2016
Description
The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two harmonized SUIT protocols (SUIT RP1 and SUIT RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.
- SUIT RP1: FNSGpCIV 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp 10Ama 11Tm 12Azd
- SUIT RP2: FNSGpCIV 1D 2Oct 3M 4c 5P 6G 7S 8Gp 9U 10Rot 11Ama 12Tm 13Azd
Abbreviation: SUIT RP
Reference: MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol