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Difference between revisions of "Sample"

From Bioblast
Line 14: Line 14:
'''Addition of sample to the respirometer chamber:'''
'''Addition of sample to the respirometer chamber:'''
:::'''[[Isolated_mitochondria|Isolated mitochondria]] (imt):''' Imt are typically added with a Hamilton syringe. Care should be taken to not exert damaging lowย  pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
:::'''[[Isolated_mitochondria|Isolated mitochondria]] (imt):''' Imt are typically added with a Hamilton syringe. Care should be taken to not exert damaging lowย  pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
:::'''[[Intact_cells|Intact_cells]] (vce):''' Vce are typically added with a Hamilton syringe. Care should be taken to not exert damaging lowย  pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
:::'''[[Intact_cells|Intact cells]] (vce):''' Vce are typically added with a Hamilton syringe. Care should be taken to not exert damaging lowย  pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
:::'''Very large and/or fragile [[Intact_cells|Intact_cells]] (vce):''' Some cell types may be easily damaged when injected with a syringe. For these we recommend: remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.
:::'''Very large and/or fragile [[Intact_cells|Intact cells]] (vce):''' Some cell types may be easily damaged when injected with a syringe. For these we recommend: remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.
:::'''[[PBMC]]s and [[Platelet]]s''': We recommend adding 2*10^6/mL PBMCs, 200*10^6/mL PLTs.
:::'''[[PBMC]]s and [[Platelet]]s''': We recommend adding 2*10^6/mL PBMCs, 200*10^6/mL PLTs.
:::'''[[Permeabilized_tissue_or_cells]] (pti)''': For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again. If permeabilized cells can be loaded into a syringe, they may be injected with a Hamilton syringe. If the cells are rather fragile, remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.
:::'''[[Permeabilized tissue]] (pti)''': For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.
:::'''[[Permeabilized_muscle_fibres]] (pfi)''': Remove the Stopper, add the permeabilized fibers into the O2k-Chamber, close the chamber again.
:::'''[[Permeabilized muscle fibres]] (pfi)''': Remove the Stopper, add the permeabilized fibers into the O2k-Chamber, close the chamber again.
:::'''[[Permeabilized cells]] (pce)''': If the cells can be loaded into a syringe, they may be injected with a Hamilton syringe. If the cells are rather fragile, remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.





Revision as of 15:03, 20 March 2019


high-resolution terminology - matching measurements at high-resolution


Sample

Description

A sample is one or more parts taken from a system and intended to provide information on the system, often to serve as a basis for evaluation of the system (diagnosis) or for a decision on intervention (therapy, production process). Compare: Primary sample.


Reference: ISO 15189:2012 Medical laboratories โ€” Particular requirements for quality and competence


MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 


MitoPedia topics: Sample preparation 


Application in HRR

Addition of sample to the respirometer chamber:

Isolated mitochondria (imt): Imt are typically added with a Hamilton syringe. Care should be taken to not exert damaging low pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
Intact cells (vce): Vce are typically added with a Hamilton syringe. Care should be taken to not exert damaging low pressure during aspiration of sample into the syringe or exerting high pressure during injection into the chamber.
Very large and/or fragile Intact cells (vce): Some cell types may be easily damaged when injected with a syringe. For these we recommend: remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.
PBMCs and Platelets: We recommend adding 2*10^6/mL PBMCs, 200*10^6/mL PLTs.
Permeabilized tissue (pti): For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.
Permeabilized muscle fibres (pfi): Remove the Stopper, add the permeabilized fibers into the O2k-Chamber, close the chamber again.
Permeabilized cells (pce): If the cells can be loaded into a syringe, they may be injected with a Hamilton syringe. If the cells are rather fragile, remove the Stopper, replace a defined volume of the respiration medium with cell suspension, close the chamber again.


MitoFit Quality Control System

Communicated by Gnaiger E 2016-01-27; last update 2016-05-28.
Sample and sample type
A sample of a defined sample type subjected to a specific sample preparation (see MitoPedia: Sample preparations) may be, e.g., a mitochondrial preparation obtained from an organ, cells suspended from a cell culture dish, a tissue biopsy, an organ, an individual organism, or a group of organisms (e.g. a number of nematodes studied collectively in an experimental chamber).
Example: Sample and subsample
A blood sample or a biopsy taken from an individual organism. The sample size, N, corresponds to the number replica, e.g. N organisms in a study group (arm). A sample may be processed and split into a number of subsamples (e.g. smaller volumes of serum taken from a larger volume of serum) for (i) application of different types of assay, and (ii) a number of repetitions, n, of the same assay on the same sample.