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SUIT reference protocol

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Revision as of 13:24, 11 February 2016 by Gnaiger Erich (talk | contribs)


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT-RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT-RP consists of two harmonized SUIT protocols (SUIT-RP1 and SUIT-RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT-RP1: NFSGpTm 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp 10Ama 11Tm 12Azd
SUIT-RP2: NFSGpTm 1D 2Oct 3M 4c 5P 6G 7S 8Gp 9U 10Rot 11Ama 12Tm 13Azd

Abbreviation: SUIT-RP

Reference: MiPNet21.06 SUIT reference protocol





MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 



SUITp-Catg

Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:


SUIT RP