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Difference between revisions of "SUIT reference protocol"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=SUIT RP
|abbr=SUIT RP
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]] - SUIT RP1 and [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-]] - SUIT RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT-001|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]] - SUIT RP1 and [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-]] - SUIT RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.


: SUIT_RP1: [[SUIT-001|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]] - 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
: SUIT_RP1: [[SUIT-001|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]] - 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
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== Cross-linked respiratory states ==
== Cross-linked respiratory states ==
:::: The cross-linked respiratory states for harmonization between protocols are:
:::: The cross-linked respiratory states for harmonization between protocols are:
::::* [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]: 8Gp, [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot- |SUIT RP2]]: 8Rot - [[SGp]]<sub>''E''</sub>
::::* [[SUIT-001|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]: 8Gp, [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot- |SUIT RP2]]: 8Rot - [[SGp]]<sub>''E''</sub>
::::* CIV - [[Complex IV single step |Tm]]<sub>''E''</sub>
::::* CIV - [[Complex IV single step |Tm]]<sub>''E''</sub>



Revision as of 10:54, 27 May 2019


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two harmonized SUIT protocols (1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- - SUIT RP1 and 1D;2OctM;3P;4G;5S;6Gp;7U;8Rot- - SUIT RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT_RP1: 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- - 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
SUIT_RP2: 1D;2OctM;3P;4G;5S;6Gp;7U;8Rot- - 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Abbreviation: SUIT RP

Reference: MiPNet21.06 SUIT RP


MitoPedia concepts: SUIT concept 

SUIT-catg

Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:

SUIT RP