SUIT reference protocol

high-resolution terminology - matching measurements at high-resolution

SUIT reference protocol

Description

The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT-RP1: NFSGpCIV 1PM 2D 3c 4U 5Oct 6G 7S 8Rot 9Gp 10Ama 11Tm 12Azd

SUIT-RP2: D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd

Abbreviation: SUIT-RP

Reference: MiPNet21.06 SUIT reference assay

MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 

Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:

• SUIT-RP1:6S and SUIT-RP2:7U - PGMSOct_E
• SUIT-RP1:8Gp, [[SUIT-RP2]:9Rot - SGp_E
• 10Tm or 11Tm - CIV_E

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