Difference between revisions of "SUITbrowser"

Revision as of 12:00, 14 July 2021

high-resolution terminology - matching measurements at high-resolution

SUITbrowser

Description

Use the SUITbrowser to find the best substrate-uncoupler-inhibitor-titration (SUIT) protocol for your research questions.

Open the SUITbrowser: http://suitbrowser.oroboros.at/

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.

Define your experimental strategy

Mitochondrial preparations or living cells

Mitochondrial preparations: imt, thom, pce, pti (including pfi)
Living cells: ce = vce + dce

Applications

O₂
H₂O₂ and O₂
Mitochondrial ATP production and O₂
Membrane potential and O₂

Research questions

The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:

Coupling control

OXPHOS respiration

ROUTINE respiration

Maximum physiological capacity

Cell viability test

Crabtree effect

AOX activity

Permeable succinate/malonate

Carbohydrate

Fatty acid oxidation

Glutamine/glutamate

Succinate pathway

Glycerophosphate

Q-junction (NS)

Q-junction (FNS)

Q-junction (FNSGp)

CI threshold

Complex IV activity

mt outer membrane integrity

Unspecific ATPase activity

Optimum digitonin concentration

Mitochondrial ATP production

Mitochondrial membrane potential

H₂O₂ - membrane potential dependence

H₂O₂ - [O₂] dependence

H₂O₂ - RET initiated

H₂O₂ - NADH-pathway initiated

MitoPedia concepts: SUIT concept, SUIT protocol

MitoPedia methods: Respirometry, Fluorometry

MitoPedia O2k and high-resolution respirometry: DatLab

@@ Line 3: / Line 3: @@
   Open the SUITbrowser: http://suitbrowser.oroboros.at/
+}}
+<br />
+<br />
+{{Technical support integrated}}
+__TOC__
+:::: Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.
-Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending of the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple reasearch questions at a time, to find the best protocols that adress this combination of problems.
-}}
-{{MitoPedia concepts
-|mitopedia concept=SUIT concept, SUIT protocol
-}}
-{{MitoPedia methods
-|mitopedia method=Respirometry, Fluorometry
-}}
-{{MitoPedia O2k and high-resolution respirometry
-|mitopedia O2k and high-resolution respirometry=DatLab
-}}
-{{MitoPedia topics}}
 == Define your experimental strategy ==
@@ Line 34: / Line 30: @@
-::::* [[Coupling control state|Coupling control]]
+::::* [[Coupling-control state|Coupling control]]
+::::* [[Oxidative phosphorylation|OXPHOS respiration]]
+::::* [[ROUTINE respiration]]
+::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]]
-::::* [[Oxidative phosphorylation|Physiological pathway control]]
+::::* [[Living cells|Cell viability test]]
+::::* [[Crabtree effect]]
+::::* [[Alternative oxidase|AOX activity]]
+::::* [[MitoKit-CII|Permeable succinate/malonate]]
 ::::* [[Carbohydrate]]
@@ Line 42: / Line 50: @@
 ::::* [[Fatty acid oxidation]]
-::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]]
+::::* [[Glutamate-anaplerotic pathway control state|Glutamine/glutamate]]
-::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]]
-::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Physiological capacity]]
 ::::* [[Succinate|Succinate pathway]]
@@ Line 58: / Line 62: @@
 ::::* [[Q-junction|Q-junction (FNSGp)]]
-::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV (CIV)]]
+::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]]
+::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV activity]]
 ::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]]
-::::* [[Living cells|Cell viability test]]
+::::* [[ATPases|Unspecific ATPase activity]]
+::::* [[Digitonin|Optimum digitonin concentration]]
+::::* [[Magnesium Green|Mitochondrial ATP production]]
+::::* [[Mitochondrial membrane potential]]
+::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - membrane potential dependence]]
+::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - [O<sub>2</sub>] dependence]]
+::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - RET initiated]]
+::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - NADH-pathway initiated]]
-::::* [[Crabtree effect]]
+{{MitoPedia concepts
+|mitopedia concept=SUIT concept, SUIT protocol
+}}
+{{MitoPedia methods
+|mitopedia method=Respirometry, Fluorometry
+}}
+{{MitoPedia O2k and high-resolution respirometry
+|mitopedia O2k and high-resolution respirometry=DatLab
+}}
 [[Category:SUITbrowser]]