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Difference between revisions of "SUIT reference protocol"

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{{MitoPedia
{{MitoPedia
|abbr=SUIT RP
|abbr=SUIT RP
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT RP1]] and [[SUIT RP2]]). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT-001]] - RP1 and [[SUIT-002]] - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.


: [[SUIT RP1]]: FNSGpCIV 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp 10Ama 11Tm 12Azd
: [[SUIT-001]] (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd


: [[SUIT RP2]]: FNSGpCIV 1D 2Oct 3M 4c 5P 6G 7S 8Gp 9U 10Rot 11Ama 12Tm 13Azd
: [[SUIT-002]] (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
|info=[[MiPNet21.06 SUIT RP]]
|info=[[Doerrier 2018 Methods Mol Biol]], [[MiPNet21.06 SUIT RP]]
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=SUIT protocol
|mitopedia concept=SUIT concept
}}
}}
[[File:SUIT-Catg NFSGp.jpg|right|300px|SUIT-Catg]]
[[File:SUIT-catg FNSGpCIV.jpg|right|300px|SUIT-catg]]
== Cross-linked respiratory states ==
== Cross-linked respiratory states ==
:::: The cross-linked respiratory states for harmonization between protocols are:
:::: The cross-linked respiratory states for harmonization between protocols are:
::::* [[SUIT RP1]]: 9Gp, [[SUIT RP2]]: 10Rot - [[SGp]]<sub>''E''</sub>
::::* [[SUIT-001]]: 8Gp, [[SUIT-002]]: 8Rot - [[SGp]]<sub>''E''</sub>
::::* 11Tm and 12Tm - [[Complex IV single step |Tm]]<sub>''E''</sub>
::::* CIV - [[Complex IV single step |Tm]]<sub>''E''</sub>


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[[File:SUIT-Reference-Protocol.jpg|700px |SUIT RP]]
[[File:SUIT-Reference-Protocol.jpg|500px |SUIT RP]]
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Latest revision as of 09:38, 29 July 2022


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two harmonized SUIT protocols (SUIT-001 - RP1 and SUIT-002 - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT-001 (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
SUIT-002 (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Abbreviation: SUIT RP

Reference: Doerrier 2018 Methods Mol Biol, MiPNet21.06 SUIT RP


MitoPedia concepts: SUIT concept 

SUIT-catg

Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:

SUIT RP