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Difference between revisions of "SUIT reference protocol"

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{{MitoPedia
{{MitoPedia
|abbr=SUIT-RP
|abbr=SUIT RP
|description=The [[SUIT reference assay]] consists of two '''SUIT reference protocols''' ([[SUIT-RP1]] and [[SUIT-RP2]]) which are [[harmonized SUIT protocols]]. These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT-001]] - RP1 and [[SUIT-002]] - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.


: SUIT-RP1: [[PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd]]
: [[SUIT-001]] (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
: SUIT-RP1: [[1PM(L2Pc3E)_4Oct_5G_6S_7Rot_8Gp_9Ama_10Tm_11Azd]]


: SUIT-RP2: [[D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd]]
: [[SUIT-002]] (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
: SUIT-RP2: [[1D(ROX)_2Oct(P)_3M_4Pc_5G_6S(P7E)_8Gp_9Rot_10Ama_11Tm_12Azd]]
|info=[[Doerrier 2018 Methods Mol Biol]], [[MiPNet21.06 SUIT RP]]
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|info=[[MiPNet21.06 SUIT reference assay]]
}}
}}
{{MitoPedia methods}}
{{MitoPedia concepts
{{MitoPedia SUIT
|mitopedia concept=SUIT concept
|mitopedia SUIT=SUIT protocol
}}
}}
{{MitoPedia topics}}
[[File:SUIT-catg FNSGpCIV.jpg|right|300px|SUIT-catg]]
== Cross-linked respiratory states ==
== Cross-linked respiratory states ==
The cross-linked respiratory states for harmonization between protocols are:
:::: The cross-linked respiratory states for harmonization between protocols are:
* [[SUIT-RP1]]:6S and [[SUIT-RP2]]:7U - [[PGMSOct]]<sub>''E''</sub>
::::* [[SUIT-001]]: 8Gp, [[SUIT-002]]: 8Rot - [[SGp]]<sub>''E''</sub>
* [[SUIT-RP1]]:8Gp, [[SUIT-RP2]:9Rot - [[SGp]]<sub>''E''</sub>
::::* CIV - [[Complex IV single step |Tm]]<sub>''E''</sub>
* 10Tm or 11Tm - [[Complex IV single step |CIV]]<sub>''E''</sub>
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[[File:SUIT-Reference-Protocol 1.jpg|700px |SUIT RP1]]<br />


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[[File:SUIT-Reference-Protocol 2.jpg|700px |SUIT RP2]]<br />
[[File:SUIT-Reference-Protocol.jpg|500px |SUIT RP]]
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Latest revision as of 09:38, 29 July 2022


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two harmonized SUIT protocols (SUIT-001 - RP1 and SUIT-002 - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT-001 (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
SUIT-002 (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Abbreviation: SUIT RP

Reference: Doerrier 2018 Methods Mol Biol, MiPNet21.06 SUIT RP


MitoPedia concepts: SUIT concept 

SUIT-catg

Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:

SUIT RP