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Difference between revisions of "Setting the oxygen concentration"

From Bioblast
(Created page with "{{Technical support}} :::: Question: Can we set the oxygen concentration in the Oroboros-2k to desired levels? :::: Answer: Yes. - It is always possible to increase the oxyge...")
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{{Technical support}}
{{Technical support}}
:::: Question: Can we set the oxygen concentration in the Oroboros-2k to desired levels?
:::: '''Question:''' Can we set the oxygen concentrations in the O2k to desired levels?


:::: Answer: Yes. - It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described inย  [[MiPNet14.13 Medium-MiR06| MiPNet14.3]]. By using the [[TIP2k]] the oxygen concentration can be maintained between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP syringes. We call this an "oxystat" approach and supply appropriate templates for controlling the TIP2k. See also [[MiPNet12.10]]. ย 
:::: '''Answer: ''' Yes. - It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H<sub>2</sub>O<sub>2</sub>, as described inย  [[MiPNet14.13 Medium-MiR06| MiPNet14.3]]. By using the [[TIP2k]], the oxygen concentration can be maintained between well defined limits, either using H<sub>2</sub>O<sub>2</sub> or (for very low oxygen concentrations) air saturated medium in the [https://www.bioblast.at/index.php/Microsyringe%5C500_mm3%5CTIP2k TIP2k microsyringes]. Such specification is called "oxystat" approach and supply appropriate templates for controlling the TIP2k. See also [[MiPNet12.10]]. ย 


:::: This leaves the question of how low oxygen levels can be reached in the first place: In a biological experiment this may be done by inserting a N2 bubble into the chamber, waiting until the desired level of oxygen is reached and then destroying the air bubble by closing the stoppers. However, most often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen and then starting the "oxystat".
::::Low oxygen levels can be reached depending on the application: (1) On a biological experiment this may be performed by injection of N2 in the gas phase, followed by closing the stoppers once the desired level of oxygen is reached. However, often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen, when the "oxystat" follows.
:::: For calibration and instrumental oxygen background purposes we use Dithionite to reduce oxygen levels, however this is not recommended in the presence of biological samples.
:::: For calibration and for [[MiPNet14.06 Instrumental O2 background |instrumental O<sub>2</sub> background tests]] purposes Dithionite is used to reduce oxygen levels, however this is not recommended in the presence of biological samples.

Revision as of 09:39, 8 June 2020


                  


O2k-Open Support

Setting the oxygen concentration



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Question: Can we set the oxygen concentrations in the O2k to desired levels?
Answer: Yes. - It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described in MiPNet14.3. By using the TIP2k, the oxygen concentration can be maintained between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP2k microsyringes. Such specification is called "oxystat" approach and supply appropriate templates for controlling the TIP2k. See also MiPNet12.10.
Low oxygen levels can be reached depending on the application: (1) On a biological experiment this may be performed by injection of N2 in the gas phase, followed by closing the stoppers once the desired level of oxygen is reached. However, often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen, when the "oxystat" follows.
For calibration and for instrumental O2 background tests purposes Dithionite is used to reduce oxygen levels, however this is not recommended in the presence of biological samples.